Literature summary for 3.4.21.92 extracted from

Andersson, F.I.; Tryggvesson, A.; Sharon, M.; Diemand, A.V.; Classen, M.; Best, C.; Schmidt, R.; Schelin, J.; Stanne, T.M.; Bukau, B.; Robinson, C.V.; Witt, S.; Mogk, A.; Clarke, A.K.
Structure and function of a novel type of ATP-dependent CLP protease (2009), J. Biol. Chem., 284, 13519-13532.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	ClpP3/R complex stimulates the steady-state ATPase activity of ClpC	Synechococcus elongatus

Cloned(Commentary)

Cloned (Comment)	Organism
clpP3 and clpR genes cloned into the pACYC Duet vector for co-expression in Escherichia coli BL21-STAR cells	Synechococcus elongatus

Protein Variants

Protein Variants	Comment	Organism
S101A	mutation of the active site Ser-101 in ClpP3 inactivates the entire ClpCP3/R protease, the mutation has no effect on the formation of the recombinant ClpP3/R core complex of 270 kDa. The mutated ClpP3/R complex stimulates the steady-state ATPase activity of ClpC to the same extent as wild-type ClpP3/R. The mutated ClpP3/R core fails to degrade the alpha-casein nor the more sensitive FITC-casein	Synechococcus elongatus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
270000	-	native-PAGE	Synechococcus elongatus

Organism

Organism	UniProt	Comment	Textmining
Synechococcus elongatus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ClpP3/R complex purified on Ni2+ affinity column and by gel filtration, purification of ClpC and ClpS1	Synechococcus elongatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-casein + H2O	is completely degraded by ClpC and ClpP3/R within 20 min	Synechococcus elongatus	?	-	?
FITC-casein + H2O	neither ClpC nor ClpP3/R alone degrade FITC-casein but they do when added together. No proteolytic activity when ClpP3 alone is combined with ClpC	Synechococcus elongatus	?	-	?
FR-GFP + H2O	ClpCP3/R with ClpS1 take over 20 min to completely degrade FR-GFP, whereas the ClpAP protease degrades all FR-GFP within 2 min	Synechococcus elongatus	?	-	?
additional information	ClpR subunit is proteolytically inactive, thus ClpR subunit does not contribute to the proteolytic activity of the ClpP3/R core. Inclusion of ClpR is not rate-limiting for the ClpCP3/R protease. ClpC is not affected by auto-degradation as is ClpA. ClpS1 alters the substrate specificity of the ClpCP3/R protease	Synechococcus elongatus	?	-	?
N-succinyl-Ile-Ile-Trp-7-amido-4-methylcoumarin + H2O	throughout the 5 min time course, ClpP readily degrades the dipeptide, whereas ClpP3/R does not. Prolonging the incubation time with ClpP3/R to 20 min does not result in any visible degradation. Addition of ClpC to the assays also fails to produce any degradation	Synechococcus elongatus	N-succinyl-Ile-Ile-Trp + 7-amino-4-methylcoumarin	-	?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O	throughout the 5 min time course, ClpP readily degrades the dipeptide, whereas ClpP3/R does not. Prolonging the incubation time with ClpP3/R to 20 min does not result in any visible degradation. Addition of ClpC to the assays also fails to produce any degradation	Synechococcus elongatus	N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin	-	?
N-succinyl-Val-Lys-Met-7-amido-4-methylcoumarin + H2O	throughout the 5 min time course, ClpP readily degrades the dipeptide, whereas ClpP3/R does not. Prolonging the incubation time with ClpP3/R to 20 min does not result in any visible degradation. Addition of ClpC to the assays also fails to produce any degradation	Synechococcus elongatus	N-succinyl-Val-Lys-Met + 7-amino-4-methylcoumarin	-	?

Subunits

Subunits	Comment	Organism
oligomer	ClpP3/R complex, SDS-PAGE	Synechococcus elongatus

Synonyms

Synonyms	Comment	Organism
Clp protease	-	Synechococcus elongatus
ClpA	-	Synechococcus elongatus
ClpC	-	Synechococcus elongatus
ClpCP3/R protease	-	Synechococcus elongatus
ClpP	-	Synechococcus elongatus
ClpP3	-	Synechococcus elongatus
ClpP3/R complex	-	Synechococcus elongatus
ClpR	-	Synechococcus elongatus
ClpS1	-	Synechococcus elongatus

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Release 2023.1 (January 2023)