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Literature summary for 3.4.21.B43 extracted from

  • Kryza, T.; Achard, C.; Parent, C.; Marchand-Adam, S.; Guillon-Munos, A.; Iochmann, S.; Korkmaz, B.; Respaud, R.; Courty, Y.; Heuze-Vourch, N.
    Angiogenesis stimulated by human kallikrein-related peptidase 12 acting via a platelet-derived growth factor B-dependent paracrine pathway (2014), FASEB J., 28, 740-751.
    View publication on PubMed
Search for more literature for 3.4.21.B43

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information enzyme reaction kinetics, overview Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
platelet-derived growth factor B precursor + H2O Homo sapiens membrane bound zymogen mature platelet-derived growth factor B + ? soluble mature paracrine factor ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UKR0
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification activation of the proform of the enzyme, pro-KLK12 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
BEAS-2B cell
-
 Homo sapiens
-
endothelial cell
-
 Homo sapiens
-
epithelial cell
-
 Homo sapiens
-
fibroblast
-
 Homo sapiens
-
lung
-
 Homo sapiens
-
additional information KLK12 is a trypsin-like kallikrein-related peptidase that is abundant in a variety of human tissues Homo sapiens
-
NCI-H1838 cell
-
 Homo sapiens
-
NCI-H23 cell
-
 Homo sapiens
-
NCI-H460 cell
-
 Homo sapiens
-
NCI-H520 cell
-
 Homo sapiens
-
NCI-H522 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-VPR-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Boc-VPR + 7-amino-4-methylcoumarin
-
 ?
platelet-derived growth factor B precursor + H2O membrane bound zymogen Homo sapiens mature platelet-derived growth factor B + ? soluble mature paracrine factor ?
platelet-derived growth factor B precursor + H2O membrane bound zymogen Homo sapiens mature platelet-derived growth factor B + ? soluble mature protein ?

Synonyms

Synonyms Comment Organism
kallikrein-related peptidase 12
-
 Homo sapiens
KLK12
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Expression

Organism Comment Expression
Homo sapiens Expression of both KLK12 and PDGFB genes is regulated by hypoxia in the lung tumor microenvironment additional information

General Information

General Information Comment Organism
metabolism PDGF-B is probably one of the molecules linking the interaction between enzyme-induced endothelial cells and fibroblasts. Expression of both KLK12 and PDGFB genes is regulated by hypoxia in the lung tumor microenvironment Homo sapiens
additional information KLK12 is a trypsin-like kallikrein-related peptidase that is abundant in a variety of human tissues Homo sapiens
physiological function the enzyme induces the formation of pulmonary endothelial cell tubule-like structures. The enzyme converts the extracellular matrix- or membrane-bound precursor of platelet-derived growth factor B (PDGF-B) into a soluble active form. Both PDGF-B and vascular endothelial growth factor A (VEGF-A) take part in the induction of angiogenesis by enzyme KLK12 in a coculture model of angiogenesis that mimics endothelial tubule formation. Release of mature PDGF-B by enzyme KLK12 leads to the fibroblast-mediated secretion of VEGF-A, which stimulates endothelial cell differentiation and the formation of capillary tube-like structures. PDGF-B signaling ultimately results in cell growth, migration, and protection against apoptosis, overview Homo sapiens