BRENDA - Enzyme Database
show all sequences of 3.4.21.92

Endopeptidase Clp: ATP-dependent Clp protease from Escherichia coli

Maurizi, M.R.; Thompson, M.W.; Singh, S.K.; Kim, S.H.; Methods Enzymol. 244, 314-331 (1994)

Data extracted from this reference:

General Stability
General Stability
Organism
ATP and nonhydrolyzable analogs of ATP stabilize subunit ClpA
Escherichia coli
Dilution, particularly in plastic tubes inactivates, stabilization by including 0.05% v/v Triton X-100 and 0.1 M KCl stabilizes subunit ClpA
Escherichia coli
In buffers containing Triton X-100, subunit ClpA is stable for several days
Escherichia coli
Repeated freezing and thawing leads to loss of activity of subunit ClpA
Escherichia coli
Subunit ClpA is sensitive to freezing
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
diisopropyl fluorophosphate
inhibits both oligopeptidase activity of ClpP and proteinase activity of ClpAP
Escherichia coli
fluorosulfonylbenzoyladenosine
-
Escherichia coli
High salt concentrations
chloride is much more inhibitory than acetate, divalent anions are also very inhibitory
Escherichia coli
kappa-casein
strong, competitive
Escherichia coli
Mg2+
proteolytic activity of ClpAP is dependent on, but concentrations higher than about 30 mM are inhibitory
Escherichia coli
Xaa-Tyr-Leu-Tyr-Trp
competitive to succinyl-Leu-Tyr 4-methylcoumarin 7-amide degradation
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
poor substitute for Mg2+
Escherichia coli
Mg2+
proteolytic activity of ClpAP is dependent on, concentrations higher than about 30 mM are inhibitory
Escherichia coli
Zn2+
poor substitute for Mg2+
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
21000
-
1 * 230000, subunit ClpP (12 * 21000, amino acid sequence, subunit of ClpP)
Escherichia coli
83000
-
x * 120000-140000, subunit ClpA, gel filtration, x * 83000, subunit ClpA, amino acid sequence
Escherichia coli
230000
-
1 * 230000, subunit ClpP (12 * 21000, amino acid sequence, subunit of ClpP)
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Escherichia coli
ClpXP appears to be involved in plasmid maintenance and in phage Mu virulence
?
-
-
-
additional information
Escherichia coli
the high degree of similarity among the ClpA-like proteins suggests that Clp-like proteases are likely to be important participants in energy-dependent proteolysis in prokaryotic and eukaryotic cells
?
-
-
-
additional information
Escherichia coli CSH100 (ClpA)
ClpXP appears to be involved in plasmid maintenance and in phage Mu virulence
?
-
-
-
additional information
Escherichia coli CSH100 (ClpA)
the high degree of similarity among the ClpA-like proteins suggests that Clp-like proteases are likely to be important participants in energy-dependent proteolysis in prokaryotic and eukaryotic cells
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
CSH100 (ClpA); wild-type Escherichia coli cells transformed with the multicopy plasmid pWPC9 (ClpP)
-
Escherichia coli CSH100 (ClpA)
-
CSH100 (ClpA)
-
Purification (Commentary)
Purification (Commentary)
Organism
ClpA; ClpP
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Escherichia coli
Storage Stability
Storage Stability
Organism
-70°C, 50 mM Tris-HCl, pH 7.5, 2 mM EDTA, 2 mM DTT, 10% v/v glycerol, stable for extended periods
Escherichia coli
-70°C, ClpP stable
Escherichia coli
-70°C, subunit ClpP stable
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
Bacteriophage lambdaO-DNA replication protein + H2O
degraded by ClpXP
29858
Escherichia coli
Hydrolyzed bacteriophage lambdaO-DNA replication protein
-
-
-
-
beta-Galactosidase fusion proteins + H2O
-
29858
Escherichia coli
Hydrolyzed beta-galactosidase fusion protein
-
-
-
-
casein + H2O
alpha-casein
29858
Escherichia coli
small peptides derived from casein
-
29858
Escherichia coli
-
Glucagon + H2O
cleavage at multiple sites
29858
Escherichia coli
Hydrolyzed glucagon
-
-
-
-
Glucagon + H2O
cleavage at multiple sites
29858
Escherichia coli CSH100 (ClpA)
Hydrolyzed glucagon
-
-
-
-
Leu-Tyr-Leu-Tyr-Trp + H2O
cleavage occurs primarily at Leu3-Tyr4, but significant cleavage also at Tyr2-Leu3 and Leu4-Trp5 bond
29858
Escherichia coli
Leu-Tyr-Leu + Tyr-Trp
-
29858
Escherichia coli
-
additional information
ClpP subunit has peptidase activity against very short peptides, with fewer than five amino acid residues in the absence of ClpA and nucleotide
29858
Escherichia coli
?
-
-
-
-
additional information
when activated by ClpA subunit, ClpP can degrade longer polypeptides and proteins
29858
Escherichia coli
?
-
-
-
-
additional information
ClpXP appears to be involved in plasmid maintenance and in phage Mu virulence
29858
Escherichia coli
?
-
-
-
-
additional information
the high degree of similarity among the ClpA-like proteins suggests that Clp-like proteases are likely to be important participants in energy-dependent proteolysis in prokaryotic and eukaryotic cells
29858
Escherichia coli
?
-
-
-
-
additional information
ClpP subunit has peptidase activity against very short peptides, with fewer than five amino acid residues in the absence of ClpA and nucleotide
29858
Escherichia coli CSH100 (ClpA)
?
-
-
-
-
additional information
when activated by ClpA subunit, ClpP can degrade longer polypeptides and proteins
29858
Escherichia coli CSH100 (ClpA)
?
-
-
-
-
additional information
ClpXP appears to be involved in plasmid maintenance and in phage Mu virulence
29858
Escherichia coli CSH100 (ClpA)
?
-
-
-
-
additional information
the high degree of similarity among the ClpA-like proteins suggests that Clp-like proteases are likely to be important participants in energy-dependent proteolysis in prokaryotic and eukaryotic cells
29858
Escherichia coli CSH100 (ClpA)
?
-
-
-
-
Oxidized insulin B-chain + H2O
cleavage at multiple sites
29858
Escherichia coli
Hydrolyzed insulin B-chain
-
-
-
-
Phe-Ala-Pro-His-Met-Ala-Leu-Val-Pro-Val + H2O
synthetic polypeptide that corresponds to the 10 amino acids surrounding the in vivo processing site in ClpP subunit
29858
Escherichia coli
?
-
-
-
-
Succinyl-Ala-Ala-Phe 4-methylcoumarin 7-amide + H2O
ClpP subunit alone
29858
Escherichia coli
Succinyl-Ala-Ala + Phe 4-methylcoumarin 7-amide
-
29858
Escherichia coli
-
Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O
ClpP subunit alone
29858
Escherichia coli
Succinyl-Leu + Leu + Val-Tyr 4-methylcoumarin 7-amide
-
29858
Escherichia coli
-
Succinyl-Leu-Tyr 4-methylcoumarin 7-amide + H2O
ClpP subunit alone
29858
Escherichia coli
Succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
29858
Escherichia coli
-
Subunits
Subunits
Commentary
Organism
?
1 * 230000, subunit ClpP (12 * 21000, amino acid sequence, subunit of ClpP); ClpP is composed of two superimposed rings of six subunits each, ClpA and ClpP form a tight complex in the presence of MgCl2 and ATP, the ClpAP complex is composed of a dodecamer of ClpP and a hexamer of ClpA; x * 120000-140000, subunit ClpA, gel filtration, x * 83000, subunit ClpA, amino acid sequence
Escherichia coli
More
bacteria, tomatoes and Trypanosomes all contain genes for a large protein with extensive homology to the regulartory subunit, ClpA; but the combination of ClpX and ClpP has very little activity against alpha-casein; ClpP and ClpA interact to form the active protease, this complex degrades a number of proteins, such as alpha-casein into small peptides and can hydrolyze ATP; ClpX participates with ClpP in the rapid and specific degradation of the lambda O protein; enzyme consists of two components: ClpP and ClpA or ClpX
Escherichia coli
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
42
-
active in degrading alpha-casein up to 42°C, no proteinase activity at 55°C
Escherichia coli
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
10
-
inactivation after a short time
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.5
-
glucagon
value below
Escherichia coli
0.5
-
oxidized insulin B-chain
value below
Escherichia coli
1.17
-
ATP
ATPase activity
Escherichia coli
2.17
-
ATP
proteolysis
Escherichia coli
13.3
-
Phe-Ala-Pro-His-Met-Ala-Leu-Val-Pro-Val
-
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
6.3 (ClpA)
Escherichia coli
7
-
succinyl-Leu-Tyr 4-methylcoumarin 7-amide
Escherichia coli
7.5
9.5
alpha-casein
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
cleavage of peptides of more than five amino acids residues by ClpP requires activation by ClpA and either ATP or a nonhydrolyzable analog of ATP such as ATPgammaS or 5'-adenylyl imidodiphosphate; only ATP and dATP support alpha-casein degradation
Escherichia coli
dATP
only ATP and dATP support alpha-casein degradation
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
cleavage of peptides of more than five amino acids residues by ClpP requires activation by ClpA and either ATP or a nonhydrolyzable analog of ATP such as ATPgammaS or 5'-adenylyl imidodiphosphate; only ATP and dATP support alpha-casein degradation
Escherichia coli
dATP
only ATP and dATP support alpha-casein degradation
Escherichia coli
General Stability (protein specific)
General Stability
Organism
ATP and nonhydrolyzable analogs of ATP stabilize subunit ClpA
Escherichia coli
Dilution, particularly in plastic tubes inactivates, stabilization by including 0.05% v/v Triton X-100 and 0.1 M KCl stabilizes subunit ClpA
Escherichia coli
In buffers containing Triton X-100, subunit ClpA is stable for several days
Escherichia coli
Repeated freezing and thawing leads to loss of activity of subunit ClpA
Escherichia coli
Subunit ClpA is sensitive to freezing
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
diisopropyl fluorophosphate
inhibits both oligopeptidase activity of ClpP and proteinase activity of ClpAP
Escherichia coli
fluorosulfonylbenzoyladenosine
-
Escherichia coli
High salt concentrations
chloride is much more inhibitory than acetate, divalent anions are also very inhibitory
Escherichia coli
kappa-casein
strong, competitive
Escherichia coli
Mg2+
proteolytic activity of ClpAP is dependent on, but concentrations higher than about 30 mM are inhibitory
Escherichia coli
Xaa-Tyr-Leu-Tyr-Trp
competitive to succinyl-Leu-Tyr 4-methylcoumarin 7-amide degradation
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
poor substitute for Mg2+
Escherichia coli
Mg2+
proteolytic activity of ClpAP is dependent on, concentrations higher than about 30 mM are inhibitory
Escherichia coli
Zn2+
poor substitute for Mg2+
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
21000
-
1 * 230000, subunit ClpP (12 * 21000, amino acid sequence, subunit of ClpP)
Escherichia coli
83000
-
x * 120000-140000, subunit ClpA, gel filtration, x * 83000, subunit ClpA, amino acid sequence
Escherichia coli
230000
-
1 * 230000, subunit ClpP (12 * 21000, amino acid sequence, subunit of ClpP)
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Escherichia coli
ClpXP appears to be involved in plasmid maintenance and in phage Mu virulence
?
-
-
-
additional information
Escherichia coli
the high degree of similarity among the ClpA-like proteins suggests that Clp-like proteases are likely to be important participants in energy-dependent proteolysis in prokaryotic and eukaryotic cells
?
-
-
-
additional information
Escherichia coli CSH100 (ClpA)
ClpXP appears to be involved in plasmid maintenance and in phage Mu virulence
?
-
-
-
additional information
Escherichia coli CSH100 (ClpA)
the high degree of similarity among the ClpA-like proteins suggests that Clp-like proteases are likely to be important participants in energy-dependent proteolysis in prokaryotic and eukaryotic cells
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
ClpA; ClpP
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
-70°C, 50 mM Tris-HCl, pH 7.5, 2 mM EDTA, 2 mM DTT, 10% v/v glycerol, stable for extended periods
Escherichia coli
-70°C, ClpP stable
Escherichia coli
-70°C, subunit ClpP stable
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
Bacteriophage lambdaO-DNA replication protein + H2O
degraded by ClpXP
29858
Escherichia coli
Hydrolyzed bacteriophage lambdaO-DNA replication protein
-
-
-
-
beta-Galactosidase fusion proteins + H2O
-
29858
Escherichia coli
Hydrolyzed beta-galactosidase fusion protein
-
-
-
-
casein + H2O
alpha-casein
29858
Escherichia coli
small peptides derived from casein
-
29858
Escherichia coli
-
Glucagon + H2O
cleavage at multiple sites
29858
Escherichia coli
Hydrolyzed glucagon
-
-
-
-
Glucagon + H2O
cleavage at multiple sites
29858
Escherichia coli CSH100 (ClpA)
Hydrolyzed glucagon
-
-
-
-
Leu-Tyr-Leu-Tyr-Trp + H2O
cleavage occurs primarily at Leu3-Tyr4, but significant cleavage also at Tyr2-Leu3 and Leu4-Trp5 bond
29858
Escherichia coli
Leu-Tyr-Leu + Tyr-Trp
-
29858
Escherichia coli
-
additional information
ClpP subunit has peptidase activity against very short peptides, with fewer than five amino acid residues in the absence of ClpA and nucleotide
29858
Escherichia coli
?
-
-
-
-
additional information
when activated by ClpA subunit, ClpP can degrade longer polypeptides and proteins
29858
Escherichia coli
?
-
-
-
-
additional information
ClpXP appears to be involved in plasmid maintenance and in phage Mu virulence
29858
Escherichia coli
?
-
-
-
-
additional information
the high degree of similarity among the ClpA-like proteins suggests that Clp-like proteases are likely to be important participants in energy-dependent proteolysis in prokaryotic and eukaryotic cells
29858
Escherichia coli
?
-
-
-
-
additional information
ClpP subunit has peptidase activity against very short peptides, with fewer than five amino acid residues in the absence of ClpA and nucleotide
29858
Escherichia coli CSH100 (ClpA)
?
-
-
-
-
additional information
when activated by ClpA subunit, ClpP can degrade longer polypeptides and proteins
29858
Escherichia coli CSH100 (ClpA)
?
-
-
-
-
additional information
ClpXP appears to be involved in plasmid maintenance and in phage Mu virulence
29858
Escherichia coli CSH100 (ClpA)
?
-
-
-
-
additional information
the high degree of similarity among the ClpA-like proteins suggests that Clp-like proteases are likely to be important participants in energy-dependent proteolysis in prokaryotic and eukaryotic cells
29858
Escherichia coli CSH100 (ClpA)
?
-
-
-
-
Oxidized insulin B-chain + H2O
cleavage at multiple sites
29858
Escherichia coli
Hydrolyzed insulin B-chain
-
-
-
-
Phe-Ala-Pro-His-Met-Ala-Leu-Val-Pro-Val + H2O
synthetic polypeptide that corresponds to the 10 amino acids surrounding the in vivo processing site in ClpP subunit
29858
Escherichia coli
?
-
-
-
-
Succinyl-Ala-Ala-Phe 4-methylcoumarin 7-amide + H2O
ClpP subunit alone
29858
Escherichia coli
Succinyl-Ala-Ala + Phe 4-methylcoumarin 7-amide
-
29858
Escherichia coli
-
Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O
ClpP subunit alone
29858
Escherichia coli
Succinyl-Leu + Leu + Val-Tyr 4-methylcoumarin 7-amide
-
29858
Escherichia coli
-
Succinyl-Leu-Tyr 4-methylcoumarin 7-amide + H2O
ClpP subunit alone
29858
Escherichia coli
Succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
29858
Escherichia coli
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
1 * 230000, subunit ClpP (12 * 21000, amino acid sequence, subunit of ClpP); ClpP is composed of two superimposed rings of six subunits each, ClpA and ClpP form a tight complex in the presence of MgCl2 and ATP, the ClpAP complex is composed of a dodecamer of ClpP and a hexamer of ClpA; x * 120000-140000, subunit ClpA, gel filtration, x * 83000, subunit ClpA, amino acid sequence
Escherichia coli
More
bacteria, tomatoes and Trypanosomes all contain genes for a large protein with extensive homology to the regulartory subunit, ClpA; but the combination of ClpX and ClpP has very little activity against alpha-casein; ClpP and ClpA interact to form the active protease, this complex degrades a number of proteins, such as alpha-casein into small peptides and can hydrolyze ATP; ClpX participates with ClpP in the rapid and specific degradation of the lambda O protein; enzyme consists of two components: ClpP and ClpA or ClpX
Escherichia coli
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
42
-
active in degrading alpha-casein up to 42°C, no proteinase activity at 55°C
Escherichia coli
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
10
-
inactivation after a short time
Escherichia coli
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.5
-
glucagon
value below
Escherichia coli
0.5
-
oxidized insulin B-chain
value below
Escherichia coli
1.17
-
ATP
ATPase activity
Escherichia coli
2.17
-
ATP
proteolysis
Escherichia coli
13.3
-
Phe-Ala-Pro-His-Met-Ala-Leu-Val-Pro-Val
-
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
6.3 (ClpA)
Escherichia coli
7
-
succinyl-Leu-Tyr 4-methylcoumarin 7-amide
Escherichia coli
7.5
9.5
alpha-casein
Escherichia coli
Other publictions for EC 3.4.21.92
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752509
Choules
Rufomycin targets ClpC1 prote ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Antimicrob. Agents Chemother.
63
e02204
2019
-
-
-
-
1
-
1
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
755519
Sivertsson
The AAA+ protease ClpXP can e ...
Escherichia coli
Sci. Rep.
9
2421
2019
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
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-
-
1
-
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1
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3
-
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-
-
1
-
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-
-
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-
753915
Marsee
Mycobacterium tuberculosis Cl ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Int. J. Mol. Sci.
19
3651
2018
-
-
-
-
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-
1
-
-
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5
-
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1
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1
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1
1
-
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-
754176
Weinhaeupl
The antibiotic cyclomarin blo ...
Mycobacterium tuberculosis
J. Biol. Chem.
293
8379-8393
2018
-
-
-
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1
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-
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2
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1
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-
-
1
1
-
-
-
755502
Shi
Optimization of ClpXP activit ...
Escherichia coli
Sci. Rep.
8
3488
2018
-
1
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
752357
Amor
Highly dynamic interactions m ...
Escherichia coli
ACS Chem. Biol.
11
1552-1560
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
752359
Ni
Characterization of gain-of-f ...
Staphylococcus aureus, Staphylococcus aureus MW2
ACS Chem. Biol.
11
1964-1972
2016
-
-
-
1
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
752813
Zhang
Mitochondrial ClpP activity i ...
Homo sapiens
Biochim. Biophys. Acta
1862
252-264
2016
-
1
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
754888
Trentini
Arginine phosphorylation mark ...
Bacillus subtilis 168, Bacillus subtilis
Nature
539
48-53
2016
-
-
-
-
1
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
732563
Gersch
AAA+ chaperones and acyldepsip ...
Staphylococcus aureus, Staphylococcus aureus NCTC 8325
Nat. Commun.
6
6320
2015
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
752706
Aguado
ClpB dynamics is driven by it ...
Escherichia coli, Escherichia coli BB4561
Biochem. J.
466
561-570
2015
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
754914
Tapken
The Clp protease system is re ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
New Phytol.
205
511-517
2015
-
-
-
-
-
-
-
-
1
-
-
-
-
27
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
755086
Leodolter
The Mycobacterium tuberculosi ...
Mycobacterium tuberculosis
PLoS ONE
10
e0125345
2015
-
-
-
-
2
-
-
-
-
-
1
-
-
4
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
731637
Cordova
Stochastic but highly coordina ...
Escherichia coli
Cell
158
647-658
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
731945
Li
Cloning and characterization o ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Int. J. Clin. Exp. Pathol.
7
5674-5682
2014
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
731985
Gersch
Disruption of oligomerization ...
Staphylococcus aureus
J. Am. Chem. Soc.
136
1360-1366
2014
-
-
-
-
-
-
8
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
8
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
732009
Wahl
Control of natural transformat ...
Streptococcus thermophilus, Streptococcus thermophilus ATCC BAA-250
J. Bacteriol.
196
2807-2816
2014
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
732159
Sato
FliT selectively enhances prot ...
Salmonella enterica, Salmonella enterica ATCC 700720
J. Biol. Chem.
289
33001-33011
2014
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
732169
Brzozowska
The ClpXP protease is responsi ...
Bacillus subtilis
J. Biol. Chem.
289
7514-7523
2014
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
731636
Sen
-
The ClpXP protease unfolds sub ...
Escherichia coli
Cell
155
X636-X646
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
731815
Bonnet
FixK2, a key regulator in Brad ...
Bradyrhizobium japonicum
FEBS Lett.
587
88-93
2013
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
732530
Bhat
Identification of ClpP substra ...
Caulobacter vibrioides
Mol. Microbiol.
88
1083-1092
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
732717
Xie
The ClpP protease is required ...
Actinobacillus pleuropneumoniae
PLoS ONE
8
e53600
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
732721
Personne
Mycobacterium tuberculosis Clp ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
PLoS ONE
8
e60228
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
12
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
732950
Lee
Streptococcus pneumoniae ClpP ...
Streptococcus pneumoniae
Toxicon
70
142-152
2013
-
1
1
-
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717738
Ollinger
Validation of the essential Cl ...
Mycobacterium tuberculosis
J. Bacteriol.
194
663-668
2012
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
731272
Tryggvesson
Interaction specificity betwee ...
Synechococcus elongatus, Synechococcus elongatus PCC 7942
Biochem. J.
446
311-320
2012
-
-
-
1
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
732638
Derrien
The purification of the Chlamy ...
Chlamydomonas reinhardtii
Plant Mol. Biol.
80
189-202
2012
-
-
-
-
-
-
-
-
1
-
-
-
-
7
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734574
Alexopoulos
ClpP: a structurally dynamic p ...
Bacillus subtilis, Escherichia coli
J. Struct. Biol.
179
202-210
2012
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
4
2
6
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717089
Geiger
A conformational switch underl ...
Staphylococcus aureus
Angew. Chem. Int. Ed. Engl.
50
5749-5752
2011
-
-
-
1
10
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
10
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
717472
Maillard
ClpX(P) generates mechanical f ...
Escherichia coli
Cell
145
459-469
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
717708
Religa
Site-directed methyl group lab ...
Escherichia coli
J. Am. Chem. Soc.
133
9063-9068
2011
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717731
Camberg
The interplay of ClpXP with th ...
Escherichia coli
J. Bacteriol.
193
1911-1918
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
717999
Nager
Stepwise unfolding of a beta-b ...
Escherichia coli
J. Mol. Biol.
413
4-16
2011
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
718088
Kajfasz
Transcriptome analysis reveals ...
Streptococcus mutans
Microbiology
157
2880-2890
2011
-
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718089
Kitagawa
Dual regulatory pathways of fl ...
Escherichia coli
Microbiology
157
3094-3103
2011
-
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2
2
-
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718090
Cohn
Clp-dependent proteolysis of t ...
Staphylococcus aureus
Microbiology
157
677-684
2011
-
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1
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1
1
-
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-
718114
Lee
Structural insights into the c ...
Bacillus subtilis
Mol. Cells
32
589-595
2011
-
-
1
1
-
-
1
-
-
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-
718131
Martinez-Noel
NifB and NifEN protein levels ...
Azotobacter vinelandii
Mol. Microbiol.
79
1182-1193
2011
-
-
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-
-
1
1
-
-
-
718212
Sjoegren
Assembly of the chloroplast AT ...
Arabidopsis thaliana
Plant Cell
23
322-332
2011
-
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4
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-
-
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-
-
-
-
-
1
1
-
-
-
717092
Wu
ClpC1, an ATP-dependent Clp pr ...
Arabidopsis thaliana
Ann. Bot.
105
823-833
2010
-
-
-
-
1
-
-
-
1
-
-
-
-
4
-
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-
-
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-
-
2
2
-
-
-
717787
Effantin
Binding of the ClpA unfoldase ...
Escherichia coli
J. Biol. Chem.
285
14834-14840
2010
-
-
-
1
-
-
-
-
-
-
-
1
-
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-
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-
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-
-
-
-
-
-
-
-
-
717989
Lee
Control of substrate gating an ...
Escherichia coli
J. Mol. Biol.
399
707-718
2010
1
-
1
-
16
-
-
-
-
-
-
1
-
1
-
-
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4
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1
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-
-
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-
717993
El Bakkouri
The Clp chaperones and proteas ...
Plasmodium falciparum
J. Mol. Biol.
404
456-477
2010
-
-
-
1
-
-
-
1
1
-
2
-
-
8
-
-
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-
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2
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6
2
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1
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-
718140
Li
ClpXP protease regulates the t ...
Dickeya dadantii
Mol. Plant Microbe Interact.
23
871-878
2010
-
-
-
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-
-
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-
-
-
-
-
3
-
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1
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1
1
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-
718406
Kimber
Structural and theoretical stu ...
Escherichia coli
Structure
18
798-808
2010
-
-
-
-
1
-
-
3
-
-
2
-
-
6
-
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-
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1
1
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3
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-
-
-
696989
Jana
Mechanism of protonophores-med ...
Escherichia coli
BMC Microbiol.
9
20
2009
1
-
-
-
1
-
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-
-
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1
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-
-
-
-
-
-
-
-
-
-
-
697889
Guillon
Frataxin deficiency causes upr ...
Mus musculus
FEBS J.
276
1036-1047
2009
2
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698609
de Bruijn
Regulation of cyclic lipopepti ...
Pseudomonas fluorescens, Pseudomonas fluorescens SS101
J. Bacteriol.
191
1910-1923
2009
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
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-
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698890
Andersson
Structure and function of a no ...
Synechococcus elongatus
J. Biol. Chem.
284
13519-13532
2009
1
-
1
-
1
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
-
-
7
1
9
-
-
-
-
-
-
-
-
-
-
-
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-
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-
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-
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-
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-
-
-
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700285
Hahn
McsA and B mediate the delocal ...
Bacillus subtilis
Mol. Microbiol.
72
202-215
2009
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
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-
-
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-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
701368
Cao
CD4(+) T lymphocytes mediated ...
Streptococcus pneumoniae TIGR4
Vaccine
27
2838-2844
2009
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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-
-
-
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-
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-
-
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-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701378
Gumber
Evaluation of the immunogenici ...
Mycobacterium avium subsp. paratuberculosis
Vet. Microbiol.
137
290-296
2009
-
1
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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1
-
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-
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-
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-
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-
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1
-
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710200
Lin
Atypical caseinolytic protease ...
Plasmodium falciparum
Parasitol. Res.
105
1715-1722
2009
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
1
-
-
-
-
-
5
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
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-
-
-
-
-
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1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696224
Jennings
The ClpP N-terminus coordinate ...
Escherichia coli
Biochemistry
47
11031-11040
2008
-
-
1
-
1
-
-
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
4
-
3
-
-
-
3
-
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-
-
-
-
-
-
-
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-
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-
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-
-
4
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
698464
Boettcher
Beta-lactones as specific inhi ...
Staphylococcus aureus
J. Am. Chem. Soc.
130
14400-14401
2008
-
1
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
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1
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3
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699539
Kim
The structural basis for the a ...
Helicobacter pylori
J. Mol. Biol.
379
760-771
2008
-
-
1
1
1
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
1
1
-
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-
-
-
-
-
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-
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-
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1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700017
Cao
Mucosal immunization with puri ...
Streptococcus pneumoniae TIGR4
Microbes Infect.
10
1536-1542
2008
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700468
Hansen
Decreased expression of the mi ...
Homo sapiens
Neuroscience
153
474-482
2008
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
3
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676527
Nakagawara
Clp protease controls chloroph ...
Arabidopsis thaliana
Plant J.
49
800-809
2007
-
-
-
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677704
Cohn
Contribution of conserved ATP- ...
Campylobacter jejuni, Campylobacter jejuni NCTC 11168
Appl. Environ. Microbiol.
73
7803-7813
2007
-
1
-
-
-
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683473
Yu
ClpP: a distinctive family of ...
Escherichia coli, Homo sapiens, Mycobacterium tuberculosis, Plasmodium falciparum, Streptococcus pneumoniae
FEBS Lett.
581
3749-3757
2007
1
4
-
5
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
5
6
-
-
-
-
-
-
-
-
-
-
-
1
4
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683643
Stanne
Distinctive types of ATP-depen ...
Synechococcus elongatus
J. Biol. Chem.
282
14394-14402
2007
-
-
-
-
-
-
-
-
2
-
3
-
-
8
-
-
1
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683865
Farrell
Altered specificity of a AAA+ ...
Escherichia coli
Mol. Cell
25
161-166
2007
-
-
1
-
4
-
2
10
-
-
-
-
-
1
-
-
1
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
2
-
10
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683866
Prepiak
A peptide signal for adapter p ...
Bacillus subtilis
Mol. Cell
26
639-647
2007
-
-
-
-
-
-
-
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-
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1
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-
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-
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683899
Frees
Clp ATPases and ClpP proteolyt ...
Bacillus subtilis
Mol. Microbiol.
63
1285-1295
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
8
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670535
Rudella
Downregulation of ClpR2 leads ...
Arabidopsis thaliana
Plant Cell
18
1704-1721
2006
-
-
-
-
1
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
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1
-
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-
-
-
-
-
-
1
-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670633
Zheng
A nuclear-encoded ClpP subunit ...
Arabidopsis thaliana
Planta
224
1103-1115
2006
-
-
-
-
1
-
-
-
3
-
-
-
-
3
-
-
-
-
-
-
-
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-
-
-
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-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683601
Michel
Global regulatory impact of Cl ...
Staphylococcus aureus
J. Bacteriol.
188
5783-5796
2006
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
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-
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1
-
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-
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-
-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683895
Zellmeier
Involvement of Clp protease ac ...
Bacillus subtilis
Mol. Microbiol.
61
1569-1582
2006
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683977
Sjoegren
Structural and functional insi ...
Arabidopsis thaliana
Plant Cell
18
2635-2649
2006
-
-
-
-
-
-
-
-
2
-
3
-
-
5
-
-
-
-
-
1
-
-
3
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667341
Barker-Astrom
Chlorosis during nitrogen star ...
Synechococcus elongatus
Arch. Microbiol.
183
66-69
2005
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667634
Choi
Control of peptide product siz ...
Escherichia coli
Biochemistry
44
13921-13931
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668927
Ibrahim
Contribution of the ATP-depend ...
Streptococcus pneumoniae
Infect. Immun.
73
730-740
2005
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669324
Gribun
The ClpP double ring tetradeca ...
Streptococcus pneumoniae
J. Biol. Chem.
280
16185-16196
2005
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669410
Hinnerwisch
Roles of the N-domains of the ...
Escherichia coli
J. Biol. Chem.
280
40838-40844
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669088
Kock
The ClpP peptidase is the majo ...
Bacillus subtilis
J. Bacteriol.
186
5856-5864
2004
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
652115
Peltier
Identification of a 350-kDa Cl ...
Arabidopsis thaliana
J. Biol. Chem.
276
16318-16327
2001
-
-
-
-
-
-
-
-
2
-
7
-
-
3
-
-
1
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
7
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653568
Halperin
ATP-dependent association betw ...
Pisum sativum
Planta
213
614-619
2001
-
-
-
-
-
-
-
-
2
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653668
Ishikawa
Translocation pathway of prote ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
98
4328-4333
2001
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
2
-
-
-
-
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-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653260
Porankiewicz
New insights into the ATP-depe ...
Aquifex aeolicus, Arabidopsis thaliana, Bacillus subtilis, Bordetella pertussis, Borreliella burgdorferi, Caenorhabditis elegans, Caulobacter vibrioides, Chlamydia trachomatis, Chlamydomonas moewusii, Chlamydomonas reinhardtii, Chlorella vulgaris, Chlorobaculum tepidum, Clostridium acetobutylicum, Cyanophora paradoxa, Deinococcus radiodurans, Enterococcus faecalis, Epifagus virginiana, Escherichia coli, Fritillaria agrestis, Haemophilus influenzae, Helicobacter pylori, Homo sapiens, Lactococcus lactis, Listeria monocytogenes, Marchantia polymorpha, Mus musculus, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Myxococcus xanthus, Nicotiana tabacum, Oryza sativa, Paracoccus denitrificans, Pinus contorta, Pinus thunbergii, Populus tremula, Porphyromonas gingivalis, Pseudomonas aeruginosa, Rhodobacter capsulatus, Salmonella enterica subsp. enterica serovar Typhimurium, Shewanella putrefaciens, Solanum lycopersicum, Streptococcus pyogenes, Streptococcus salivarius, Streptomyces coelicolor, Synechococcus sp., Synechocystis sp., Treponema pallidum, Triticum aestivum, Yersinia enterocolitica, Zea mays
Mol. Microbiol.
32
449-458
1999
-
-
46
1
-
-
-
-
16
1
-
2
-
62
-
-
-
-
-
-
-
-
2
-
52
-
-
-
-
-
-
-
3
-
-
-
-
-
47
3
1
-
-
-
-
-
-
16
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653631
Smith
Lon and Clp family proteases a ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
96
6678-6682
1999
-
-
1
-
-
-
-
-
-
-
4
-
-
1
-
-
1
-
-
-
-
-
-
1
5
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29858
Maurizi
Endopeptidase Clp: ATP-depende ...
Escherichia coli, Escherichia coli CSH100 (ClpA)
Methods Enzymol.
244
314-331
1994
-
-
-
-
-
5
6
-
-
3
3
4
-
3
-
-
1
-
-
-
1
3
19
2
-
-
1
1
5
3
-
-
2
-
-
-
-
-
-
2
-
-
5
-
6
-
-
-
3
3
4
-
-
-
1
-
-
1
3
19
2
-
1
1
5
3
-
-
-
-
-
-
-
-
-
29863
Damerau
Role of Clp protease subunits ...
Escherichia coli
J. Bacteriol.
175
53-63
1993
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29865
Wojtkowiak
Isolation and characterization ...
Escherichia coli, Escherichia coli W3110 B178
J. Biol. Chem.
268
22609-22617
1993
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
1
2
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
2
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29866
Gottesman
ClpX, an alternative subunit f ...
Escherichia coli
J. Biol. Chem.
268
22618-22626
1993
-
-
1
-
-
-
-
-
-
-
1
1
-
2
-
-
1
-
-
-
1
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
1
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29870
Shapito
A role for the Clp protease in ...
Escherichia coli
J. Bacteriol.
175
2625-2631
1993
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29869
Geuskens
Virulence in bacteriophage Mu: ...
Escherichia coli
EMBO J.
11
5121-5127
1992
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29867
Maurizi
ATP-promoted interaction betwe ...
Escherichia coli
Biochem. Soc. Trans.
19
719-723
1991
-
-
-
-
-
-
-
-
2
-
1
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29856
Gottesman
The ATP-dependent Clp protease ...
Escherichia coli
J. Biol. Chem.
265
7886-7893
1990
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29857
Maurizi
Sequence and structure of Clp ...
Escherichia coli
J. Biol. Chem.
265
12536-12545
1990
-
-
1
-
-
-
-
-
-
-
2
-
-
4
-
-
1
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
1
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
29859
Gottesman
Conservation of the regulatory ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
87
3513-3517
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29868
Maurizi
Clp P represents a unique fami ...
Escherichia coli
J. Biol. Chem.
265
12546-12552
1990
-
-
1
-
-
-
1
-
2
-
-
1
-
6
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29864
Woo
Protease Ti from Escherichia c ...
Escherichia coli, Escherichia coli RGC125 (lon-)
J. Biol. Chem.
264
2088-2091
1989
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3
1
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4
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6
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1
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1
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3
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1
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6
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29860
Hwang
Protease Ti, a new ATP-depende ...
Escherichia coli, Escherichia coli RGC125 (lon-)
J. Biol. Chem.
263
8727-8734
1988
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-
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1
1
1
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3
3
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4
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1
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1
4
2
2
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1
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1
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2
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2
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1
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1
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1
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3
3
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1
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1
4
2
2
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1
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1
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29862
Katayama
The two-component, ATP-depende ...
Escherichia coli
J. Biol. Chem.
263
15226-15236
1988
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1
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1
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4
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1
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1
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1
2
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1
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1
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1
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1
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1
2
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29861
Katayama-Fujimura
A multiple-component, ATP-depe ...
Escherichia coli
J. Biol. Chem.
262
4477-4485
1987
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2
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1
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1
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1
1
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2
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1
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1
1
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