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Literature summary for 3.4.21.B48 extracted from
- Subsite preferences of pepstatin-insensitive carboxyl proteinases from prokaryotes: kumamolysin, a thermostable pepstatin-insensitive carboxyl proteinase (2000), J. Biochem., 128, 499-507.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | insensitive to pepstatin, diazoacetyl-DL-norleucine methylester, and 1,2-epoxy-3-(p-nitro-phenoxy)propane | Bacillus novosp. |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0122 | - |
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Arg | - |
Bacillus novosp. |  |
| 0.0128 | - |
Lys-Arg-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0128 | - |
Lys-Pro-Ile-Ile-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0154 | - |
Lys-Pro-Ala-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0211 | - |
Ser-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0246 | - |
Lys-Pro-Lys-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0248 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Tyr | - |
Bacillus novosp. | |
| 0.0269 | - |
Leu-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0285 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ile-Leu | - |
Bacillus novosp. | |
| 0.0349 | - |
Lys-Asp-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0351 | - |
Lys-Pro-Asp-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0371 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ala-Leu | - |
Bacillus novosp. | |
| 0.0376 | - |
Lys-Pro-Gln-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0377 | - |
Lys-Pro-Asn-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0377 | - |
Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0382 | - |
Ala-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0389 | - |
Lys-Pro-Phe-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0404 | - |
Lys-Pro-Glu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0405 | - |
Lys-Pro-Ile-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0406 | - |
Lys-Pro-Ala-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0409 | - |
Lys-Pro-Ile-Pro-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.041 | - |
Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0426 | - |
Lys-Pro-Ile-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0428 | - |
Lys-Leu-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0438 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Glu-Leu | - |
Bacillus novosp. | |
| 0.0447 | - |
Lys-Pro-Ile-Thr-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0468 | - |
Lys-Pro-Ile-Nle-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0469 | - |
Lys-Pro-Thr-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0473 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ser-Leu | - |
Bacillus novosp. | |
| 0.0517 | - |
Lys-Ala-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0527 | - |
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ser | - |
Bacillus novosp. | |
| 0.056 | - |
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0567 | - |
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ala | - |
Bacillus novosp. | |
| 0.0575 | - |
Lys-Pro-Leu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0577 | - |
Lys-Pro-Ser-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0589 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Val | - |
Bacillus novosp. | |
| 0.0603 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0634 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Phe | - |
Bacillus novosp. | |
| 0.0636 | - |
Lys-Pro-Ala-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0642 | - |
Lys-Pro-Ile-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0645 | - |
Lys-Pro-Ala-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0654 | - |
Lys-Pro-Asp-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0671 | - |
Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0674 | - |
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Asp | - |
Bacillus novosp. | |
| 0.0697 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ile | - |
Bacillus novosp. | |
| 0.073 | - |
Lys-Pro-Ile-Asn-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0767 | - |
Lys-Pro-Ile-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0825 | - |
Lys-Pro-Ile-Val-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0831 | - |
Lys-Pro-Ile-His-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.0883 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Val-Leu | - |
Bacillus novosp. | |
| 0.0995 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ala | - |
Bacillus novosp. | |
| 0.1 | - |
Lys-Pro-Ser-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.103 | - |
Arg-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.105 | - |
Lys-Pro-Val-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.109 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Leu-Leu | - |
Bacillus novosp. | |
| 0.109 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Asp | - |
Bacillus novosp. | |
| 0.119 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Nle | - |
Bacillus novosp. | |
| 0.119 | - |
Lys-Pro-Arg-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.128 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Lys-Leu | - |
Bacillus novosp. | |
| 0.129 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ser | - |
Bacillus novosp. | |
| 0.133 | - |
Lys-Pro-Ala-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.142 | - |
Lys-Pro-Arg-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.154 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Arg | - |
Bacillus novosp. | |
| 0.162 | - |
Lys-Ser-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.225 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asp-Leu | - |
Bacillus novosp. | |
| 0.43 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asn-Leu | - |
Bacillus novosp. | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus novosp. | - |
MN-32 | - |
| Bacillus novosp. MN-32 | - |
MN-32 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Ala-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Ala-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. MN-32 | Ala-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Arg-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Arg-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Leu-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Leu-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Leu-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. MN-32 | Leu-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Ala-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Ala-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Arg-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Arg-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Asp-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Asp-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Leu-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Leu-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ala-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ala-Ala-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ala-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ala-Arg-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ala-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ala-Asp-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ala-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ala-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ala + H2O | - |
Bacillus novosp. | Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Ala | - |
? | |
| Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Arg + H2O | - |
Bacillus novosp. | Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Arg | - |
? | |
| Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Asp + H2O | - |
Bacillus novosp. | Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Asp | - |
? | |
| Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. MN-32 | Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ser + H2O | - |
Bacillus novosp. | Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Ser | - |
? | |
| Lys-Pro-Ala-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ala-Ser-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Arg-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Arg-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Arg-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Arg-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Asn-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Asn-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Asp-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Asp-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Asp-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Asp-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Gln-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Gln-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Glu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Glu-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ile-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Ala-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ile-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Arg-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ile-Asn-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Asn-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ile-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Asp-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Gln-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ala-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Ala-Leu | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ala + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Ala | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Arg + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Arg | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Asp + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Asp | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ile + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Ile | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Nle + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Nle | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Phe + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Phe | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ser + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Ser | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Tyr + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Tyr | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Val + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Val | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asn-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Asn-Leu | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asp-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Asp-Leu | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Glu-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Glu-Leu | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ile-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Ile-Leu | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Leu-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Leu-Leu | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Lys-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Lys-Leu | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ser-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Ser-Leu | - |
? | |
| Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Val-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Val-Leu | - |
? | |
| Lys-Pro-Ile-His-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-His-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ile-Ile-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Ile-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Leu-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ile-Nle-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Nle-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ile-Pro-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Pro-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ile-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Ser-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ile-Thr-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Thr-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ile-Val-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Val-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Leu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Leu-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Lys-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Lys-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Phe-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Phe-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ser-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ser-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Ser-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ser-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Thr-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Thr-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Pro-Val-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Val-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Lys-Ser-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Ser-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| additional information | the enzyme preferentially hydrolyzes peptides having an Ala or Pro residue at P2 position and prefers such charged amino acid residues as Glu or Arg at the P2' position. No cleavage: Asp-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | Bacillus novosp. | ? | - |
? | |
| additional information | the enzyme preferentially hydrolyzes peptides having an Ala or Pro residue at P2 position and prefers such charged amino acid residues as Glu or Arg at the P2' position. No cleavage: Asp-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | Bacillus novosp. MN-32 | ? | - |
? | |
| Ser-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. | Ser-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
| Ser-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O | - |
Bacillus novosp. MN-32 | Ser-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | 1.97 | Lys-Pro-Ile-Asn-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.04 | 1.97 | Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asp-Leu | - |
Bacillus novosp. | |
| 0.052 | 2.1 | Lys-Pro-Ser-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.19 | - |
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Asp | - |
Bacillus novosp. | |
| 0.22 | - |
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ala | - |
Bacillus novosp. | |
| 0.26 | - |
Leu-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.27 | - |
Ser-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.3 | - |
Lys-Pro-Ile-Ile-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.41 | - |
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.45 | - |
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ser | - |
Bacillus novosp. | |
| 0.54 | - |
Ala-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.59 | - |
Lys-Pro-Ser-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.64 | - |
Lys-Pro-Asp-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.73 | - |
Lys-Pro-Ile-Val-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.84 | - |
Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 0.98 | - |
Lys-Pro-Ala-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 1.04 | - |
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Arg | - |
Bacillus novosp. | |
| 1.13 | - |
Lys-Pro-Arg-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 1.46 | - |
Lys-Asp-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 1.82 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ile-Leu | - |
Bacillus novosp. | |
| 2.35 | - |
Lys-Ser-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 2.6 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ser-Leu | - |
Bacillus novosp. | |
| 2.94 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ile-Leu | - |
Bacillus novosp. | |
| 3 | 6 | Lys-Pro-Asp-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 3 | 6 | Lys-Pro-Ile-Thr-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 3 | 6 | Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asn-Leu | - |
Bacillus novosp. | |
| 3 | - |
Lys-Pro-Ile-His-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 3.12 | - |
Lys-Pro-Asp-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 3.13 | - |
Lys-Pro-Phe-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 3.13 | - |
Lys-Pro-Leu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 3.3 | - |
Lys-Pro-Asn-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 3.3 | - |
Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 3.3 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Val-Leu | - |
Bacillus novosp. | |
| 3.48 | - |
Lys-Pro-Ile-Thr-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 3.48 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asn-Leu | - |
Bacillus novosp. | |
| 3.5 | - |
Lys-Pro-Ala-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 3.7 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Phe | - |
Bacillus novosp. | |
| 3.76 | - |
Lys-Pro-Phe-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 4.44 | - |
Lys-Pro-Leu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 4.62 | - |
Lys-Pro-Asn-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 4.79 | - |
Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 5.04 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Val-Leu | - |
Bacillus novosp. | |
| 6.08 | - |
Ala-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 6.08 | - |
Lys-Asp-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 6.08 | - |
Lys-Pro-Asp-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 6.08 | - |
Lys-Pro-Ser-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 6.08 | - |
Lys-Pro-Arg-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 6.08 | - |
Lys-Pro-Ala-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 6.08 | - |
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Arg | - |
Bacillus novosp. | |
| 6.08 | - |
Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 6.08 | - |
Lys-Pro-Ile-Val-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 6.08 | - |
Lys-Arg-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 6.5 | - |
Lys-Ala-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 7.7 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ala-Leu | - |
Bacillus novosp. | |
| 8.78 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asp-Leu | - |
Bacillus novosp. | |
| 9.14 | - |
Lys-Pro-Ile-Asn-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 10.9 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Lys-Leu | - |
Bacillus novosp. | |
| 12.5 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ile | - |
Bacillus novosp. | |
| 13.1 | - |
Lys-Pro-Glu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 13.1 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Val | - |
Bacillus novosp. | |
| 15.9 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Tyr | - |
Bacillus novosp. | |
| 16.1 | - |
Lys-Pro-Gln-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 16.7 | - |
Lys-Pro-Thr-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 18.6 | - |
Lys-Pro-Val-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 19.9 | - |
Lys-Pro-Ala-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 20.3 | - |
Lys-Leu-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 21.7 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Asp | - |
Bacillus novosp. | |
| 22.7 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Glu-Leu | - |
Bacillus novosp. | |
| 23.9 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Leu-Leu | - |
Bacillus novosp. | |
| 25.4 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 28.6 | - |
Lys-Pro-Ile-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 31.9 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Phe | - |
Bacillus novosp. | |
| 32.3 | - |
Lys-Pro-Arg-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 34.2 | - |
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Nle | - |
Bacillus novosp. | |
| 48.5 | - |
Lys-Pro-Ile-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 52.7 | - |
Lys-Pro-Ile-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 81 | - |
Lys-Pro-Ile-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 98.3 | - |
Lys-Pro-Ala-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
| 176 | - |
Lys-Pro-Ile-Pro-Phe-(p-nitrophenylalanine)-Arg-Leu | - |
Bacillus novosp. | |
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