Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Plasmodium falciparum | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | O97252 | proteolytic subunit | - |
Purification (Comment) | Organism |
---|---|
glutathione-sepharose 4B bead column chromatography | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-L-Arg-7-amido-4-methylcoumarin + H2O | substrate for the recombinant ClpP | Plasmodium falciparum | Gly-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
additional information | recombinant mature ClpP is not capable of hydrolyzing Gly-L-Arg-7-amido-4-methylcoumarin, GlyL-Phe-7-amido-4-methylcoumarin, and benzyloxycarbonyl-L-Gln-L-Arg-L-Arg-7-amido-4-methylcoumarin. Mature ClpP protein does not cleave N-succinyl-L-leucine-L-tyrosine-7-amido-4-methylcoumarin and N-succinyl-L-isoleucine-L-isoleucine-L-tryptophan-7-amido-4-methylcoumarin | Plasmodium falciparum | ? | - |
? | |
N-succinyl-L-isoleucine-L-isoleucine-L-tryptophan-7-amido-4-methylcoumarin + H2O | - |
Plasmodium falciparum | ? | - |
? | |
succinyl-L-Leu-L-Lys-7-amido-4-methylcoumarin + H2O | recombinant mature ClpP is most active against succinyl-L-Leu-L-Lys-7-amido-4-methylcoumarin | Plasmodium falciparum | ? | - |
? | |
succinyl-L-Leu-L-Tyr-7-amido-4-methylcoumarin + H2O | recombinant ClpP does not cleave the known ClpP substrate succinyl-L-Leu-L-Tyr-7-amido-4-methylcoumarin | Plasmodium falciparum | succinyl-L-Leu-L-Tyr + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Caseinolytic protease | - |
Plasmodium falciparum |
CLP | - |
Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Plasmodium falciparum |