Cloned (Comment) | Organism |
---|---|
the recombinant proform of Pyrococcus furiosus pyrolysin and several N- and C-terminal deletion mutants are successfully expressed in Escherichia coli | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.32 | - |
succinyl-Ala-Ala-Pro-Lys-4-nitroanilide | pH 7.5, 90°C, mature enzyme | Pyrococcus furiosus | |
3.83 | - |
succinyl-Ala-Ala-Pro-Lys-4-nitroanilide | pH 7.5, 90°C, mature enzyme lacking the C-terminal extension (mPlsDELTAC740) | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | P72186 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the maturation of the enzyme involves autoprocessing of both the N-propeptide and a C-terminal propeptide. The long C-terminal extension can be dissected into a C-terminal propeptide and a C-terminal extension (CTEm) that is attached to the catalytic domain of the mature enzyme. Both of these propeptides assist in achieving pyrolysin hyperthermostability | Pyrococcus furiosus |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
succinyl-Ala-Ala-Pro-Lys-4-nitroanilide + H2O | - |
Pyrococcus furiosus | succinyl-Ala-Ala-Pro-Lys + 4-nitroaniline | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
assay at | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1893 | - |
succinyl-Ala-Ala-Pro-Lys-4-nitroanilide | pH 7.5, 90°C, mature enzyme lacking the C-terminal extension (mPlsDELTAC740) | Pyrococcus furiosus | |
3128 | - |
succinyl-Ala-Ala-Pro-Lys-4-nitroanilide | pH 7.5, 90°C, mature enzyme | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
494 | - |
succinyl-Ala-Ala-Pro-Lys-4-nitroanilide | pH 7.5, 90°C, mature enzyme lacking the C-terminal extension (mPlsDELTAC740) | Pyrococcus furiosus | |
942 | - |
succinyl-Ala-Ala-Pro-Lys-4-nitroanilide | pH 7.5, 90°C, mature enzyme | Pyrococcus furiosus |