BRENDA - Enzyme Database show
show all sequences of 3.4.21.B26

Endoproteolytic activation of av integrin by proprotein convertase PC5 is required for Vascular smooth muscle cell adhesion to vitronectin and integrin-dependent signaling

Stawowy, P.; Kallisch, H.; Veinot, J.P.; Kilimnik, A.; Prichett, W.; Goetze, S.; Seidah, N.G.; Chretien, M.; Fleck, E.; Graf, K.; Circulation 109, 770-776 (2004)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
carotid atherosclerotic lesions obtained by endaterectomy contain PC5, PC5 colocalizes with av integrin
Homo sapiens
medicine
av endoproteolytic activation is necessary for integrin-mediated adhesion and migration as well as signaling and requires PC5 in vascular smooth muscle cells
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
decanoyl-Arg-Val-Lys-Arg-chloromethylketone
0.05 mM inhibits PC activity and consequently av cleavage
Rattus norvegicus
additional information
av cleavage not inhibited by the MMP-inhibitor GM6001 and PC5-specific antisense oligonucleotide-3
Rattus norvegicus
PC5-specific antisense oligonucleotide-1
inhibits PC activity and consequently av cleavage
Rattus norvegicus
PC5-specific antisense oligonucleotide-2
inhibits PC activity and consequently av cleavage, most potent inhibitor
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
P41413
-
-
Homo sapiens
Q92824
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
carotid plaque
Homo sapiens
-
myofibroblast
-
Homo sapiens
-
vascular smooth muscle cell
-
Rattus norvegicus
-
Application (protein specific)
Application
Commentary
Organism
medicine
carotid atherosclerotic lesions obtained by endaterectomy contain PC5, PC5 colocalizes with av integrin
Homo sapiens
medicine
av endoproteolytic activation is necessary for integrin-mediated adhesion and migration as well as signaling and requires PC5 in vascular smooth muscle cells
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
decanoyl-Arg-Val-Lys-Arg-chloromethylketone
0.05 mM inhibits PC activity and consequently av cleavage
Rattus norvegicus
additional information
av cleavage not inhibited by the MMP-inhibitor GM6001 and PC5-specific antisense oligonucleotide-3
Rattus norvegicus
PC5-specific antisense oligonucleotide-1
inhibits PC activity and consequently av cleavage
Rattus norvegicus
PC5-specific antisense oligonucleotide-2
inhibits PC activity and consequently av cleavage, most potent inhibitor
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
carotid plaque
Homo sapiens
-
myofibroblast
-
Homo sapiens
-
vascular smooth muscle cell
-
Rattus norvegicus
-
Other publictions for EC 3.4.21.B26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731128
Heng
Development of a high-throughp ...
Homo sapiens
Anal. Biochem.
475
14-21
2015
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731111
Lee
Proprotein convertase 5/6A is ...
Homo sapiens
Am. J. Respir. Cell Mol. Biol.
52
749-761
2014
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732515
Paule
Proprotein convertase 5/6 clea ...
Homo sapiens
Mol. Hum. Reprod.
21
262-270
2014
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2
1
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731899
Paule
Cleavage of endometrial alpha- ...
Homo sapiens
Hum. Reprod.
27
2766-2774
2012
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4
4
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717191
Kim
Galectin-3 binding protein pro ...
Homo sapiens
Biochem. Biophys. Res. Commun.
404
96-102
2011
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718009
Fritzsche
Time for endothelial cell prop ...
Mus musculus
J. Mol. Med.
89
1061-1063
2011
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1
1
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698386
Fuller
Subtilisin-like proprotein con ...
Homo sapiens
Invest. Ophthalmol. Vis. Sci.
50
5759-5768
2009
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709911
Sun
The proprotein convertase PC5/ ...
Mus musculus
Mol. Cancer
8
73
2009
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680190
Seidah
The activation and physiologic ...
Mus musculus
Int. J. Biochem. Cell Biol.
40
1111-1125
2008
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680909
Mayer
The regulated cell surface zym ...
Mus musculus
J. Biol. Chem.
283
2373-2384
2008
1
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691487
Padua
Regulation of DNA synthesis an ...
Mus musculus
BMC Cell Biol.
9
5
2008
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693094
Remacle
Substrate cleavage analysis of ...
Homo sapiens
J. Biol. Chem.
283
20897-20906
2008
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694893
Essalmani
In vivo functions of the propr ...
Mus musculus
Proc. Natl. Acad. Sci. USA
105
5750-5755
2008
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695251
Xiao
Cell-surface processing of ext ...
Mus musculus
Virology
372
384-397
2008
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679779
Dikeakos
PC1/3, PC2 and PC5/6A are targ ...
Mus musculus
FEBS J.
274
4094-4102
2007
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682018
Fugere
Short polybasic peptide sequen ...
Homo sapiens
Mol. Pharmacol.
71
323-332
2007
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1
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8
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675967
Essalmani
Deletion of the gene encoding ...
Homo sapiens, Mus musculus
Mol. Cell. Biol.
26
354-361
2006
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45
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45
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678042
Stein
RNAi-mediated silencing of pro ...
Mus musculus
Biochem. J.
400
209-215
2006
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680696
Benjannet
The proprotein convertase (PC) ...
Homo sapiens
J. Biol. Chem.
281
30561-30572
2006
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682249
Reynolds Nicole
Inhibition of PC5 expression d ...
Mus musculus
Peptides
27
901-904
2006
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673346
Tang
Decidual differentiation of st ...
Mus musculus
Endocrinology
146
5313-5320
2005
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675051
Okada
Requirement for proprotein con ...
Homo sapiens
J. Clin. Endocrinol. Metab.
90
1028-1034
2005
1
1
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3
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675490
Stawowy Philip
Proprotein convertases furin a ...
Homo sapiens, Rattus norvegicus
J. Mol. Med.
83
865-875
2005
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2
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2
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1
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2
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7
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7
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673108
Stawowy
Endoproteolytic activation of ...
Homo sapiens, Rattus norvegicus
Circulation
109
770-776
2004
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2
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4
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2
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3
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673910
Seidah
-
Proprotein convertase 5 ...
Homo sapiens, Mus musculus, Rattus norvegicus
Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , Eds. ) Academic Press
2
1874-1877
2004
-
2
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1
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1
1
4
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3
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1
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18
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2
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2
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1
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1
1
4
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18
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2
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6
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6
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1
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1
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3
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1
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1
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1
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1
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5
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