Literature summary for 3.4.21.B28 extracted from

Sun, S.; Albright, C.F.; Fish, B.H.; George, H.J.; Selling, B.H.; Hollis, G.F.; Wynn, R.
Expression, purification, and kinetic characterization of full-length human fibroblast activation protein (2002), Protein Expr. Purif., 24, 274-281.

Search for more literature for 3.4.21.B28

Inhibitors

Inhibitors	Comment	Organism	Structure
2-mercaptoethanol	inhibition at 140 mM	Homo sapiens
chymostatin	50% inhibition at 0.16 mM	Homo sapiens
dimethyl sulfoxide	50% inhibition at 4%	Homo sapiens
dithiothreitol	inhibition at 10 mM	Homo sapiens
phenylmethylsulfonyl fluoride	more than 50% inhibition at 1 mM	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2	-	Ala-Pro-4-trifluoromethylcoumarin-7-amide	pH 8.5	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q12884	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	two isoforms: glycosylated and nonglycosylated	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
glycosylated form, near homogeneity	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Ala-Pro-4-trifluoromethylcoumarin-7-amide + H2O	-	Homo sapiens	Ala-Pro + 7-amino-4-trifluoromethylcoumarin	-	?
additional information	glycosylated form has both postprolyl depeptidyl peptidase and postgelatinase activity, nonglycosylated isoform has no detectable gelatinase activity	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
FAP	-	Homo sapiens
fibroblast activation factor	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2	-	Ala-Pro-4-trifluoromethylcoumarin-7-amide	pH 8.5	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	9	-	Homo sapiens

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Release 2023.1 (January 2023)