Literature summary for 3.4.21.B28 extracted from

  • Park, J.E.; Lenter, M.C.; Zimmermann, R.N.; Garin-Chesa, P.; Old, L.J.; Rettig, W.J.
    Fibroblast activation protein, a dual specificity serine protease expressed in reactive human tumor stromal fibroblasts (1999), J. Biol. Chem., 274, 36505-36512.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Engineering

Protein Variants Comment Organism
S624A inactive mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
diisopropylfluorophosphate 50% inhibition at 0.02 mM Homo sapiens
phenylmethylsulfonyl fluoride 50% inhibition at 0.1 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
Lys-Pro-4-trifluoromethylcoumarin-7-amide pH 7.8, 37°C Homo sapiens
0.46
-
Ala-Pro-4-trifluoromethylcoumarin-7-amide pH 7.8, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
215000
-
SDS-PAGE under nonreducing conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Type I collagen + H2O Homo sapiens
-
?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast reactive tumor stromal fibroblast and granulation tissue during wound healing Homo sapiens
-
additional information no activity in normal adult human tissues Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gelatin + H2O
-
Homo sapiens ?
-
?
Type I collagen + H2O
-
Homo sapiens ?
-
?
Ala-Pro-4-trifluoromethylcoumarin-7-amide + H2O
-
Homo sapiens Ala-Pro + 7-amino-4-trifluoromethylcoumarin
-
?
Lys-Pro-4-trifluoromethylcoumarin-7-amide + H2O
-
Homo sapiens Lys-Pro + 7-amino-4-trifluoromethylcoumarin
-
?

Subunits

Subunits Comment Organism
dimer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
seprase
-
Homo sapiens
FAP
-
Homo sapiens