Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.B58 extracted from

  • Chavez Croocker, P.; Sako, Y.; Uchida, A.
    Purification and characterization of an intracellular heat-stable proteinase (pernilase) from the marine hyperthermophilic archaeon Aeropyrum pernix K1. (1999), Extremophiles, 3, 3-9.
    View publication on PubMed
Search for more literature for 3.4.21.B58

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, 34% inhibition Aeropyrum pernix
diisopropyl fluorophosphate 1 mM, 88% inhibition Aeropyrum pernix
EDTA 1 mM, 30% inhibition Aeropyrum pernix
EGTA 1 mM, 40% inhibition Aeropyrum pernix
PMSF 1 mM, 87% inhibition Aeropyrum pernix
Soybean trypsin inhibitor 1 mg/ml 40% inhibition Aeropyrum pernix

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Aeropyrum pernix 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel filtration Aeropyrum pernix

Organic Solvent Stability

Organic Solvent Comment Organism
2-mercaptoethanol 1%, resistant to Aeropyrum pernix
dithiothreitol 10%, resistant to Aeropyrum pernix
guanidine-HCl 4 M, complete inactivation Aeropyrum pernix
SDS 2%, 97% inactivation Aeropyrum pernix
urea 8 M, 88% inactivation Aeropyrum pernix

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix
-
-
-
Aeropyrum pernix DSM 11879
-
-
-

Oxidation Stability

Oxidation Stability Organism
highly resistant to the reducing agents dithiothreitol and 2-mercaptoethanol Aeropyrum pernix

Purification (Commentary)

Purification (Comment) Organism
-
 Aeropyrum pernix

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Aeropyrum pernix ?
-
 ?
azocasein + H2O
-
 Aeropyrum pernix DSM 11879 ?
-
 ?
benzoyl-Arg-amido-4-methyl-coumarin + H2O about 45% of the activity with Boc-Leu-Gly-Arg-amido-4-methyl-coumarin Aeropyrum pernix benzoyl-Arg + 7-amino-4-methylcoumarin
-
 ?
benzoyl-Arg-amido-4-methyl-coumarin + H2O about 45% of the activity with Boc-Leu-Gly-Arg-amido-4-methyl-coumarin Aeropyrum pernix DSM 11879 benzoyl-Arg + 7-amino-4-methylcoumarin
-
 ?
Boc-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O about 45% of the activity with Boc-Leu-Gly-Arg-amido-4-methyl-coumarin Aeropyrum pernix Boc-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
Boc-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O about 45% of the activity with Boc-Leu-Gly-Arg-amido-4-methyl-coumarin Aeropyrum pernix DSM 11879 Boc-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
Boc-Leu-Gly-Arg-amido-4-methyl-coumarin + H2O
-
 Aeropyrum pernix Boc-Leu-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
Boc-Leu-Gly-Arg-amido-4-methyl-coumarin + H2O
-
 Aeropyrum pernix DSM 11879 Boc-Leu-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
Boc-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O about 20% of the activity with Boc-Leu-Gly-Arg-amido-4-methyl-coumarin Aeropyrum pernix Boc-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
 ?
Boc-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O about 20% of the activity with Boc-Leu-Gly-Arg-amido-4-methyl-coumarin Aeropyrum pernix DSM 11879 Boc-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Aeropyrum pernix

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Aeropyrum pernix

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80 110 80°C: about 65% of maximal activity, 110°C: about 75% of maximal activity Aeropyrum pernix

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 half-life: 85 min Aeropyrum pernix
110
-
 half-life: 12 min Aeropyrum pernix

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Aeropyrum pernix