Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the activities in the presence of 1% SANISOL C and AMPHITOL 20 N are higher than those at the concentration of 0.1%. This may relate to the critical micelle concentration of the surfactants. Improvement of the enzyme activity in the presence of nonionic surfactants might be due to the stimulation of conformational changes in Tk-SP or the substrate, leading to activity gain | Thermococcus kodakarensis |
Application | Comment | Organism |
---|---|---|
medicine | potential application for the inactivation of abnormal prion protein PrPSc, a protease-resistant isoform of normal prion protein. PrPSc is largely unaffected by standard methods of sterilization, thus, contaminated neurosurgical instruments are the major cause of human transmissible spongiform encephalopathies | Thermococcus kodakarensis |
medicine | the enzyme may have potential application as a detergent additive to decrease the infectivity of abnormal prion protein PrPSc | Thermococcus kodakarensis |
medicine | Tk-SP-containing detergents can be developed to decrease the secondary infection risks of transmissible spongiform encephalopathies | Thermococcus kodakarensis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermococcus kodakarensis |
General Stability | Organism |
---|---|
in the presence of 0.05% (w/v) nonionic surfactants, such as EMULGEN LS-114 or RHEODOL Tw-0120 V, and 0.01% (w/v) EDTA, Tk-SP retains almost its entire initial activity | Thermococcus kodakarensis |
in the presence of anionic surfactants, the enzyme is unstable, losing up to 80% of its activity | Thermococcus kodakarensis |
in the presence of anionic surfactants, Tk-SP is unstable, losing up to 80% of its activity | Thermococcus kodakarensis |
less stable in the presence of QUARTAMIN 60 W | Thermococcus kodakarensis |
stable in the presence of 0.1% (w/v) AMPHITOL 20Y-B | Thermococcus kodakarensis |
the enzyme is highly stable in the presence of 0.05% (w/v) nonionic surfactants and 0.01% (w/v) EDTA, retaining up to 80% of its activity | Thermococcus kodakarensis |
the enzyme is highly stable in the presence of both 0.1 and 1% (w/v) nonionic surfactants | Thermococcus kodakarensis |
the enzyme retains its activity in the presence of surfactants tested at 80°C and 90°C | Thermococcus kodakarensis |
the enzyme retains more than 100% of its activity in the presence of four of the nonionic surfactants, namely, EMULGEN 147, EMULGEN LS-114, EMULGEN PP-290, and RHEODOL Tw-0120 V. It is less stable in the presence of QUARTAMIN 60 W | Thermococcus kodakarensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMPHITOL 20Y-B | presence of 1% (w/v) AMPHITOL 20Y-B causes strong inhibitions, resulting in an enzyme retaining only 50% of its activity | Thermococcus kodakarensis | |
EDTA | about 20% loss of activity in the presence of 0.01% (w/v) at 80°C, about 60% loss of activity in the presence of 1% (w/v) at 80°C; activity decreases with the increasing concentration of EDTA from 0.01 to 1% (w/v) | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | P58502 | sequence including singnal peptide (amino acid 1-24) and propeptide (amino acid 25-106) | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
abnormal prion protein PrP(Sc) + H2O | - |
Thermococcus kodakarensis | ? | - |
? | |
abnormal prion protein PrP(Sc) + H2O | prion protein PrP(Sc) is a pathological prion protein PrP isoform. The enzyme can disrupt PrPSc to a level undetectable by Western-blot analysis | Thermococcus kodakarensis | ? | - |
? | |
azocasein + H2O | - |
Thermococcus kodakarensis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Tk-SP | - |
Thermococcus kodakarensis |
Tk-subtilisin | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Thermococcus kodakarensis |