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Literature summary for 3.4.21.B25 extracted from

  • Yuasa, K.; Futamatsu, G.; Kawano, T.; Muroshita, M.; Kageyama, Y.; Taichi, H.; Ishikawa, H.; Nagahama, M.; Matsuda, Y.; Tsuji, A.
    Subtilisin-like proprotein convertase paired basic amino acid-cleaving enzyme 4 is required for chondrogenic differentiation in ATDC5 cells (2012), FEBS J., 279, 3997-4009.
    View publication on PubMed
Search for more literature for 3.4.21.B25

Inhibitors

Inhibitors Comment Organism Structure
alpha1-antitrypsin
-
 Mus musculus
decanoyl-Arg-Val-Lys-Arg-chloromethylketone
-
 Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
proform bone morphogenetic protein 6 + H2O Mus musculus BMP6, the substrate is exclusively found in hypertrophic chondrocytes mature bone morphogenetic protein 6 + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
ATDC-5 cell an embryonal carcinoma-derived clonal cell line Mus musculus
-
chondrocyte hypertrophic chondrocyte Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
proform bone morphogenetic protein 6 + H2O BMP6, the substrate is exclusively found in hypertrophic chondrocytes Mus musculus mature bone morphogenetic protein 6 + ?
-
 ?
proform bone morphogenetic protein 6 + H2O BMP6, recombinantly expressed FLAG-tagged human substrate protein. The enzyme recognizes the sequence RTTR(371-374) of human BMP6 and cleaves proBMP6 at its C-terminus Mus musculus mature bone morphogenetic protein 6 + ?
-
 ?

Synonyms

Synonyms Comment Organism
PACE4
-
 Mus musculus
paired basic amino acid-cleaving enzyme 4
-
 Mus musculus
SPC4
-
 Mus musculus
subtilisin-like proprotein convertase 4
-
 Mus musculus

Expression

Organism Comment Expression
Mus musculus enzyme PACE4 mRNA levels increase markedly during chondrogenic differentiation of ATDC-5 cells, overview up

General Information

General Information Comment Organism
evolution the enzyme belongs to the subtilisin-like proprotein convertase (SPC) family Mus musculus
malfunction enzyme PACE4 knockdown causes blockage of chondrogenic differentiation through downregulation of proBMP6 activation Mus musculus
physiological function the enzyme is required for chondrogenic differentiation, e.g. for differentiation of ATDC-5 cells into type II collagen-expressing chondrocytes, type X collagen-expressing hypertrophic chondrocytes, and mineralizing chondrocytes Mus musculus