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Literature summary for 3.4.21.B2 extracted from
- Granzyme M is a regulatory protease that inactivates proteinase inhibitor 9, an endogenous inhibitor of granzyme B (2004), J. Biol. Chem., 279, 54275-54282.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| subcloned from I.M.A.G.E. clone 112558 into vector pPiCza A for expression in Pichia pastoris | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha1-Aantichymotrypsin | - |
Homo sapiens | |
| Alpha1-proteinase inhibitor | - |
Homo sapiens | |
| proteinase inhibitor 9 | - |
Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.37 | - |
acetyl-KVPL-7-amido-4-methylcoumarin | - |
Homo sapiens |  |
| 0.4 | - |
acetyl-KYAL-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 0.72 | - |
acetyl-KVAM-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 0.8 | - |
acetyl-KEPL-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 1 | - |
acetyl-KYPL-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 1.3 | - |
acetyl-VPL-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 1.3 | - |
acetyl-KVPM-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 1.5 | - |
acetyl-RYPL-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 1.8 | - |
acetyl-MEPL-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 2 | - |
acetyl-KYPM-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 2.4 | - |
acetyl-KEPM-7-amido-4-methylcoumarin | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | 83000 | SDS-PAGE, differentially glycosylated species in Pichia pastoris | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by cation exchange chromatography, more than 98% purified | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-GRLL-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | acetyl-GRLL + 7-amino-4-methylcoumarin | - |
? | |
| acetyl-KEPL-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | acetyl-KEPL + 7-amino-4-methylcoumarin | - |
? | |
| acetyl-KEPM-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | acetyl-KEPM + 7-amino-4-methylcoumarin | - |
? | |
| acetyl-KVAM-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | acetyl-KVAM + 7-amino-4-methylcoumarin | - |
? | |
| acetyl-KVPL-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | acetyl-KVPL + 7-amino-4-methylcoumarin | - |
? | |
| acetyl-KVPM-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | acetyl-KVPM + 7-amino-4-methylcoumarin | - |
? | |
| acetyl-KYAL-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | acetyl-KYAL + 7-amino-4-methylcoumarin | - |
? | |
| acetyl-KYPL-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | acetyl-KYPL + 7-amino-4-methylcoumarin | - |
? | |
| acetyl-KYPM-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | acetyl-KYPM + 7-amino-4-methylcoumarin | - |
? | |
| acetyl-MEPL-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | acetyl-MEPL + 7-amino-4-methylcoumarin | - |
? | |
| acetyl-RYPL-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | acetyl-RYPL + 7-amino-4-methylcoumarin | - |
? | |
| acetyl-VPL-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | acetyl-VPL + 7-amino-4-methylcoumarin | - |
? | |
| additional information | preference of proline over alanine at P2 and leucine over norleucine over methionine at P1, broad specificity at P3 and P4 | Homo sapiens | ? | - |
? | |
| proteinase inhibitor 9 + H2O | effective hydrolysis and inactivation, bypassing proteinase inhibitor 9 inhibition of granzyme B | Homo sapiens | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0044 | - |
acetyl-VPL-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 0.06 | - |
acetyl-KYAL-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 0.077 | - |
acetyl-KVAM-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 0.115 | - |
acetyl-KYPM-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 0.142 | - |
acetyl-MEPL-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 0.22 | - |
acetyl-RYPL-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 0.42 | - |
acetyl-KEPM-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 0.7 | - |
acetyl-KVPL-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 0.74 | - |
acetyl-KVPM-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 0.77 | - |
acetyl-KYPL-7-amido-4-methylcoumarin | - |
Homo sapiens | |
| 0.79 | - |
acetyl-KEPL-7-amido-4-methylcoumarin | - |
Homo sapiens | |
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