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Literature summary for 3.4.21.B25 extracted from

  • Mains, R.E.; Berard, C.A.; Denault, J.B.; Zhou, A.; Johnson, R.C.; Leduc, R.
    PACE4: a subtilisin-like endoprotease with unique properties (1997), Biochem. J., 321, 587-593.
No PubMed abstract available
Search for more literature for 3.4.21.B25

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and deletion of the Cys-rich region Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
decanoyl-Arg-Val-Lys-Arg-chloromethylketone 100% inhibition at 0.01 mM Rattus norvegicus
EDTA 78% inhibition at 10 mM Rattus norvegicus
EGTA 71% inhibition at 10 mM Rattus norvegicus
HgCl2 99% inhibition at 1 mM Rattus norvegicus
leupeptin 94% inhibition at 1 mM Rattus norvegicus
ZnCl2 100% inhibition at 2 mM Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
 SDS-PAGE, mature protein Rattus norvegicus
106000
-
 SDS-PAGE, precursor protein Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification cleavage activates the enzyme Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
pituitary gland
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tert-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus tert-butyloxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
PACE4
-
 Rattus norvegicus