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Literature summary for 3.4.22.1 extracted from

  • Kamboj, R.C.; Pal, S.; Singh, H.
    Purification and characterization of cathepsin B from goat brain (1990), J. Biosci., 15, 397-408.
No PubMed abstract available
Search for more literature for 3.4.22.1

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activates Capra hircus

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl disulfide
-
 Capra hircus
antipain
-
 Capra hircus
Dimethylsulfoxide
-
 Capra hircus
Hg2+
-
 Capra hircus
iodoacetamide
-
 Capra hircus
iodoacetic acid
-
 Capra hircus
L-3-carboxy-2,3-trans-epoxypropionyl-leucylamido-(4-guanidino)butane E-64 Capra hircus
leupeptin
-
 Capra hircus
Mg2+
-
 Capra hircus
N-tosyl-lysyl chloromethylketone
-
 Capra hircus
N-tosyl-phenylalanine chloromethylketone
-
 Capra hircus
p-chloromercuribenzoate
-
 Capra hircus
Zn2+
-
 Capra hircus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09 0.29 benzyloxycarbonyl-Arg-Arg-4-methoxy-beta-naphthylamide depending on concentration of dimethylsulfoxide, 1-2.5% Capra hircus
0.51 2.36 benzoyl-DL-Arg-beta-naphthylamide depending on concentration of dimethylsulfoxide, 1-2.5% Capra hircus

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Capra hircus 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000 29000 gel filtration, SDS-PAGE Capra hircus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Proteins + H2O Capra hircus turnover of intracellular proteins ?
-
 ?
Proteins + H2O Capra hircus enzyme activation and conversion of precursor proteins to their active form ?
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
dimethylsulfoxide 50% of activity lost at 6% Capra hircus
dimethylsulfoxide inhibits Capra hircus

Organism

Organism UniProt Comment Textmining
Capra hircus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification glycoprotein Capra hircus

Purification (Commentary)

Purification (Comment) Organism
-
 Capra hircus

Source Tissue

Source Tissue Comment Organism Textmining
cerebrum maximum activity in cerebrum Capra hircus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10
-
 benzoyl-DL-Arg-beta-naphthylamide as substrate, in presence of 2.5% dimethylsulfoxide Capra hircus
40
-
 benzyloxycarbonyl-Arg-Arg-4-methoxy-beta-naphthylamide as substrate, in presence of 2.5% dimethylsulfoxide Capra hircus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-beta-naphthylamide + H2O
-
 Capra hircus Arg + beta-naphthylamine
-
 ?
benzoyl-DL-Arg-beta-naphthylamide + H2O
-
 Capra hircus benzoyl-DL-Arg + beta-naphthylamine
-
 ?
benzoyl-L-Arg-beta-naphthylamide + H2O
-
 Capra hircus benzoyl-L-Arg + beta-naphthylamine
-
 ?
benzoyl-L-Arg-p-nitroanilide + H2O
-
 Capra hircus benzoyl-L-Arg + p-nitroaniline
-
 ?
benzyloxycarbonyl-Ala-Arg-Arg-4-methoxy-beta-naphthylamide + H2O
-
 Capra hircus benzyloxycarbonyl-Ala-Arg-Arg + 4-methoxy-beta-naphthylamine
-
 ?
benzyloxycarbonyl-Arg-Arg-4-methoxy-beta-naphthylamide + H2O
-
 Capra hircus benzyloxycarbonyl-Arg-Arg + 4-methoxy-beta-naphthylamine
-
 ?
benzyloxycarbonyl-Arg-Arg-p-nitroanilide + H2O
-
 Capra hircus benzyloxycarbonyl-Arg-Arg + p-nitroaniline
-
 ?
benzyloxycarbonyl-Phe-Arg-4-methoxy-beta-naphthylamide + H2O
-
 Capra hircus benzyloxycarbonyl-Phe-Arg + 4-methoxy-beta-naphthylamine
-
 ?
benzyloxycarbonyl-Phe-Arg-p-nitroanilide + H2O
-
 Capra hircus benzyloxycarbonyl-Phe-Arg + p-nitroaniline
-
 ?
benzyloxycarbonyl-Val-Lys-Lys-Arg-4-methoxy-beta-naphthylamide + H2O
-
 Capra hircus ? + 4-methoxy-beta-naphthylamine
-
 ?
Proteins + H2O turnover of intracellular proteins Capra hircus ?
-
 ?
Proteins + H2O enzyme activation and conversion of precursor proteins to their active form Capra hircus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Capra hircus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 fairly stable up to Capra hircus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6 less stable below and above Capra hircus