Cloned (Comment) | Organism |
---|---|
recombinant MG 1363 strains containg plasmid-located hybrid proteinase genes constructed from SK11 and Wg2 | Lactococcus lactis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | triggers stabilization, removal of weakly bound Ca2+ from the native cell-bound enzyme is coupled with a significant reversible decrease in specific activity and a dramatic reversible reduction in thermal stability | Lactococcus lactis | |
Cd2+ | can replace Ca2+ | Lactococcus lactis | |
additional information | Mn2+ is much less efficient, Zn2+, Mg2+, Ba2+, Ni2+, Co2+, Cu2+, and Sn2+ have no significant effect | Lactococcus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
145000 | - |
Ca-free enzyme, gel filtration | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
subsp. cremoris SK11, subsp. cremoris Wg2, MG1363 | - |
Storage Stability | Organism |
---|---|
0°C, Ca-free enzyme, storage on ice results in a gradual loss of potential activity, 20% reduction in activity after 4 h | Lactococcus lactis |
Synonyms | Comment | Organism |
---|---|---|
lactococcal cell envelope proteinase | - |
Lactococcus lactis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
Ca-free cell-bound enzyme is extremely unstable, lose 99% of its potential activity within 20 min | Lactococcus lactis |