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Literature summary for 3.4.21.B3 extracted from

  • Gladysheva, I.P.; Popykina, N.A.; Zamolodchikova, T.S.; Larionova, N.I.
    Interaction between duodenase and alpha1-proteinase inhibitor (2001), Biochemistry (Moscow), 66, 682-687.
    View publication on PubMed
Search for more literature for 3.4.21.B3

Inhibitors

Inhibitors Comment Organism Structure
Bowman Birk soybean inhibitor
-
 Bos taurus
Human alpha1-proteinase inhibitor suicide mechanism, regulatory effect Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
 alpha-N-tosyl-Gly-Pro-Lys-4-nitroanilide pH 8.0, 25°C Bos taurus
0.56
-
 alpha-N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 8.0, 25°C Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polypeptide + H2O Bos taurus
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus P80219
-
-

Reaction

Reaction Comment Organism Reaction ID
proteolytic cleavage of polypeptides to large and stable peptides, preferential cleavage sites: Lys-, Arg-, Tyr-, Phe-, Leu-, -Pro serine protease which has a dual specificity, i.e. both trypsin-like and chymotrypsin-like activities Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
duodenum
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Bos taurus alpha-N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
alpha-N-tosyl-Gly-Pro-Lys-4-nitroanilide + H2O
-
 Bos taurus alpha-N-tosyl-Gly-Pro-Lys + 4-nitroaniline
-
 ?
polypeptide + H2O
-
 Bos taurus peptides
-
 ?

Synonyms

Synonyms Comment Organism
duodenase I
-
 Bos taurus
duodenase serine protease
-
 Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9
-
 alpha-N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 8.0, 25°C Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000013
-
 Human alpha1-proteinase inhibitor pH 8.0, 25°C Bos taurus