Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | formation of an active protease does not require neither cleavage of NS2B from NS3 nor a free NS3 N-terminus | West Nile virus |
Cloned (Comment) | Organism |
---|---|
plasmid pDV2-NS2B40-G4SG4-ASR-NS3FL expressed in Escherichia coli strain Rosetta2 | Dengue virus type 2 |
plasmid pDV2-NS2B40-G4SG4-ASR-NS3FL expressed in Escherichia coli strain Rosetta2 | Dengue virus type 4 |
plasmid pJEVNS2B40-G4SG4-ASR-NS3FL expressed in Escherichia coli strain Rosetta2 | Japanese encephalitis virus |
plasmid pWNV-NS2B79-NS3FL expressed in Escherichia coli strain Rosetta2 | West Nile virus |
plasmid pYFV-NS2B40-G4SG4-HM-NS3FL expressed in Escherichia coli strain Rosetta2 | Yellow fever virus |
Protein Variants | Comment | Organism |
---|---|---|
G460L | no autolytic cleavage, but is an efficient enzyme against the artificial substrate CFP-LQYTKRGGVLWD-YFP | West Nile virus |
S135A | no autolytic cleavage, is enzymatically inactive | West Nile virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | product inhibition of the protease by the cleaved C-terminus of NS2B. The reaction product Lys-Arg-COO- remains in the P2-P1 site after cleavage and effectively blocks the active site for both cis and trans substrates | West Nile virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dengue virus type 2 | - |
- |
- |
Dengue virus type 4 | - |
- |
- |
Japanese encephalitis virus | - |
- |
- |
West Nile virus | - |
- |
- |
West Nile virus NY99 | - |
- |
- |
Yellow fever virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by anion-exchange chromatography | Yellow fever virus |
by anion-exchange chromatography | West Nile virus |
by anion-exchange chromatography | Dengue virus type 2 |
by anion-exchange chromatography | Dengue virus type 4 |
Storage Stability | Organism |
---|---|
-20°C, 15% glycerol | West Nile virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CFP-LQYTKRGGVLWD-YFP + H2O | the artificial substrate contains the natural sequence of the NS2B/NS3 junction and is cleaved efficiently | West Nile virus | CFP-LQYTKR + GGVLWD-YFP | - |
? | |
CFP-LQYTKRGGVLWD-YFP + H2O | the artificial substrate contains the natural sequence of the NS2B/NS3 junction and is cleaved efficiently | West Nile virus NY99 | CFP-LQYTKR + GGVLWD-YFP | - |
? | |
CFP-SAAQRRGRIGRN-YFP + H2O | cleavage is barely detectable | West Nile virus | CFP-SAAQRR + GRIGRN-YFP | - |
? | |
CFP-SAAQRRGRIGRN-YFP + H2O | cleavage is barely detectable | West Nile virus NY99 | CFP-SAAQRR + GRIGRN-YFP | - |
? | |
LQYTKRGGVLWD + H2O | the artificial substrate contains the natural sequence of the NS2B/NS3 junction and is cleaved efficiently | West Nile virus | LQYTKR + GGVLWD | - |
? | |
LQYTKRGGVLWD + H2O | the artificial substrate contains the natural sequence of the NS2B/NS3 junction and is cleaved efficiently | West Nile virus NY99 | LQYTKR + GGVLWD | - |
? | |
additional information | the recombinant NS2B-NS3 protease is subject to autolytic cleavage | Yellow fever virus | ? | - |
? | |
additional information | the recombinant NS2B-NS3 protease is subject to autolytic cleavage | Japanese encephalitis virus | ? | - |
? | |
additional information | the recombinant NS2B-NS3 protease is subject to autolytic cleavage | Dengue virus type 2 | ? | - |
? | |
additional information | the recombinant NS2B-NS3 protease is subject to autolytic cleavage | Dengue virus type 4 | ? | - |
? | |
additional information | the recombinant NS2B-NS3 protease is subject to autolytic cleavage. It catalyzes two autolytic cleavages. The NS2B/NS3 junction is cleaved before protein purification. A second site at Arg4592Gly460 within the C-terminal helicase region of NS3 is cleaved more slowly. Intramolecular cleavage of both the N-terminal site and the internal helicase site of recombinant NS2B-NS3. The artificial substrates CFP-LQYTHMGGVLWD-YFP and CFP-GGGGHMGGVLWD-YFP are not cleaved | West Nile virus | ? | - |
? | |
additional information | the recombinant NS2B-NS3 protease is subject to autolytic cleavage. It catalyzes two autolytic cleavages. The NS2B/NS3 junction is cleaved before protein purification. A second site at Arg4592Gly460 within the C-terminal helicase region of NS3 is cleaved more slowly. Intramolecular cleavage of both the N-terminal site and the internal helicase site of recombinant NS2B-NS3. The artificial substrates CFP-LQYTHMGGVLWD-YFP and CFP-GGGGHMGGVLWD-YFP are not cleaved | West Nile virus NY99 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NS2B-NS3 protease | - |
Yellow fever virus |
NS2B-NS3 protease | - |
West Nile virus |
NS2B-NS3 protease | - |
Japanese encephalitis virus |
NS2B-NS3 protease | - |
Dengue virus type 2 |
NS2B-NS3 protease | - |
Dengue virus type 4 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NS2B protein | - |
Yellow fever virus | |
NS2B protein | - |
West Nile virus | |
NS2B protein | - |
Japanese encephalitis virus | |
NS2B protein | - |
Dengue virus type 2 | |
NS2B protein | - |
Dengue virus type 4 |