Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.B10 extracted from

  • Pampalakis, G.; Sotiropoulou, G.
    Multiple mechanisms underlie the aberrant expression of the human kallikrein 6 gene in breast cancer (2006), Biol. Chem., 387, 773-782.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2'-deoxy-5-azacytidine can reactivate KLK6 in non-expressing breast cancer cells. Induces KLK6 expression in cell lines MDA-MB-435P, BT-474, MCF-7, and ZR-75-1 and significantly increases synthesis of hK6 protein. Results in a 2fold increase in KLK6 mRNA and protein levels in HMECs. Combined 5-aza-29-deoxycytidine/trichostatin A treatment results in synergistic activation of KLK6 in MDA-MB-231 cells Homo sapiens
EB1089 vitamin D3 analog EB1089 can restore KLK6 expression in T47D cells Homo sapiens
trichostatin A moderately induces KLK6 in MDA-MB-231 cells. Combined 5-aza-29-deoxycytidine/trichostatin A treatment results in synergistic activation of KLK6 in MDA-MB-231 cells Homo sapiens

Application

Application Comment Organism
medicine multiple co-operating mechanisms underlie the inactivation of KLK6 expression in breast cancer and provide ways for its pharmacological modulation with potential therapeutic implications Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
KLK6 genomic region from -80 to +244 relative to the P1 transcriptional start site cloned upstream from the CAT reporter gene. PBLCAT6 (-80,+244) construct and two deletion constructs, pBLCAT6 (-80,+179) and pBLCAT6(-80,+12), transfected into MCF-7 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information deletion constructs pBLCAT6 (-80,+179) and pBLCAT6(-80,+12) do not show transactivation. KLK6 sequence downstream of +179 is important for KLK6 transcription Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q92876
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell barely expresses KLK6 Homo sapiens
-
HMEC-1 cell
-
Homo sapiens
-
MCF-7 cell MCF-7 cells with low metastatic potential express KLK6 mRNA and protein levels comparable to normal human mammary epithelial cells Homo sapiens
-
MDA-MB-468 cell
-
Homo sapiens
-
additional information 21PT cell isolated from a primary breast tumor, highly overexpresses KLK6. KLK6 expression is absent or very low in metastatic breast cancer cell lines MDA-MB-231, BT-474, MDA-MB-435, MDAMB-435P, ZR-75-1 and T47D Homo sapiens
-

Synonyms

Synonyms Comment Organism
hK6 expression from KLK6 gene Homo sapiens
kallikrein 6
-
Homo sapiens
KLK6
-
Homo sapiens