Cloned (Comment) | Organism |
---|---|
72 KDa and 43 KDa protein | Treponema denticola |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-mercuribenzenesulfonic acid | 98% inhibition at 0.01 mM | Treponema denticola | |
diisopropyl fluorophosphates | 97% inhibition at 1 mM | Treponema denticola | |
EDTA | 41% inhibition at 5 mM | Treponema denticola | |
Soybean trypsin inhibitor | 49% inhibition at 0.01 mM | Treponema denticola | |
ZnCl2 | 46% inhibition at 1 mM | Treponema denticola |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
three bands at 72000 Da, 43000 Da and 38000 Da after boiling, SDS-PAGE | Treponema denticola |
43000 | - |
three bands at 72000 Da, 43000 Da and 38000 Da after boiling, SDS-PAGE | Treponema denticola |
72000 | - |
three bands at 72000 Da, 43000 Da and 38000 Da after boiling, SDS-PAGE | Treponema denticola |
98000 | - |
two bands at 100000 Da and 98000 Da before boiling, SDS-PAGE | Treponema denticola |
100000 | - |
two bands at 100000 Da and 98000 Da before boiling, SDS-PAGE | Treponema denticola |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Treponema denticola | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
61.7 | - |
- |
Treponema denticola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha1-antitrypsin + H2O | - |
Treponema denticola | ? | - |
? | |
Fibronectin + H2O | - |
Treponema denticola | ? | - |
? | |
Gelatin + H2O | - |
Treponema denticola | ? | - |
? | |
immunogobulin G heavy chain + H2O | weak substrate | Treponema denticola | ? | - |
? | |
additional information | specificity for peptide bonds containing phenylalanine and proline at the P1 and P2 positions | Treponema denticola | ? | - |
? | |
N-succinyl-AAPL-4-nitroanilide + H2O | synthetic substrate | Treponema denticola | ? | - |
? | |
N-succinyl-AAPN-4-nitroanilide + H2O | synthetic substrate | Treponema denticola | ? | - |
? | |
Type IV collagen + H2O | weak substrate | Treponema denticola | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
polymer | x * 72000 + x * 38000 + x * 43000, 72000 Da active protease associates with smaller proteins, SDS-PAGE | Treponema denticola |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Treponema denticola |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Treponema denticola | 72000 Da and 38000 Da protein, calculated from nucleic acid sequence | - |
5.42 |