Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.B39 extracted from

  • Brattsand, M.; Egelrud, T.
    Purification, molecular cloning, and expression of a human stratum corneum trypsin-like serine protease with possible function in desquamation (1999), J. Biol. Chem., 274, 30033-30040.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned from a keratinocyte library, DNA sequence determination and analysis, expression in CHO-K1 cells via infection with Semliki Forest Virus system Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
about, native and recombinant enzyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polypeptide + H2O Homo sapiens
-
peptides
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9Y337 can be processed to active enzyme from inactive precursor by trypsin
-
Homo sapiens Q9Y337 gene KLK5 or SCTE
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylated Homo sapiens
proteolytic modification can be processed to active enzyme from inactive precursor by trypsin Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
from epidermis Homo sapiens
recombinant from CHO-K1 cells Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
proteolytic cleavage of polypeptides enzyme is a serine protease Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-
epidermis
-
Homo sapiens
-
epithelium of plantar skin Homo sapiens
-
keratinocyte
-
Homo sapiens
-
kidney
-
Homo sapiens
-
placenta
-
Homo sapiens
-
skin
-
Homo sapiens
-
stratum corneum
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polypeptide + H2O
-
Homo sapiens peptides
-
?

Subunits

Subunits Comment Organism
monomer 1 * 30000-37000, recombinant enzyme, SDS-PAGE Homo sapiens
monomer 1 * 25000-33000, native enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
strateum-corneum trypsin-like serine protease
-
Homo sapiens