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Literature summary for 3.4.21.B48 extracted from

  • Murao, S.; Ohkuni, K.; Nagao, M.; Hirayama, K.; Fukuhara, K.; Oda, K.; Oyama, H.; Shin, T.
    Purification and characterization of kumamolysin, a novel thermostable pepstatin-insensitive carboxyl proteinase from Bacillus novosp. MN-32 (1993), J. Biol. Chem., 268, 349-355.
    View publication on PubMed
Search for more literature for 3.4.21.B48

Inhibitors

Inhibitors Comment Organism Structure
additional information insensitive to pepstatin, diazoacetyl-DL-norleucine methyl ester, and 1,2-epoxy-3-(p-nitrophenoxy)propane Bacillus novosp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 oxidized insulin B-chain pH 3.0, 30°C Bacillus novosp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Bacillus novosp.
41000
-
 1 * 41000, SDS-PAGE Bacillus novosp.

Organism

Organism UniProt Comment Textmining
Bacillus novosp.
-
 MN-32
-
Bacillus novosp. MN-32
-
 MN-32
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
 Bacillus novosp.

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus novosp.

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Bacillus novosp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus novosp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Bacillus novosp. ?
-
 ?
casein + H2O
-
 Bacillus novosp. MN-32 ?
-
 ?
Oxidized insulin B-chain + H2O specifically hydrolyzes Leu15-Tyr16 peptide bond in oxidized insulin B-chain, additional cleavage at Phe25-Tyr26 at a considerably lower rate Bacillus novosp. ?
-
 ?
Oxidized insulin B-chain + H2O specifically hydrolyzes Leu15-Tyr16 peptide bond in oxidized insulin B-chain, additional cleavage at Phe25-Tyr26 at a considerably lower rate Bacillus novosp. MN-32 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Bacillus novosp.

Synonyms

Synonyms Comment Organism
kumamolisin
-
 Bacillus novosp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Bacillus novosp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 80 50°C: about 25% of maximal activity, 80°C: about 60% of maximal activity Bacillus novosp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 10 min, stable below Bacillus novosp.
80
-
 10 min, enzyme retains 60% of its original activity Bacillus novosp.
90
-
 10 min, complete loss of activity Bacillus novosp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
71
-
 oxidized insulin B-chain pH 3.0, 30°C Bacillus novosp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 hydrolysis of casein Bacillus novosp.

pH Range

pH Minimum pH Maximum Comment Organism
2.5 4 pH 2.5: about 60% of maximal activity, pH 4.0: about 50% of maximal activity, hydrolysis of casein Bacillus novosp.

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus novosp. isoelectric focusing, carrier ampholine pH 3-10
-
 3.5