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Literature summary for 3.4.21.B12 extracted from

  • Simmer, J.P.; Fukae, M.; Tanabe, T.; Yamakoshi, Y.; Uchida, T.; Xue, J.; Margolis, H.C.; Shimizu, M.; DeHart, B.C.; Hu, J.C.C.; Bartlett, J.D.
    Purification, characterization, and cloning of enamel matrix serine proteinase 1 (1998), J. Dent. Res., 77, 377-386.
    View publication on PubMed
Search for more literature for 3.4.21.B12

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Sus scrofa 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polypeptide + H2O Sus scrofa
-
 peptides
-
 ?
polypeptide + H2O Sus scrofa EMSP1
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa Q9XSN6 EMSP1
-
Sus scrofa EMSP1 Q9XSN6 EMSP1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 3 potential N-linked glycosylation sites: N134, N169, and N214 of the nucleotide sequence Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
proteolysis of polypeptides active site residues: H71, D116, and S207 Sus scrofa
a

Source Tissue

Source Tissue Comment Organism Textmining
dental pulp
-
 Sus scrofa
-
enamel transition-stage developing matrix Sus scrofa
-
epithelium of enamel organ Sus scrofa
-
additional information enzyme expression is restricted to developimg teeth Sus scrofa
-
tooth bud unerupted, incisor Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amelogenin + H2O 20 kDa substrate Sus scrofa amelogenin fragments
-
 ?
amelogenin + H2O 20 kDa substrate Sus scrofa EMSP1 amelogenin fragments
-
 ?
Gelatin + H2O zymograph assay Sus scrofa ?
-
 ?
Gelatin + H2O zymograph assay Sus scrofa EMSP1 ?
-
 ?
polypeptide + H2O
-
 Sus scrofa peptides
-
 ?
polypeptide + H2O
-
 Sus scrofa EMSP1 peptides
-
 ?

Subunits

Subunits Comment Organism
? x * 24100-34000, SDS-PAGE and amino acid sequence determination Sus scrofa

Synonyms

Synonyms Comment Organism
EMSP1
-
 Sus scrofa
serine protease 1
-
 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at, substrate 20 kDa amelogenin Sus scrofa
7.5
-
 assay at, substrate gelatin Sus scrofa

pI Value

Organism Comment pI Value Maximum pI Value
Sus scrofa
-
-
 6