Cloned (Comment) | Organism |
---|---|
into transfer vector pBacPAK8, expressed in Sf21 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | a series of peptide phosphonates and chloromethyl ketones tested, neither inhibitor type proved to be especially potent toward the enzyme, including the general serine protease inhibitor, 3,4-dichloroisocoumarin | Homo sapiens | |
additional information | a series of peptide phosphonates and chloromethyl ketones tested, neither inhibitor type proved to be especially potent toward the enzyme, including the general serine protease inhibitor, 3,4-dichloroisocoumarin | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0263 | - |
N2-[(benzyloxy)carbonyl]-N6-(tert-butoxycarbonyl)-L-lysyl-L-prolyl-L-methionyl thiobenzyl ester | - |
Homo sapiens | |
0.0334 | - |
N2-Cbz-L-Asp(O-tBut)-L-Pro-L-Met thiobenzyl ester | - |
Homo sapiens | |
0.036 | - |
N-benzyloxycarbonyl-phenylalanyl-prolyl-methionine-thiobenzylester | - |
Homo sapiens | |
0.0366 | - |
N2-CBZ-O3-tBut-L-Ser-L-Pro-L-Met-SBzl | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.0423 | - |
N2-CBZ-O3-tBut-L-Ser-L-Pro-L-Met-SBzl | - |
Homo sapiens | |
0.079 | - |
N-benzyloxycarbonyl-alanyl-alanyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.083 | - |
N2-[(benzyloxy)carbonyl]-N6-(tert-butoxycarbonyl)-L-lysyl-L-prolyl-L-methionyl thiobenzyl ester | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.0915 | - |
N-benzyloxycarbonyl-prolyl-prolyl-methionine-thiobenzylester | - |
Homo sapiens | |
0.129 | - |
N2-Cbz-L-Asp(O-tBut)-L-Pro-L-Met thiobenzyl ester | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.181 | - |
N-benzyloxycarbonyl-phenylalanyl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.184 | - |
N-benzyloxycarbonyl-alanyl-alanyl-methionine-thiobenzylester | murine granzyme M without bovine serum albumin | Homo sapiens | |
0.188 | - |
N-benzyloxycarbonyl-alanyl-alanyl-prolyl-methionine-thiobenzylester | - |
Homo sapiens | |
0.21 | - |
N-benzyloxycarbonyl-alanyl-seryl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.217 | - |
N-benzyloxycarbonyl-prolyl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.22 | - |
N-benzyloxycarbonyl-alanyl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.236 | - |
N-benzyloxycarbonyl-alanyl-alanyl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.267 | - |
N-benzyloxycarbonyl-alanyl-aspartyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.275 | - |
N-benzyloxycarbonyl-alanyl-prolyl-methionine-thiobenzylester | - |
Homo sapiens | |
0.361 | - |
N-benzyloxycarbonyl-lysyl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.418 | - |
N-benzyloxycarbonyl-aspartyl-prolyl-methionine-thiobenzylester | - |
Homo sapiens | |
0.441 | - |
N-benzyloxycarbonyl-aspartyl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.635 | - |
N-benzyloxycarbonyl-alanyl-alanyl-methionine-thiobenzylester | - |
Homo sapiens | |
0.694 | - |
N-benzyloxycarbonyl-alanyl-prolyl-methionine-thiobenzylester | murine granzyme M without bovine serum albumin | Homo sapiens | |
0.695 | - |
N-benzyloxycarbonyl-alanyl-aspartyl-methionine-thiobenzylester | - |
Homo sapiens | |
0.793 | - |
N-benzyloxycarbonyl-alanyl-lysyl-methionine-thiobenzylester | murine granzyme M without bovine serum albumin | Homo sapiens | |
0.894 | - |
N-benzyloxycarbonyl-lysyl-prolyl-methionine-thiobenzylester | - |
Homo sapiens | |
0.92 | - |
N-benzyloxycarbonyl-seryl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
1.02 | - |
N-benzyloxycarbonyl-alanyl-alanyl-prolyl-methionine-thiobenzylester | murine granzyme M without bovine serum albumin | Homo sapiens | |
1.3 | - |
N-benzyloxycarbonyl-alanyl-seryl-methionine-thiobenzylester | - |
Homo sapiens | |
2.66 | - |
N-benzyloxycarbonyl-alanyl-seryl-methionine-thiobenzylester | murine granzyme M without bovine serum albumin | Homo sapiens | |
3.89 | - |
N-benzyloxycarbonyl-alanyl-lysyl-methionine-thiobenzylester | - |
Homo sapiens | |
6.65 | - |
N-benzyloxycarbonyl-seryl-prolyl-methionine-thiobenzylester | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | 35000 | SDS-PAGE, glycosylated forms | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P51124 | - |
- |
Rattus norvegicus | Q03238 | - |
- |
Purification (Comment) | Organism |
---|---|
by Ni-affinity chromatography | Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, Hepes buffer, pH 7.5, several months | Homo sapiens |
4°C, Hepes buffer, pH 7.5, several months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme has a strong preference for Pro at the P2 position and Ala, Ser, or Asp at the P3 position | Homo sapiens | ? | - |
? | |
N-benzyloxycarbonyl-alanyl-alanyl-methionine-thiobenzylester + H2O | - |
Homo sapiens | N-benzyloxycarbonyl-alanyl-alanyl-methionine + phenylmethanethiol | - |
? | |
N-benzyloxycarbonyl-alanyl-alanyl-prolyl-methionine-thiobenzylester + H2O | - |
Homo sapiens | N-benzyloxycarbonyl-alanyl-alanyl-prolyl-methionine + phenylmethanethiol | - |
? | |
N-benzyloxycarbonyl-alanyl-aspartyl-methionine-thiobenzylester + H2O | - |
Homo sapiens | -benzyloxycarbonyl-alanyl-aspartyl-methionine + phenylmethanethiol | - |
? | |
N-benzyloxycarbonyl-alanyl-lysyl-methionine-thiobenzylester + H2O | - |
Homo sapiens | N-benzyloxycarbonyl-alanyl-lysyl-methionine + phenylmethanethiol | - |
? | |
N-benzyloxycarbonyl-alanyl-prolyl-methionine-thiobenzylester + H2O | - |
Homo sapiens | N-benzyloxycarbonyl-alanyl-prolyl-methionine + phenylmethanethiol | - |
? | |
N-benzyloxycarbonyl-alanyl-seryl-methionine-thiobenzylester + H2O | - |
Homo sapiens | N-benzyloxycarbonyl-alanyl-seryl-methionine + phenylmethanethiol | - |
? | |
N-benzyloxycarbonyl-aspartyl-prolyl-methionine-thiobenzylester + H2O | - |
Homo sapiens | N-benzyloxycarbonyl-aspartyl-prolyl-methionine + phenylmethanethiol | - |
? | |
N-benzyloxycarbonyl-L-Phe-L-Pro-L-Met-thiobenzyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
N-benzyloxycarbonyl-lysyl-prolyl-methionine-thiobenzylester + H2O | - |
Homo sapiens | N-benzyloxycarbonyl-lysyl-prolyl-methionine + phenylmethanethiol | - |
? | |
N-benzyloxycarbonyl-prolyl-prolyl-methionine-thiobenzylester + H2O | - |
Homo sapiens | N-benzyloxycarbonyl-prolyl-prolyl-methionine + phenylmethanethiol | - |
? | |
N-benzyloxycarbonyl-seryl-prolyl-methionine-thiobenzylester + H2O | - |
Homo sapiens | N-benzyloxycarbonyl-seryl-prolyl-methionine + phenylmethanethiol | - |
? | |
N2-Cbz-L-Asp(O-tBut)-L-Pro-L-Met thiobenzyl ester + H2O | - |
Homo sapiens | N2-Cbz-L-Asp(O-tBut)-L-Pro-L-Met + phenylmethanethiol | - |
? | |
N2-CBZ-O3-tBut-L-Ser-L-Pro-L-Met-SBzl + H2O | - |
Homo sapiens | N2-CBZ-O3-tBut-L-Ser-L-Pro-L-Met + phenylmethanethiol | - |
? | |
N2-[(benzyloxy)carbonyl]-N6-(tert-butoxycarbonyl)-L-lysyl-L-prolyl-L-methionyl thiobenzyl ester + H2O | - |
Homo sapiens | N2-[(benzyloxy)carbonyl]-N6-(tert-butoxycarbonyl)-L-lysyl-L-prolyl-L-methionine + phenylmethanethiol | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00785 | - |
N-benzyloxycarbonyl-prolyl-prolyl-methionine-thiobenzylester | - |
Homo sapiens | |
0.03 | - |
N2-[(benzyloxy)carbonyl]-N6-(tert-butoxycarbonyl)-L-lysyl-L-prolyl-L-methionyl thiobenzyl ester | - |
Homo sapiens | |
0.031 | 0.51 | N-benzyloxycarbonyl-lysyl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.042 | - |
N2-Cbz-L-Asp(O-tBut)-L-Pro-L-Met thiobenzyl ester | - |
Homo sapiens | |
0.0784 | - |
N-benzyloxycarbonyl-phenylalanyl-prolyl-methionine-thiobenzylester | - |
Homo sapiens | |
0.0903 | - |
N-benzyloxycarbonyl-alanyl-aspartyl-methionine-thiobenzylester | - |
Homo sapiens | |
0.0917 | - |
N2-CBZ-O3-tBut-L-Ser-L-Pro-L-Met-SBzl | - |
Homo sapiens | |
0.161 | - |
N2-CBZ-O3-tBut-L-Ser-L-Pro-L-Met-SBzl | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.26 | - |
N-benzyloxycarbonyl-seryl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.335 | - |
N2-[(benzyloxy)carbonyl]-N6-(tert-butoxycarbonyl)-L-lysyl-L-prolyl-L-methionyl thiobenzyl ester | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.438 | - |
N-benzyloxycarbonyl-alanyl-aspartyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.634 | - |
N-benzyloxycarbonyl-phenylalanyl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
0.973 | - |
N-benzyloxycarbonyl-prolyl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
1.68 | - |
N-benzyloxycarbonyl-alanyl-seryl-methionine-thiobenzylester | - |
Homo sapiens | |
2.13 | - |
N2-Cbz-L-Asp(O-tBut)-L-Pro-L-Met thiobenzyl ester | murine granzyme M with bovine serum albumin | Homo sapiens | |
2.46 | - |
N-benzyloxycarbonyl-alanyl-alanyl-methionine-thiobenzylester | murine granzyme M without bovine serum albumin | Homo sapiens | |
3.56 | - |
N-benzyloxycarbonyl-alanyl-seryl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
4.35 | - |
N-benzyloxycarbonyl-alanyl-alanyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
6.07 | - |
N-benzyloxycarbonyl-alanyl-lysyl-methionine-thiobenzylester | murine granzyme M without bovine serum albumin | Homo sapiens | |
6.08 | - |
N-benzyloxycarbonyl-phenylalanyl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
6.08 | - |
N-benzyloxycarbonyl-prolyl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
8.01 | - |
N-benzyloxycarbonyl-lysyl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
9.68 | - |
N-benzyloxycarbonyl-alanyl-lysyl-methionine-thiobenzylester | - |
Homo sapiens | |
10.6 | - |
N-benzyloxycarbonyl-alanyl-prolyl-methionine-thiobenzylester | - |
Homo sapiens | |
10.8 | - |
N-benzyloxycarbonyl-alanyl-alanyl-methionine-thiobenzylester | - |
Homo sapiens | |
12.3 | - |
N-benzyloxycarbonyl-alanyl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
12.8 | - |
N-benzyloxycarbonyl-alanyl-seryl-methionine-thiobenzylester | murine granzyme M without bovine serum albumin | Homo sapiens | |
13.1 | - |
N-benzyloxycarbonyl-alanyl-alanyl-prolyl-methionine-thiobenzylester | - |
Homo sapiens | |
13.1 | - |
N-benzyloxycarbonyl-lysyl-prolyl-methionine-thiobenzylester | - |
Homo sapiens | |
16.1 | - |
N-benzyloxycarbonyl-alanyl-alanyl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
17.3 | - |
N-benzyloxycarbonyl-aspartyl-prolyl-methionine-thiobenzylester | - |
Homo sapiens | |
18.1 | - |
N-benzyloxycarbonyl-alanyl-prolyl-methionine-thiobenzylester | murine granzyme M without bovine serum albumin | Homo sapiens | |
29 | - |
N-benzyloxycarbonyl-aspartyl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
30 | - |
N-benzyloxycarbonyl-alanyl-alanyl-prolyl-methionine-thiobenzylester | murine granzyme M without bovine serum albumin | Homo sapiens | |
52.1 | - |
N-benzyloxycarbonyl-seryl-prolyl-methionine-thiobenzylester | murine granzyme M with bovine serum albumin | Homo sapiens | |
255 | - |
N-benzyloxycarbonyl-seryl-prolyl-methionine-thiobenzylester | - |
Homo sapiens |