Literature summary for 3.4.21.B10 extracted from

Luo, L.; Soosaipillai, A.; Grass, L.; Diamandis, E.P.
Characterization of human kallikreins 6 and 10 in ascites fluid from ovarian cancer patients (2006), Tumour Biol., 27, 227-234.

Search for more literature for 3.4.21.B10

Application

Application	Comment	Organism
medicine	active hK6 is present in ovarian cancer ascites fluid, provides a rationale for the development of novel therapeutic strategies	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	activity of hK6 can be suppressed by ca. 60% by monoclonal antibody E24	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.23	-	Pro-Phe-Arg-7-amido-4-methylcoumarin	purified hK6	Homo sapiens
0.3	-	Phe-Ser-Arg-7-amido-4-methylcoumarin	purified hK6	Homo sapiens
0.36	-	Val-Pro-Arg-7-amido-4-methylcoumarin	purified hK6	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	purified hK6, Western blot analysis	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
hK6 purified on immunoaffinity columns, followed by reverse-phase high performance liquid chromatography, to homogeneity	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	ovarian cancer ascites fluid from ovarian cancer patients during therapeutic drainage. hK6 and hK10 concentrations are positively correlated in ovarian cancer ascites fluid, majority of hK6 present in the free (uncomplexed) form	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	hK6 shows weak trypsin-like enzymatic activity, does not cleave Val-Leu-Lys-7-amido-4-methylcoumarin, Glu-Lys-Lys-7-amido-4-methylcoumarin and Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin	Homo sapiens	?	-	?
Phe-Ser-Arg-7-amido-4-methylcoumarin	purified hK6	Homo sapiens	Phe-Ser-Arg + 7-amino-4-methylcoumarin	-	?
Pro-Phe-Arg-7-amido-4-methylcoumarin	purified hK6	Homo sapiens	Pro-Phe-Arg + 7-amino-4-methylcoumarin	-	?
Val-Pro-Arg-7-amido-4-methylcoumarin	purified hK6	Homo sapiens	Val-Pro-Arg + 7-amino-4-methylcoumarin	-	?

Subunits

Subunits	Comment	Organism
?	x * 30000, SDS-PAGE, purified hK6	Homo sapiens

Synonyms

Synonyms	Comment	Organism
hK6	-	Homo sapiens
kallikrein 6	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0003	-	Pro-Phe-Arg-7-amido-4-methylcoumarin	purified hK6	Homo sapiens
0.00083	-	Phe-Ser-Arg-7-amido-4-methylcoumarin	purified hK6	Homo sapiens
0.0013	-	Val-Pro-Arg-7-amido-4-methylcoumarin	purified hK6	Homo sapiens

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Release 2023.1 (January 2023)