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Literature summary for 3.4.22.1 extracted from

  • Siricoon, S.; Vichasri Grams, S.; Lertwongvisarn, K.; Abdullohfakeeyah, M.; Smooker, P.M.; Grams, R.
    Fasciola gigantica cathepsin B5 is an acidic endo- and exopeptidase of the immature and mature parasite (2015), Biochimie, 119, 6-15 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
ProFgCB5 is expressed as a recombinant, N-terminal His-tagged protein in Escherichia coli M15. Expression in Pichia pastoris Fasciola gigantica

Inhibitors

Inhibitors Comment Organism Structure
cystatin C
-
Fasciola gigantica
stefin-1
-
Fasciola gigantica
stefin-2
-
Fasciola gigantica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2622
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin 37°C, pH 4.5 Fasciola gigantica

Organism

Organism UniProt Comment Textmining
Fasciola gigantica A0A0U4HGR1
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cecal epithelium expressed in later juvenile stages and the mature worm Fasciola gigantica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
Fasciola gigantica benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
?
additional information the enzyme shows substantial exopeptidase activity at a relative rate of 34.26% compared to human cathepsin B Fasciola gigantica ?
-
?

Synonyms

Synonyms Comment Organism
cathepsin B5
-
Fasciola gigantica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Fasciola gigantica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.5
-
maximal stability Fasciola gigantica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Fasciola gigantica

pH Range

pH Minimum pH Maximum Comment Organism
3 6 pH 3.0: about75 % of maximal activity, pH 6.0: about 50% of maximal activity Fasciola gigantica

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00013
-
37°C, pH 4.5 Fasciola gigantica stefin-2
0.000486
-
37°C, pH 4.5 Fasciola gigantica cystatin C
0.00068
-
37°C, pH 4.5 Fasciola gigantica stefin-1

General Information

General Information Comment Organism
physiological function the enzyme could be stable and highly active in the endolysosomal pathway degrading endocytosed peptides predigested by cathepsin L in the gut lumen whereas as a secreted protease it might be short lived but still stimulating an immune response. Due to its exopeptidase activity it could be involved in the regulative processing of other proteins. It is not involved in the immediate infection process Fasciola gigantica