Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S195A | inactive | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P51124 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
topoisomerase II alpha + H2O | - |
Homo sapiens | ? | cleavage of topoisomerase II alpha by granzyme M at Leu1280 separates topoisomerase II alpha functional domains from the nuclear localization signals and leads to nuclear exit of topoisomerase II alpha catalytic activity | ? |
General Information | Comment | Organism |
---|---|---|
physiological function | granzyme M induced cell death is largely caspase-dependent with various hallmarks of classical apoptosis, coinciding with caspase-independent G2/M cell cycle arrest. The nuclear enzyme topoisomerase II alpha is a physiological substrate of granzyme M. Cleavage of topoisomerase II alpha by granzyme M at Leu1280 separates topoisomerase II alpha functional domains from the nuclear localization signals, leading to nuclear exit of topoisomerase II alpha catalytic activity, thereby rendering it nonfunctional. Similar to the apoptotic phenotype of granzyme M, topoisomerase II alpha depletion in tumor cells leads to cell cycle arrest in G2/M, mitochondrial perturbations, caspase activation, and apoptosis | Homo sapiens |