Activating Compound | Comment | Organism | Structure |
---|---|---|---|
chondroitin sulfate | activates hK6 in a concentration-dependent manner | Homo sapiens | |
dermatan sulfate | activates hK6 in a concentration-dependent manner | Homo sapiens | |
heparan sulfate | activates hK6 in a concentration-dependent manner | Homo sapiens | |
heparin | activates hK6 in a concentration-dependent manner | Homo sapiens | |
Sodium citrate | reveals greatest activity at pH 7.5 | Homo sapiens | |
Sodium sulfate | reveals greatest activity at pH 7.5 | Homo sapiens |
Application | Comment | Organism |
---|---|---|
additional information | hK6 is involved in normal myelin turnover/demyelination processes, but it is unlikely to self-activate. Possibly it modulates ionotropic glutamate receptors and activates PAR 2 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed from a baculovirus/insect cell line system | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.005 mM completely inhibits hK6 at pH 7.5 or 9.0 | Homo sapiens | |
K+ | inhibits hK6 at pH 7.5 or 9.0 | Homo sapiens | |
Mg2+ | 0.005 mM completely inhibits hK6 at pH 7.5 or 9.0 | Homo sapiens | |
NH4+ | inhibits hK6 at pH 7.5 or 9.0 | Homo sapiens | |
Sodium citrate | inhibits the enzyme at pH 9.0 | Homo sapiens | |
Sodium sulfate | inhibits the enzyme at pH 9.0 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
o-aminobenzoic acid-AFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
0.0003 | - |
o-aminobenzoic acid-AFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
0.001 | - |
o-aminobenzoic acid-AFRVGQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
0.0011 | - |
o-aminobenzoic acid-AFRLAQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
0.0014 | - |
o-aminobenzoic acid-EEHAFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
0.0016 | - |
o-aminobenzoic acid-EHSAFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
0.0028 | - |
o-aminobenzoic acid-KLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
0.004 | - |
o-aminobenzoic acid-KLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 9, without salt | Homo sapiens | |
0.009 | - |
o-aminobenzoic acid-AFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 9, without salt | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha1-Antichymotrypsin + H2O | hydrolysis of the FRET peptide derived from alpha1-antichymotrypsin | Homo sapiens | ? | - |
? | |
additional information | hydrolysis of the FRET peptide series o-aminobenzoic acid-XLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine, o-aminobenzoic acid-KXRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine, o-aminobenzoic acid-KLRXSKQ-N-[2,4-dinitrophenyl]ethylenediamine, o-aminobenzoic acid-KLRSXKQ-N-[2,4-dinitrophenyl]ethylenediamine and FRET peptide derived from hK6 maturation/inactivation cleavage site or PAR. o-aminobenzoic acid-AFKFAQ-N-[2,4-dinitrophenyl]ethylenediamine, o-aminobenzoic acid-VLFEKKVYLQ-N-[2,4-dinitrophenyl]ethylenediamine and o-aminobenzoic acid-GTEASVVAPQ-N-[2,4-dinitrophenyl]ethylenediamine are not hydrolyzed | Homo sapiens | ? | - |
? | |
myelin basic protein + H2O | hydrolysis of the FRET peptide derived from human myelin basic protein | Homo sapiens | ? | - |
? | |
o-aminobenzoic acid-AFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O | - |
Homo sapiens | ? | - |
? | |
o-aminobenzoic acid-AFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O | best synthetic substrate | Homo sapiens | ? | - |
? | |
o-aminobenzoic acid-AFRLAQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O | - |
Homo sapiens | ? | - |
? | |
o-aminobenzoic acid-AFRVGQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O | - |
Homo sapiens | ? | - |
? | |
o-aminobenzoic acid-EEHAFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O | - |
Homo sapiens | ? | - |
? | |
o-aminobenzoic acid-EELAFKFAQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O | - |
Homo sapiens | ? | - |
? | |
o-aminobenzoic acid-EHSAFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O | - |
Homo sapiens | ? | - |
? | |
o-aminobenzoic acid-KLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O | - |
Homo sapiens | ? | - |
? | |
plasminogen + H2O | hydrolysis of the FRET peptide derived from plasminogen | Homo sapiens | ? | - |
? | |
precursor of the Abeta amyloid peptid + H2O | hydrolysis of the FRET peptide derived from human precursor of the Abeta amyloid peptide | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hK6 | - |
Homo sapiens |
kallikrein 6 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
o-aminobenzoic acid-AFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 9, without salt | Homo sapiens | |
2.3 | - |
o-aminobenzoic acid-KLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 9, without salt | Homo sapiens | |
2.7 | - |
o-aminobenzoic acid-AFRVGQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
7.1 | - |
o-aminobenzoic acid-EEHAFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
7.1 | - |
o-aminobenzoic acid-AFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
9.1 | - |
o-aminobenzoic acid-EHSAFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
9.2 | - |
o-aminobenzoic acid-AFRLAQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
11.6 | - |
o-aminobenzoic acid-AFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
14.2 | - |
o-aminobenzoic acid-KLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Homo sapiens |