Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2'-deoxy-5-azacytidine | can reactivate KLK6 in non-expressing breast cancer cells. Induces KLK6 expression in cell lines MDA-MB-435P, BT-474, MCF-7, and ZR-75-1 and significantly increases synthesis of hK6 protein. Results in a 2fold increase in KLK6 mRNA and protein levels in HMECs. Combined 5-aza-29-deoxycytidine/trichostatin A treatment results in synergistic activation of KLK6 in MDA-MB-231 cells | Homo sapiens | |
EB1089 | vitamin D3 analog EB1089 can restore KLK6 expression in T47D cells | Homo sapiens | |
trichostatin A | moderately induces KLK6 in MDA-MB-231 cells. Combined 5-aza-29-deoxycytidine/trichostatin A treatment results in synergistic activation of KLK6 in MDA-MB-231 cells | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | multiple co-operating mechanisms underlie the inactivation of KLK6 expression in breast cancer and provide ways for its pharmacological modulation with potential therapeutic implications | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
KLK6 genomic region from -80 to +244 relative to the P1 transcriptional start site cloned upstream from the CAT reporter gene. PBLCAT6 (-80,+244) construct and two deletion constructs, pBLCAT6 (-80,+179) and pBLCAT6(-80,+12), transfected into MCF-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion constructs pBLCAT6 (-80,+179) and pBLCAT6(-80,+12) do not show transactivation. KLK6 sequence downstream of +179 is important for KLK6 transcription | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q92876 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | barely expresses KLK6 | Homo sapiens | - |
HMEC-1 cell | - |
Homo sapiens | - |
MCF-7 cell | MCF-7 cells with low metastatic potential express KLK6 mRNA and protein levels comparable to normal human mammary epithelial cells | Homo sapiens | - |
MDA-MB-468 cell | - |
Homo sapiens | - |
additional information | 21PT cell isolated from a primary breast tumor, highly overexpresses KLK6. KLK6 expression is absent or very low in metastatic breast cancer cell lines MDA-MB-231, BT-474, MDA-MB-435, MDAMB-435P, ZR-75-1 and T47D | Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
hK6 | expression from KLK6 gene | Homo sapiens |
kallikrein 6 | - |
Homo sapiens |
KLK6 | - |
Homo sapiens |