Crystallization (Comment) | Organism |
---|---|
enzyme in complex with inhibitor: decanoyl-Arg-Val-Arg-Lys-chloromethyl ketone, Asp-Gln-Thr-Gln-Lys-Gln-Tyr-Gln-Glu-Leu-Thr-Phe-Phe-chloromethyl ketone or benzyloxycarbonyl-Phe-PSI[CO-CONH]Arg-Glu-Phe-OH, hanging drop vapour diffusion method | Thermoplasma acidophilum |
Protein Variants | Comment | Organism |
---|---|---|
L184C | increase in activity with benzoyl-Val-Gly-Arg-7-amido-4-methylcoumarin and insulin B chain to more than 150% of the wild-type activity, no activity with casein | Thermoplasma acidophilum |
R131E/R132E | slight decrease in activity with benzoyl-Val-Gly-Arg-7-amido-4-methylcoumarin, strong decrease in activity with insulin B chain, barely detectable activity with casein | Thermoplasma acidophilum |
R414C/A643C | decrease in activity with benzoyl-Val-Gly-Arg-7-amido-4-methylcoumarin to 70% of wild-type activity, decrease in activity with insulin B chain as substrate to 50% of wild-type activity, no activity with casein | Thermoplasma acidophilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Asp-Gln-Thr-Gln-Lys-Gln-Tyr-Gln-Glu-Leu-Thr-Phe-Phe-chloromethyl ketone | - |
Thermoplasma acidophilum | |
benzyloxycarbonyl-Phe-PSI[CO-CONH]Arg-Glu-Phe-OH | - |
Thermoplasma acidophilum | |
decanoyl-Arg-Val-Arg-Lys-chloromethyl ketone | - |
Thermoplasma acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | P96086 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-Val-Gly-Arg-7-amido-4-methylcoumarin + H2O | - |
Thermoplasma acidophilum | benzoyl-Val-Gly-Arg + 7-amino-4-methylcoumarin | - |
? | |
casein + H2O | - |
Thermoplasma acidophilum | ? | - |
? | |
insulin B chain + H2O | - |
Thermoplasma acidophilum | ? | - |
? |