Literature summary for 3.4.21.B57 extracted from

Tanaka, S.; Saito, K.; Chon, H.; Matsumura, H.; Koga, Y.; Takano, K.; Kanaya, S.
Crystallization and preliminary X-ray diffraction study of an active-site mutant of pro-Tk-subtilisin from a hyperthermophilic archaeon (2006), Acta Crystallogr. Sect. F, 62, 902-905.

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Cloned(Commentary)

Cloned (Comment)	Organism
overproduction of pro-S255A in Escherichia coli BL21-Codon-Plus(DE3)	Thermococcus kodakarensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystallization of the active-site mutant S255A of pro-Tk-subtilisin. The crystal is grown at 4°C by the sitting-drop vapour-diffusion method. Native X-ray diffraction data are collected to 2.3 A resolution.They crystal belongs to the orthorhombic space group I222, with unit-cell parameters a = 92.69, b = 121.78, c = 77.53 A. Assuming the presence of one molecule per asymmetric unit, the Matthews coefficient V(M) was calculated to be 2.6 A(3) Da(-1) and the solvent content was 53.1%	Thermococcus kodakarensis

Crystallization (Comment)

Organism

crystallization of the active-site mutant S255A of pro-Tk-subtilisin. The crystal is grown at 4°C by the sitting-drop vapour-diffusion method. Native X-ray diffraction data are collected to 2.3 A resolution.They crystal belongs to the orthorhombic space group I222, with unit-cell parameters a = 92.69, b = 121.78, c = 77.53 A. Assuming the presence of one molecule per asymmetric unit, the Matthews coefficient V(M) was calculated to be 2.6 A(3) Da(-1) and the solvent content was 53.1%

Thermococcus kodakarensis

Protein Variants

Protein Variants	Comment	Organism
S255A	active-site mutant enzyme	Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	active-site mutant S255A of pro-Tk-subtilisin	Thermococcus kodakarensis

Organism

Organism	UniProt	Comment	Textmining
Thermococcus kodakarensis	P58502	sequence including singnal peptide (amino acid 1-24) and propeptide (amino acid 25-106)	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermococcus kodakarensis

Subunits

Subunits	Comment	Organism
monomer	1 * 45000, active-site mutant S255A of pro-Tk-subtilisin, SDS-PAGE	Thermococcus kodakarensis

Synonyms

Synonyms	Comment	Organism
Tk-subtilisin	-	Thermococcus kodakarensis