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Literature summary for 3.4.21.B39 extracted from

  • Michael, I.P.; Sotiropoulou, G.; Pampalakis, G.; Magklara, A.; Ghosh, M.; Wasney, G.; Diamandis, E.P.
    Biochemical and enzymatic characterization of human kallikrein 5 (hK5), a novel serine protease potentially involved in cancer progression (2005), J. Biol. Chem., 280, 14628-14635.
    View publication on PubMed
Search for more literature for 3.4.21.B39

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
alpha2-antiplasmin 50% inhibition at 0.8 microM in 0.6 min Homo sapiens
antithrombin III 50% inhibition at 0.8 microM in 6 min Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 Boc-Val-Pro-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
0.15
-
 Boc-Leu-GLy-Arg-7-amido-4-methylcoumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
0.19
-
 Boc-Phe-Ser-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
0.31
-
 Z-Gly-Gly-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
0.48
-
 Boc-Leu-Arg-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
0.57
-
 Boc-Gln-Gly-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
0.61
-
 Boc-Gln-Ala-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
0.64
-
 Boc-Val-Leu-Lys-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
1.01
-
 Boc-Leu-Lys-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
1.41
-
 Boc-Gln-Arg-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
1.69
-
 Tos-Gly-Pro-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
1.77
-
 H-Pro-Phe-Ser-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 calculated from sequence Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
two chromatography steps, 95% purity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-Gln-Ala-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Boc-Gln-Ala-Arg + 7-amino-4-methyl-coumarin
-
 ?
Boc-Gln-Arg-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Boc-Gln-Arg-Arg + 7-amino-4-methyl-coumarin
-
 ?
Boc-Gln-Gly-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Boc-Gln-Gly-Arg + 7-amino-4-methyl-coumarin
-
 ?
Boc-Leu-Arg-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Boc-Leu-Arg-Arg + 7-amino-4-methyl-coumarin
-
 ?
Boc-Leu-GLy-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Boc-Leu-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
Boc-Leu-Lys-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Boc-Leu-Lys-Arg + 7-amino-4-methyl-coumarin
-
 ?
Boc-Phe-Ser-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Boc-Phe-Ser-Arg + 7-amino-4-methyl-coumarin
-
 ?
Boc-Val-Leu-Lys-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Boc-Val-Leu-Lys + 7-amino-4-methyl-coumarin
-
 ?
Boc-Val-Pro-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Boc-Val-Pro-Arg + 7-amino-4-methyl-coumarin
-
 ?
casein + H2O
-
 Homo sapiens ?
-
 ?
fibrinogen Aalpha chain + H2O cleavage after Arg348 and Arg407 Homo sapiens ?
-
 ?
fibrinogen Bbeta chain + H2O cleavage after Lys53 and Arg14 Homo sapiens ?
-
 ?
Gelatin + H2O
-
 Homo sapiens ?
-
 ?
H-Pro-Phe-Ser-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens H-Pro-Phe-Ser-Arg + 7-amino-4-methyl-coumarin
-
 ?
kininogen + H2O cleavage after Arg222, Arg252 and Arg374 Homo sapiens ?
-
 ?
plasminogen + H2O cleavage after Lys77 andA rg529 Homo sapiens ?
-
 ?
Pro-Phe-Ser-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Pro-Phe-Ser-Arg + 7-amino-4-methyl-coumarin
-
 ?
Tos-Gly-Pro-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Tos-Gly-Pro-Arg + 7-amino-4-methyl-coumarin
-
 ?
Vitronectin + H2O cleavage after Arg8 Homo sapiens ?
-
 ?
Z-Gly-Gly-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Z-Gly-Gly-Arg + 7-amino-4-methyl-coumarin
-
 ?

Synonyms

Synonyms Comment Organism
hK5
-
 Homo sapiens
human kallikrein 5
-
 Homo sapiens
KLK5
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 Z-Gly-Gly-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
0.07
-
 Boc-Leu-GLy-Arg-7-amido-4-methylcoumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
0.08
-
 Boc-Val-Leu-Lys-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
0.18
-
 Boc-Gln-Gly-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
0.31
-
 Boc-Leu-Arg-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
0.35
-
 Tos-Gly-Pro-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
0.41
-
 Boc-Gln-Arg-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
0.47
-
 H-Pro-Phe-Ser-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
0.82
-
 Boc-Leu-Lys-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
1.78
-
 Boc-Gln-Ala-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
2.83
-
 Boc-Phe-Ser-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.28
-
 Boc-Val-Pro-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00107
-
 alpha2-antiplasmin
-
 Homo sapiens
0.0091
-
 antithrombin III
-
 Homo sapiens