General Stability | Organism |
---|---|
stable to repeated freezing and thawing | Daboia russelii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropyl fluorophosphate | - |
Daboia russelii | |
diisopropylfluorophosphate | - |
Daboia russelii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Daboia russelii | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | mediates the interaction of factor V to factor Va | Daboia russelii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27200 | - |
x * 27200 sedimentation equilibrium centrifugation, denaturing conditions, x * 28000, SDS-PAGE, non-reducing conditions, X * 29000, SDS-PAGE, reducing conditions | Daboia russelii |
27200 | - |
1 * 27200, Vipera russelli, sedimentation equilibrium under denaturing conditions | Daboia russelii |
28000 | - |
x * 27200 sedimentation equilibrium centrifugation, denaturing conditions, x * 28000, SDS-PAGE, non-reducing conditions, X * 29000, SDS-PAGE, reducing conditions | Daboia russelii |
29000 | - |
x * 27200 sedimentation equilibrium centrifugation, denaturing conditions, x * 28000, SDS-PAGE, non-reducing conditions, X * 29000, SDS-PAGE, reducing conditions | Daboia russelii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Clotting factor V + H2O | Daboia russelii | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daboia russelii | - |
Russell's viper | - |
Daboia russelii | - |
factor V activator is separated from factor X activator by gel filtration | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 6% carbohydrate | Daboia russelii |
Purification (Comment) | Organism |
---|---|
- |
Daboia russelii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Daboia russelii | - |
venom | lyophilized | Daboia russelii | - |
Storage Stability | Organism |
---|---|
-70°C to 50°C, procoagulant activity stable | Daboia russelii |
4°C, 50 mM Tris-HCl, pH 7.5, 0.02% NaN3, protein concentration 0.5-1.5 mg/ml | Daboia russelii |
4°C, 50 mM Tris-HCl, pH 7.5, containing 0.02% NaN3 at protein concentration of 0.2 mg/ml, procoagulant activity remains constant for several months | Daboia russelii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Benzoyl-L-Arg ethyl ester + H2O | - |
Daboia russelii | Benzoyl-L-Arg + ethanol | - |
? | |
Clotting factor V + H2O | clotting factor V of human and bovine origin, no other protein substrate, not rabbit clotting factor V | Daboia russelii | Clotting factor Va | cleavage of a single peptide bond, Va is composed of a heavy chain of MW 230000 and a light chain of MW 80000 | ? | |
Clotting factor V + H2O | - |
Daboia russelii | ? | - |
? | |
Factor V + H2O | cleavage of a single internal peptide bond, human and bovine factor V, not: rabbit factor V, human or bovine factor VIII, bovine fibrinogen, bovine prothrombin | Daboia russelii | Factor Va | composed of a heavy chain, MW 230000, and a light chain, MW 80000. The heavy chain and the light chain are noncovalently associated in solution | ? | |
L-Phe-pipecolyl-L-Arg 4-nitroanilide + H2O | - |
Daboia russelii | L-Phe-pipecolyl-L-Arg + 4-nitrolaniline | - |
? | |
additional information | hydrolyzes arginine esters, such as benzoyl-Arg ethyl ester and tosyl-Arg methyl ester | Daboia russelii | ? | - |
? | |
Tosyl-L-Arg methyl ester + H2O | - |
Daboia russelii | Tosyl-L-Arg + methanol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27200 sedimentation equilibrium centrifugation, denaturing conditions, x * 28000, SDS-PAGE, non-reducing conditions, X * 29000, SDS-PAGE, reducing conditions | Daboia russelii |
monomer | 1 * 27200, Vipera russelli, sedimentation equilibrium under denaturing conditions | Daboia russelii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-70 | 50 | - |
Daboia russelii |
85 | - |
60 min, 50% loss of activity | Daboia russelii |
85 | - |
60 min, enzyme retains approximately 50% of its coagulant and esterase activity | Daboia russelii |