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Literature summary for 3.4.21.93 extracted from

  • Lloyd, D.J.; Bohan, S.; Gekakis, N.
    Obesity, hyperphagia and increased metabolic efficiency in Pc1 mutant mice (2006), Hum. Mol. Genet., 15, 1884-1893.
    View publication on PubMed
Search for more literature for 3.4.21.93

Application

Application Comment Organism
medicine Pc1 N222D homozygous mice are obese and mimic humans with mutations in PC1. This mutation will be a valuable resource in understanding the role of prohormone processing in energy homeostasis Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
human PC1 cDNA (amino acids 28-753) amplified, His-tagged and cloned into the MluI and NotI sites of a modified pFASTBAC1 expression vector. Wild-type and mutant N222D human PC1 expressed using baculovirus to infect Sf9 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
N222D autocatalytic and neuropeptide processing is impaired Homo sapiens
N222D leads to obesity, abnormal proinsulin processing, reduced fecundity, impaired autocatalysis and multiple endocrinological defects in mice homozygous for the mutation. Increased energy intake, a more efficient metabolism and reduced alpha-MSH signaling contribute to the obesity. Heterozygous littermates exhibit an intermediate phenotype for both sexes, thus this mutation results in a semi-dominant phenotype Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 Western blotting, intramolecular autocatalytic cleaved 85 kDa isoform Homo sapiens
66000
-
 Western blotting, intramolecular autocatalytically cleaved 85 kDa isoform Mus musculus
75000
-
 Western blotting, most likely a processing intermediate of PC1 Homo sapiens
85000
-
 Western blotting Mus musculus
85000
-
 Western blotting Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
 C57BL/6 mice
-
Mus musculus C57BL/6
-
 C57BL/6 mice
-

Source Tissue

Source Tissue Comment Organism Textmining
brain 66 kDa and 85kDa isoforms Mus musculus
-
hypothalamus
-
 Mus musculus
-
pancreas 66 kDa isoform Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pGlu-Arg-Thr-Lys-Arg-4-methyl-coumaryl-7-amide + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
PC1
-
 Mus musculus
PC1
-
 Homo sapiens
prohormone convertase 1
-
 Mus musculus
prohormone convertase 1
-
 Homo sapiens