Literature summary for 3.4.21.B28 extracted from

Aertgeerts, K.; Levin, I.; Shi, L.; Snell, G.P.; Jennings, A.; Prasad, G.S.; Zhang, Y.; Kraus, M.L.; Salakian, S.; Sridhar, V.; Wijnands, R.; Tennant, M.G.
Structural and kinetic analysis of the substrate specificity of human fibroblast activation protein alpha (2005), J. Biol. Chem., 280, 19441-19444.

Search for more literature for 3.4.21.B28

Cloned(Commentary)

Cloned (Comment)	Organism
into a modified pFastBacHTb vector	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
at 4°C, by Syrrx´s automated Nanovolume crystallization technology, Ala657 reduces the acidity in this pocket, which may explain the lower affinity for N-terminal amines	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
A657D	shows a 60fold increase in the catalytic efficiency for the cleavage of dipeptide substrates, catalytic efficiency reduced by ca. 350fold for the cleavage of benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
90000	-	theoretical value of FAPalpha-His6tag	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
standard protocol	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O	-	Homo sapiens	Ala-Pro + 7-amino-4-trifluoromethylcoumarin	-	?
benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin + H2O	-	Homo sapiens	benzyloxycarbonyl-Gly-Pro + 7-amino-4-methylcoumarin	-	?
Gly-Pro-7-amido-4-methylcoumarin + H2O	-	Homo sapiens	Gly-Pro + 7-amino-4-methylcoumarin	-	?
Gly-Pro-7-amido-4-trifluoromethylcoumarin + H2O	-	Homo sapiens	Gly-Pro + 7-amino-4-trifluoromethylcoumarin	-	?

Subunits

Subunits	Comment	Organism
homodimer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
FAPalpha	-	Homo sapiens

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Release 2023.1 (January 2023)