Literature summary for 3.4.21.B50 extracted from

Schubert, A.; Wrase, R.; Hilgenfeld, R.; Hansen, G.
Structures of DegQ from Legionella pneumophila define distinct on and off states (2015), J. Mol. Biol., 427, 2840-2851.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting-drop vapor diffusion technique. The best crystals are obtained when using 100 mM citrate (pH 5.5) and 17.5% polyethylene glycol 400 or 100 mM sodium acetate (pH 4.5), 50 mM CdCl2 and 28% polyethylene glycol 400 as reservoir solutions. Crystal structures of inactive 3-mers and active 12-mers of DegQ reveal molecular details of elements of a conserved allosteric activation cascade that defines distinct protease ON and OFF states	Legionella pneumophila subsp. pneumophila

Crystallization (Comment)

Organism

sitting-drop vapor diffusion technique. The best crystals are obtained when using 100 mM citrate (pH 5.5) and 17.5% polyethylene glycol 400 or 100 mM sodium acetate (pH 4.5), 50 mM CdCl2 and 28% polyethylene glycol 400 as reservoir solutions. Crystal structures of inactive 3-mers and active 12-mers of DegQ reveal molecular details of elements of a conserved allosteric activation cascade that defines distinct protease ON and OFF states

Legionella pneumophila subsp. pneumophila

Organism

Organism	UniProt	Comment	Textmining
Legionella pneumophila subsp. pneumophila	Q5ZVV9	-	-
Legionella pneumophila subsp. pneumophila ATCC 33152	Q5ZVV9	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DPMFKLV-4-methylcoumaryl-7-amide + H2O	-	Legionella pneumophila subsp. pneumophila	?	-	?
DPMFKLV-4-methylcoumaryl-7-amide + H2O	-	Legionella pneumophila subsp. pneumophila ATCC 33152	?	-	?

Subunits

Subunits	Comment	Organism
additional information	DegQLp forms predominantly trimers in the substrate-free state. In vitro, oligomerization is influenced by the pH of the protein solution and the presence of co-purified peptides binding to the PDZ1 and/or protease site. Misfolded proteins or peptides promote the assembly of protease active 12- or 24-mers the assembly of proteolytically active	Legionella pneumophila subsp. pneumophila

Synonyms

Synonyms	Comment	Organism
DegQ	-	Legionella pneumophila subsp. pneumophila

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Legionella pneumophila subsp. pneumophila

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Legionella pneumophila subsp. pneumophila