Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | activated prostasin appears to target several downstream effector proteins, including the epithelial sodium channel and the G protein-coupled protease activated receptor-2, which are both matriptase substrates | ? | - |
? | |
proform epithelial sodium channel + H2O | Homo sapiens | - |
mature epithelial sodium channel + ? | - |
? | |
proform G protein-coupled protease activated receptor-2 + H2O | Homo sapiens | - |
mature G protein-coupled protease activated receptor-2 + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16651 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | matriptase is an effective activator of the prostasin zymogen through direct proteolytic cleavage at its zymogen activation site. Prostasin is required for matriptase activation in intestinal epithelial cells to regulate closure of the paracellular pathway | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Caco-2 cell | - |
Homo sapiens | - |
epidermis | - |
Homo sapiens | - |
epithelium | - |
Homo sapiens | - |
intestine | - |
Homo sapiens | - |
additional information | prostasin is co-expressed with matriptase and increases during human Caco-2 cell differentiation and barrier formation | Homo sapiens | - |
skin | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | activated prostasin appears to target several downstream effector proteins, including the epithelial sodium channel and the G protein-coupled protease activated receptor-2, which are both matriptase substrates | Homo sapiens | ? | - |
? | |
additional information | the matriptase zymogen is no substrate of prostasin | Homo sapiens | ? | - |
? | |
proform epithelial sodium channel + H2O | - |
Homo sapiens | mature epithelial sodium channel + ? | - |
? | |
proform G protein-coupled protease activated receptor-2 + H2O | - |
Homo sapiens | mature G protein-coupled protease activated receptor-2 + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | prostasin expression increases during human Caco-2 cell differentiation and barrier formation | up |
General Information | Comment | Organism |
---|---|---|
metabolism | matriptase and not prostasin is the primary effector protease of tight junction assembly in simple columnar epithelia | Homo sapiens |
physiological function | Prostasin catalyzed activation of ENaC induces an open channel conformation associated with sodium influx that results in membrane depolarization. Prostasin together with matriptase comprise a single common proteolytic pathway,that is required for barrier formation. Prostasin is activated through proteolytic cleavage by matriptase, but prostasin is also required for matriptase activation in intestinal epithelial cells to regulate closure of the paracellular pathway | Homo sapiens |