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Literature summary for 3.4.21.B57 extracted from

  • Snajder, M.; Vilfan, T.; Cernilec, M.; Rupreht, R.; Popovic, M.; Juntes, P.; Serbec, V.C., Ulrih, N.P.
    Enzymatic degradation of PrPSc by a protease secreted from Aeropyrum pernix K1 (2012), PLoS One, 7, e39548.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aeropyrum pernix

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 5 mM, 61% loss of activity in absence of CaCl2 Aeropyrum pernix
dithiothreitol 5 mM, 64% loss of activity in absence of CaCl2 Aeropyrum pernix
EDTA 5 mM, complete loss of activity Aeropyrum pernix
EGTA 5 mM, complete loss of activity Aeropyrum pernix
Guanidine HCl 5 mM, 65% loss of activity in absence of CaCl2, activation in presence of 1 mM CaCl2 Aeropyrum pernix
PMSF 5 mM, complete loss of activity Aeropyrum pernix
SDS 3%, 80% loss of activity in absence of CaCl2 Aeropyrum pernix
Urea 5 mM, 50% loss of activity in absence of CaCl2 Aeropyrum pernix

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aeropyrum pernix
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 maximum enhancement of the purified pernisine activity is observed at around 1 mM CaCl2. Further increasing the CaCl2 above 1 mM leads to a gradual decline in this enhanced activity. In the presence of 1 mM CaCl2, the proteolytic activity of pernisine is enhanced, to reach around 75% greater activity, with the maximum activity at 105.0°C Aeropyrum pernix
additional information NaCl and CaCl2 together do not show any cumulative effects, as they appear to separately affect the activity Aeropyrum pernix
NaCl the maximal activity of the purified pernisine seen for 20 mM NaCl in the absence of 1 mM CaCl2 increases to more than 2fold after the addition of CaCl2, with even larger relative enhancement by CaCl2 at higher NaCl concentrations Aeropyrum pernix

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
x * 23000, SDS-PAGE, the purified pernisine is in active mature form at 23 kDa and in pre-form at 34 kDa, it appears that the purified pernisin is a mixture of two different active forms Aeropyrum pernix
34000
-
x * 34000, SDS-PAGE, the purified pernisine is in active mature form at 23 kDa and in pre-form at 34 kDa, it appears that the purified pernisin is a mixture of two different active forms Aeropyrum pernix

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix
-
-
-
Aeropyrum pernix DSM 11879
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the purified pernisine has a proregion that is autocleaved during maturation Aeropyrum pernix

Purification (Commentary)

Purification (Comment) Organism
-
Aeropyrum pernix

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
abnormal prion protein PrP(Sc) + H2O the prion protein PrP(Sc) is a pathological prion protein PrP isoform. The proteolytic activity of pernisine does not depend on the species of origin of the PrP used (bovine, mouse, human) Aeropyrum pernix ?
-
?
abnormal prion protein PrP(Sc) + H2O the prion protein PrP(Sc) is a pathological prion protein PrP isoform. The proteolytic activity of pernisine does not depend on the species of origin of the PrP used (bovine, mouse, human) Aeropyrum pernix DSM 11879 ?
-
?
azocasein + H2O
-
Aeropyrum pernix ?
-
?
azocasein + H2O
-
Aeropyrum pernix DSM 11879 ?
-
?

Subunits

Subunits Comment Organism
? x * 23000, SDS-PAGE, the purified pernisine is in active mature form at 23 kDa and in pre-form at 34 kDa, it appears that the purified pernisin is a mixture of two different active forms Aeropyrum pernix
? x * 34000, SDS-PAGE, the purified pernisine is in active mature form at 23 kDa and in pre-form at 34 kDa, it appears that the purified pernisin is a mixture of two different active forms Aeropyrum pernix

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
substrate: azocasein, in absence of CaCl2 Aeropyrum pernix
105
-
substrate: azocasein, in the presence of 1 mM CaCl2 Aeropyrum pernix

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
58 99 active between 58 °C and 99 °C Aeropyrum pernix

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
20 min, stable in presence of CaCls, about 60% loss of activity in absence of CaCl2 Aeropyrum pernix
90
-
20 min, stable in presence of CaCl2, about 80% loss of activity in absence of CaCl2 Aeropyrum pernix

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
in the presence of 1 mM CaCl2, at 92°C Aeropyrum pernix
7
-
in absence of CaCl2, at 92°C Aeropyrum pernix

pH Range

pH Minimum pH Maximum Comment Organism
3 10 active between pH 3.0 and pH 10.0, in the presence of 1 mM CaCl2, at 92°C Aeropyrum pernix
3.5 8 active between pH 3.5 and pH 8.0, in absence of CaCl2, at 92°C Aeropyrum pernix