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Literature summary for 3.4.22.1 extracted from

  • Deval, C.; Bechet, D.; Obled, A.; Ferrara, M.
    Purification and properties of different isoforms of bovine cathepsin B (1990), Biochem. Cell Biol., 68, 822-826.
    View publication on PubMed
Search for more literature for 3.4.22.1

Activating Compound

Activating Compound Comment Organism Structure
cysteine activates Bos taurus
dithiothreitol activates Bos taurus
thiol compounds
-
 Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
L-3-carboxy-2,3-trans-epoxypropionyl-leucylamido-(4-guanidino)butane E-64 Bos taurus
leupeptin
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.114 0.125 benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 1 * 25000 + 1 * 5000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Proteins + H2O Bos taurus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Bos taurus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Bos taurus carbobenzoxy-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
Proteins + H2O
-
 Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 25000 + 1 * 5000, SDS-PAGE Bos taurus
dimer heavy and light chain result from cleavage of mature cathepsin B Bos taurus
monomer 1*30000, SDS-PAGE Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
52 75 benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide
-
 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7
-
 Bos taurus