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Literature summary for 3.4.21.98 extracted from
- Insights to substrate binding and processing by West Nile Virus NS3 protease through combined modeling, protease mutagenesis, and kinetic studies (2006), J. Biol. Chem., 281, 38448-38458.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | optimization of tetrapeptide substrates for enhanced protease affinity and processing efficiency provides important clues for developing inhibitors of West Nile Virus infection | West Nile virus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure of NS2B/NS3 protease reveals that Asn-84 of the NS2B cofactor is within hydrogen bonding distance of the P2 Lys of the bound ligand.Asn-84 is located within a highly conserved region of the cofactor, the Gly residue on the N-terminal side is completely conserved, and the third residue on the C-terminal side is a highly conserved hydrophobic Leu or Ile | West Nile virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A164S | impaired function compared with wild type enzyme, decreases in substrate affinity | West Nile virus |
| A164V | impaired function compared with wild type enzyme, decreases in substrate affinity | West Nile virus |
| I155F | impaired function compared with wild type enzyme, decreases in substrate affinity | West Nile virus |
| I162F | impaired function compared with wild type enzyme, decreases in substrate affinity | West Nile virus |
| M156A | impaired function compared with wild type enzyme, decreases in substrate affinity | West Nile virus |
| T111F | impaired function compared with wild type enzyme, decreases in substrate affinity | West Nile virus |
| T111L | impaired function compared with wild type enzyme, decreases in substrate affinity | West Nile virus |
| V154F | impaired function compared with wild type enzyme, decreases in substrate affinity | West Nile virus |
| V154L | impaired function compared with wild type enzyme, decreases in substrate affinity | West Nile virus |
| V166L | impaired function compared with wild type enzyme, decreases in substrate affinity | West Nile virus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
2-Naph-Cit-Lys-Arg-p-nitroanilide | - |
West Nile virus |  |
| 0.0229 | - |
2-Naph-Orn-Orn-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.0254 | - |
2-Naph-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.0267 | - |
2-Naph-Lys-hArg-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.0368 | - |
2-Naph-Lys-Orn-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.038 | - |
2-Naph-Orn-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.0434 | - |
2-Naph-Lys-Arg-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.048 | - |
2-Naph-hArg-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.0546 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.0625 | - |
Ac-Leu-hArg-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.0658 | - |
2-Naph-3Pya-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.0673 | - |
Ac-Nva-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.0699 | - |
Ac-Cha-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.0755 | - |
Ac-Aoc-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.0797 | - |
Ac-Aib-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.0807 | - |
Ac-Nle-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.0819 | - |
Ac-Chg-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.0887 | - |
Ac-Leu-3Pya-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.096 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant V166L | West Nile virus | |
| 0.0977 | - |
2-Naph-Lys-Lys-Arg-p-nitroanilide | NS3 mutant V154F | West Nile virus | |
| 0.1007 | - |
2-Naph-Cit-Orn-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.1055 | - |
Ac-Leu-Orn-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.1342 | - |
Ac-Tbg-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.1413 | - |
Ac-Leu-aPhe-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.164 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant M156A | West Nile virus | |
| 0.169 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant T111F | West Nile virus | |
| 0.19 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant V154L | West Nile virus | |
| 0.199 | - |
Ac-Leu-Gln-Tyr-Thr-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.207 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant I155F | West Nile virus | |
| 0.2086 | - |
Ac-Leu-Cit-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.214 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant T111L | West Nile virus | |
| 0.221 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant A164S | West Nile virus | |
| 0.232 | - |
Ac-Leu-Gln-Tyr-Thr-Lys-Arg-p-nitroanilide | NS3 mutant V166L | West Nile virus | |
| 0.2541 | - |
Ac-Leu-nPhe-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.2548 | - |
Ac-Leu-4Pya-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.263 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant V154F | West Nile virus | |
| 0.268 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant I162F | West Nile virus | |
| 0.331 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant A164V | West Nile virus | |
| 0.344 | - |
Ac-Leu-Gln-Tyr-Thr-Lys-Arg-p-nitroanilide | NS3 mutant V154L | West Nile virus | |
| 0.376 | - |
Ac-Leu-Gln-Tyr-Thr-Lys-Arg-p-nitroanilide | NS3 mutant T111F | West Nile virus | |
| 0.416 | - |
Ac-Leu-Gln-Tyr-Thr-Lys-Arg-p-nitroanilide | NS3 mutant I162F | West Nile virus | |
| 0.432 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant D129E | West Nile virus | |
| 0.617 | - |
Ac-Leu-Gln-Tyr-Thr-Lys-Arg-p-nitroanilide | NS3 mutant T111L | West Nile virus | |
| 0.635 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant D129A | West Nile virus | |
| 0.879 | - |
Ac-Leu-Gln-Tyr-Thr-Lys-Arg-p-nitroanilide | NS3 mutant M156A | West Nile virus | |
| 2.303 | - |
2-Naph-Lys-AcLys-Arg-p-nitroanilide | - |
West Nile virus | |
| 3.423 | - |
2-Naph-Lys-Cit-Arg-p-nitroanilide | - |
West Nile virus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| West Nile virus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
West Nile virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-Naph-3Pya-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| 2-Naph-Cit-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| 2-Naph-Cit-Orn-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| 2-Naph-hArg-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| 2-Naph-Lys-AcLys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| 2-Naph-Lys-Arg-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| 2-Naph-Lys-Cit-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| 2-Naph-Lys-hArg-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| 2-Naph-Lys-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| 2-Naph-Lys-Orn-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| 2-Naph-Orn-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| 2-Naph-Orn-Orn-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| Ac-Aib-Lys-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| Ac-Aoc-Lys-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| Ac-Cha-Lys-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| Ac-Chg-Lys-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| Ac-Leu-3Pya-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| Ac-Leu-4Pya-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| Ac-Leu-aPhe-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| Ac-Leu-Cit-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| Ac-Leu-Gln-Tyr-Thr-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| Ac-Leu-hArg-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| Ac-Leu-Lys-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| Ac-Leu-nPhe-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| Ac-Leu-Orn-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| Ac-Nle-Lys-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| Ac-Nva-Lys-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| Ac-Tbg-Lys-Lys-Arg-p-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
| additional information | no activity with 2-Naph-Lys-aPhe-Arg-p-nitroanilide, 2-Naph-Lys-nLeu-Arg-p-nitroanilide and 2-Naph-Lys-Phe-Arg-p-nitroanilide | West Nile virus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NS2B/NS3 protease | - |
West Nile virus |
| NS3 protease | - |
West Nile virus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
Ac-Leu-Gln-Tyr-Thr-Lys-Arg-p-nitroanilide | NS3 mutant T111L | West Nile virus | |
| 0.036 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant D129E | West Nile virus | |
| 0.04 | - |
Ac-Leu-Gln-Tyr-Thr-Lys-Arg-p-nitroanilide | NS3 mutant I162F | West Nile virus | |
| 0.0431 | - |
2-Naph-Lys-Lys-Arg-p-nitroanilide | NS3 mutant V154F | West Nile virus | |
| 0.048 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant D129A | West Nile virus | |
| 0.051 | - |
Ac-Leu-Gln-Tyr-Thr-Lys-Arg-p-nitroanilide | NS3 mutant T111F | West Nile virus | |
| 0.057 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant V154F | West Nile virus | |
| 0.06 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant T111L | West Nile virus | |
| 0.11 | - |
2-Naph-hArg-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.164 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant I162F | West Nile virus | |
| 0.181 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant A164V | West Nile virus | |
| 0.198 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant T111F | West Nile virus | |
| 0.245 | - |
Ac-Leu-Gln-Tyr-Thr-Lys-Arg-p-nitroanilide | NS3 mutant V154L | West Nile virus | |
| 0.26 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant A164S | West Nile virus | |
| 0.266 | - |
Ac-Leu-Gln-Tyr-Thr-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.27 | - |
2-Naph-Orn-Orn-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.297 | - |
Ac-Leu-Gln-Tyr-Thr-Lys-Arg-p-nitroanilide | NS3 mutant V166L | West Nile virus | |
| 0.3 | - |
2-Naph-Lys-AcLys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.314 | - |
Ac-Leu-Gln-Tyr-Thr-Lys-Arg-p-nitroanilide | NS3 mutant M156A | West Nile virus | |
| 0.32 | - |
2-Naph-Cit-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.43 | - |
2-Naph-Lys-Cit-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.5 | - |
Ac-Leu-3Pya-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.57 | - |
2-Naph-Cit-Orn-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.59 | - |
2-Naph-Lys-hArg-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.638 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant V166L | West Nile virus | |
| 0.72 | - |
Ac-Leu-nPhe-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.733 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant I155F | West Nile virus | |
| 0.75 | - |
Ac-Leu-aPhe-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.778 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant M156A | West Nile virus | |
| 0.8 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | NS3 mutant V154L | West Nile virus | |
| 0.89 | - |
2-Naph-Lys-Arg-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.9 | - |
Ac-Leu-4Pya-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.96 | - |
2-Naph-3Pya-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 0.98 | - |
2-Naph-Orn-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 1.08 | - |
2-Naph-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 1.44 | - |
Ac-Leu-Cit-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 1.58 | - |
2-Naph-Lys-Orn-Arg-p-nitroanilide | - |
West Nile virus | |
| 1.6 | - |
Ac-Aib-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 1.64 | - |
Ac-Leu-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 1.74 | - |
Ac-Tbg-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 1.78 | - |
Ac-Chg-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 1.79 | - |
Ac-Leu-Orn-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 2.28 | - |
Ac-Cha-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 2.35 | - |
Ac-Nle-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 2.36 | - |
Ac-Nva-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 2.51 | - |
Ac-Aoc-Lys-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
| 118 | - |
Ac-Leu-hArg-Lys-Arg-p-nitroanilide | - |
West Nile virus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | cofactor NS2B strongly influences the preference of flavivirus proteases for lysine or arginine at P2 in substrates | West Nile virus |
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