Literature summary for 3.4.21.91 extracted from

Bessaud, M.; Pastorino, B.A.; Peyrefitte, C.N.; Rolland, D.; Grandadam, M.; Tolou, H.J.
Functional characterization of the NS2B/NS3 protease complex from seven viruses belonging to different groups inside the genus Flavivirus (2006), Virus Res., 120, 79-90.

Search for more literature for 3.4.21.91

Cloned(Commentary)

Cloned (Comment)	Organism
deltaNS2B-NS3pro and deltaNS2B-NS3proHDA expressed as a hexahistidine-tagged protein in Escherichia coli BL21-CodonPlus(DE3)-RIL cells	Yellow fever virus
deltaNS2B-NS3pro and deltaNS2B-NS3proHDA expressed as a hexahistidine-tagged protein in Escherichia coli BL21-CodonPlus(DE3)-RIL cells	dengue virus type I
deltaNS2B-NS3pro and deltaNS2B-NS3proHDA expressed as a hexahistidine-tagged protein in Escherichia coli BL21-CodonPlus(DE3)-RIL cells	Langat virus
deltaNS2B-NS3pro and deltaNS2B-NS3proHDA expressed as a hexahistidine-tagged protein in Escherichia coli BL21-CodonPlus(DE3)-RIL cells	Zika virus
deltaNS2B-NS3pro and deltaNS2B-NS3proHDA expressed as a hexahistidine-tagged protein in Escherichia coli BL21-CodonPlus(DE3)-RIL cells	Saint Louis encephalitis virus
deltaNS2B-NS3pro and deltaNS2B-NS3proHDA expressed as a hexahistidine-tagged protein in Escherichia coli BL21-CodonPlus(DE3)-RIL cells	Bussuquara virus
deltaNS2B-NS3pro and deltaNS2B-NS3proHDA expressed as a hexahistidine-tagged protein in Escherichia coli BL21-CodonPlus(DE3)-RIL cells	Dengue virus type 3

Inhibitors

Inhibitors	Comment	Organism
additional information	N-alpha-benzoyl-L-arginine-p-nitroanilide causes precipitation of the recombinant protease and is therefore not appropiate for enzymatic analysis	Bussuquara virus
additional information	N-alpha-benzoyl-L-arginine-p-nitroanilide causes precipitation of the recombinant protease and is therefore not appropiate for enzymatic analysis	Dengue virus type 3
additional information	N-alpha-benzoyl-L-arginine-p-nitroanilide causes precipitation of the recombinant protease and is therefore not appropiate for enzymatic analysis	dengue virus type I
additional information	N-alpha-benzoyl-L-arginine-p-nitroanilide causes precipitation of the recombinant protease and is therefore not appropiate for enzymatic analysis	Langat virus
additional information	N-alpha-benzoyl-L-arginine-p-nitroanilide causes precipitation of the recombinant protease and is therefore not appropiate for enzymatic analysis	Saint Louis encephalitis virus
additional information	N-alpha-benzoyl-L-arginine-p-nitroanilide causes precipitation of the recombinant protease and is therefore not appropiate for enzymatic analysis	Yellow fever virus
additional information	N-alpha-benzoyl-L-arginine-p-nitroanilide causes precipitation of the recombinant protease and is therefore not appropiate for enzymatic analysis	Zika virus
NaCl	decreases activity by more than 40% at 50 mM and more than 70% at 250 mM	Bussuquara virus
NaCl	decreases activity by more than 40% at 50 mM and more than 70% at 250 mM	Dengue virus type 3
NaCl	decreases activity by more than 40% at 50 mM and more than 70% at 250 mM	dengue virus type I
NaCl	decreases activity less than 25% at 250 mM	Langat virus
NaCl	decreases activity by more than 40% at 50 mM and more than 70% at 250 mM	Saint Louis encephalitis virus
NaCl	-	Yellow fever virus
NaCl	decreases activity by more than 40% at 50 mM and more than 70% at 250 mM	Zika virus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.181	-	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	-	Dengue virus type 3
0.324	-	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	-	Langat virus
0.415	-	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	-	Yellow fever virus
0.417	-	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	-	Zika virus
0.465	-	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	-	Saint Louis encephalitis virus
0.505	-	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	-	dengue virus type I
0.53	-	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	-	Yellow fever virus
0.563	-	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	-	Saint Louis encephalitis virus
0.65	-	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	-	Zika virus
1.249	-	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	-	Bussuquara virus
2.18	-	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	-	Bussuquara virus
2.7	-	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	-	Dengue virus type 3
3.74	-	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	-	dengue virus type I

Organism

Organism	UniProt	Comment	Textmining
Bussuquara virus	-	-	-
Dengue virus type 3	Q6YMS4	-	-
Dengue virus type 3 Sri Lanka/1266/2000	Q6YMS4	-	-
dengue virus type I	Q91NH1	polyprotein	-
dengue virus type I D1/H/IMTSSA/98/606	Q91NH1	polyprotein	-
Langat virus	P29837	strain TP-21	-
Langat virus TP21	P29837	strain TP-21	-
Saint Louis encephalitis virus	-	-	-
Saint Louis encephalitis virus MSI-7	-	-	-
Yellow fever virus	-	-	-
Yellow fever virus Asibi	-	-	-
Zika virus	-	-	-
Zika virus Ar-D-41644	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
on Ni-NTA column	Yellow fever virus
on Ni-NTA column	dengue virus type I
on Ni-NTA column	Langat virus
on Ni-NTA column	Zika virus
on Ni-NTA column	Saint Louis encephalitis virus
on Ni-NTA column	Bussuquara virus
on Ni-NTA column	Dengue virus type 3

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O	-	Yellow fever virus	N-alpha-benzoyl-L-arginine + p-nitroaniline	-	?
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O	-	dengue virus type I	N-alpha-benzoyl-L-arginine + p-nitroaniline	-	?
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O	-	Langat virus	N-alpha-benzoyl-L-arginine + p-nitroaniline	-	?
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O	-	Zika virus	N-alpha-benzoyl-L-arginine + p-nitroaniline	-	?
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O	-	Saint Louis encephalitis virus	N-alpha-benzoyl-L-arginine + p-nitroaniline	-	?
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O	-	Bussuquara virus	N-alpha-benzoyl-L-arginine + p-nitroaniline	-	?
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O	-	Dengue virus type 3	N-alpha-benzoyl-L-arginine + p-nitroaniline	-	?
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O	-	Yellow fever virus Asibi	N-alpha-benzoyl-L-arginine + p-nitroaniline	-	?
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O	-	Dengue virus type 3 Sri Lanka/1266/2000	N-alpha-benzoyl-L-arginine + p-nitroaniline	-	?
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O	-	dengue virus type I D1/H/IMTSSA/98/606	N-alpha-benzoyl-L-arginine + p-nitroaniline	-	?
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O	-	Langat virus TP21	N-alpha-benzoyl-L-arginine + p-nitroaniline	-	?
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O	-	Zika virus Ar-D-41644	N-alpha-benzoyl-L-arginine + p-nitroaniline	-	?
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O	-	Saint Louis encephalitis virus MSI-7	N-alpha-benzoyl-L-arginine + p-nitroaniline	-	?
t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Yellow fever virus	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	dengue virus type I	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Langat virus	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Zika virus	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Saint Louis encephalitis virus	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Bussuquara virus	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Dengue virus type 3	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Yellow fever virus Asibi	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Dengue virus type 3 Sri Lanka/1266/2000	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	dengue virus type I D1/H/IMTSSA/98/606	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Langat virus TP21	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Zika virus Ar-D-41644	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Saint Louis encephalitis virus MSI-7	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Yellow fever virus	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	dengue virus type I	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Langat virus	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Zika virus	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Saint Louis encephalitis virus	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Bussuquara virus	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Dengue virus type 3	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Yellow fever virus Asibi	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Dengue virus type 3 Sri Lanka/1266/2000	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	dengue virus type I D1/H/IMTSSA/98/606	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Langat virus TP21	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Zika virus Ar-D-41644	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin	-	?
t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Saint Louis encephalitis virus MSI-7	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin	-	?

Synonyms

Synonyms	Comment	Organism
NS2B/NS3 protease complex	-	Yellow fever virus
NS2B/NS3 protease complex	-	dengue virus type I
NS2B/NS3 protease complex	-	Langat virus
NS2B/NS3 protease complex	-	Zika virus
NS2B/NS3 protease complex	-	Saint Louis encephalitis virus
NS2B/NS3 protease complex	-	Bussuquara virus
NS2B/NS3 protease complex	-	Dengue virus type 3

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	-	Yellow fever virus
22	-	-	Langat virus
22	-	-	Bussuquara virus
22	-	-	Dengue virus type 3
28	-	-	Zika virus
37	-	-	dengue virus type I
37	-	-	Saint Louis encephalitis virus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
22	37	-	dengue virus type I

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.006	-	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	-	Yellow fever virus
0.007	-	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	-	Langat virus
0.007	-	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	-	Dengue virus type 3
0.013	-	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	-	Zika virus
0.039	-	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	-	Bussuquara virus
0.044	-	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	-	Yellow fever virus
0.073	-	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	-	Bussuquara virus
0.075	-	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	-	Saint Louis encephalitis virus
0.083	-	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	-	Saint Louis encephalitis virus
0.088	-	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	-	Zika virus
0.101	-	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	-	dengue virus type I
0.153	-	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	-	dengue virus type I
0.163	-	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	-	Dengue virus type 3

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Yellow fever virus
9	-	-	dengue virus type I
9	-	-	Langat virus
9	-	-	Zika virus
9	-	-	Saint Louis encephalitis virus
9	-	-	Bussuquara virus
9	-	-	Dengue virus type 3

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	10.5	-	Saint Louis encephalitis virus
7	10.5	-	Dengue virus type 3
7	10.5	at pH 9.0 the enzyme is 5times more active than at pH 7.0, and more than 3times more active than at pH 10.5	Yellow fever virus
7	10.5	at pH 9.0 the enzyme is 5times more active than at pH 7.0, and more than 3times more active than at pH 10.5	dengue virus type I
7	10.5	at pH 9.0 the enzyme is 5times more active than at pH 7.0, and more than 3times more active than at pH 10.5	Zika virus
7	10.5	at pH 9.0 the enzyme is 5times more active than at pH 7.0, and more than 3times more active than at pH 10.5	Bussuquara virus
7	10.5	is less sensitive to pH variations compared to other flaviviruses, with a reduction in the enzymatic activity lower than 50% at pH 7.0 and 10.5 compared to the maximum activity at pH 9.0	Langat virus