Application | Comment | Organism |
---|---|---|
analysis | NS2B-NS3pro K48A mutant construct is well suited for follow-up purification and structural and drug design studies | West Nile virus |
Cloned (Comment) | Organism |
---|---|
NS2B-NS3pro wild-type and K48A constructs cloned into pET101 vector and expressed in Escherichia coli BL21 (DE3) Codon Plus cells | West Nile virus |
Protein Variants | Comment | Organism |
---|---|---|
K48A | is a single-chain protein and fully resistant to autolysis at the linker region, exhibits high stability and functional activity, cleaves pyroglutamic acid-Arg-Thr-Lys-Arg-7-amino-4-methylcoumarin | West Nile virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aprotinin | completely inhibits autolysis of the NS2B-NS3pro construct | West Nile virus | |
decanoyl-Arg-Val-Lys-Arg-chloromethylketone | completely inhibits autolysis of the NS2B-NS3pro construct | West Nile virus | |
nona-D-Arg-amide | completely inhibits autolysis of the NS2B-NS3pro construct | West Nile virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
West Nile virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
NS2B-NS3pro wild-type and K48A constructs purified by Co2+ affinity chromatography and gel filtration, to homogeneity | West Nile virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | during purification, incomplete autolytic conversion of the 36 kDa NS2B-NS3pro construct into the individual 30 kDa NS3pro and the NS2B sequence (5-6 kDa). Undergoes self-cleavage during expression and purification at the K48G-G site of the glycine linker | West Nile virus | ? | - |
? | |
pyroglutamic acid-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O | - |
West Nile virus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NS2BNS3(pro)tease | - |
West Nile virus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000006 | - |
nona-D-Arg-amide | - |
West Nile virus | |
0.000025 | - |
Aprotinin | - |
West Nile virus | |
0.0004 | - |
decanoyl-Arg-Val-Lys-Arg-chloromethylketone | - |
West Nile virus |