Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.91 extracted from

  • Niyomrattanakit, P.; Winoyanuwattikun, P.; Chanprapaph, S.; Angsuthanasombat, C.; Panyim, S.; Katzenmeier, G.
    Identification of residues in the dengue virus type 2 NS2B cofactor that are critical for NS3 protease activation (2004), J. Virol., 78, 13708-13716.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.91

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.146
-
 Gly-Arg-Arg-4-methylcoumarin 7-amide pH 9.0, 37°C, wild-type NS3 protease with wild-tyüe NS2B cofactor Dengue virus type 2

Organism

Organism UniProt Comment Textmining
Dengue virus type 2
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Dengue virus type 2 Gly-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
NS3 protease
-
 Dengue virus type 2

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 Gly-Arg-Arg-4-methylcoumarin 7-amide pH 9.0, 37°C, wild-type NS3 protease with wild-tyüe NS2B cofactor Dengue virus type 2

Cofactor

Cofactor Comment Organism Structure
additional information characterization of structural determinants necessary for activation of the enzyme by the NS2B cofactor. Alanine substitutions at residues Trp62, Leu75, and Ile79 in the NS2B cofactor result in marked effects on autoprocessing at the NS2B/NS3 site. The activity of the mutant NS3 protease with the synthetic peptide substrate Gly-Arg-Arg-4-methylcoumarin 7-amide is mainly affected by significantly reduced kcat values Dengue virus type 2