BRENDA - Enzyme Database
show all sequences of 3.4.21.90

Role of sindbis virus capsid protein region II in nucleocapsid core assembly and encapsidation of genomic RNA

Warrier, R.; Linger, B.R.; Golden, B.L.; Kuhn, R.J.; J. Virol. 82, 4461-4470 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
peptide comprising amino acid residues 81 to 112 of the capsid protein expressed as an Smt3 fusion protein from a modified pET28b vector
Sindbis virus
Engineering
Amino acid exchange
Commentary
Organism
CDELTA(101-105)
core accumulation, no encapsidation specificity
Sindbis virus
CDELTA(101-110)
no core accumulation, no encapsidation specificity
Sindbis virus
CDELTA(106-110)
no core accumulation, no encapsidation specificity
Sindbis virus
CDELTA(107-113)
no core accumulation, no encapsidation specificity
Sindbis virus
CDELTA(91-100)
no core accumulation, no encapsidation specificity
Sindbis virus
CDELTA(96-100)
core accumulation, no encapsidation specificity
Sindbis virus
CDELTA(97-106)
core accumulation, no encapsidation specificity
Sindbis virus
K102A
stronger binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
K109A
stronger binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
K83A
binding affinity for encapsidation signal RNA comparable to the wild-type
Sindbis virus
K86A
stronger binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
K92A
weaker binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
K97A
binding affinity for encapsidation signal RNA comparable to the wild-type
Sindbis virus
K97A/K99A
10fold reduced binding affinity compared to that of the wild type
Sindbis virus
K99A
weaker binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
K99E
accumulates wild-type levels of nucleocapsid cores while still encapsidating approximately 65% viral RNA
Sindbis virus
K99E/R105A
accumulates wild-type levels of nucleocapsid cores while still encapsidating approximately 65% viral RNA
Sindbis virus
K99L
weaker binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
K99M
stronger binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
K99R
stronger binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
L108A
stronger binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
L108D
no core accumulation, no encapsidation specificity
Sindbis virus
L110A
stronger binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
L110N
no core accumulation, no encapsidation specificity
Sindbis virus
P100A
weaker binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
Q104A
binding affinity for encapsidation signal RNA comparable to the wild-type
Sindbis virus
Q88A
weaker binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
Q94A
binding affinity for encapsidation signal RNA comparable to the wild-type
Sindbis virus
R103A
core accumulation, no encapsidation specificity
Sindbis virus
R103A/R105A
100fold reduced binding affinity compared to that of the wild type
Sindbis virus
R105A
core accumulation, no encapsidation specificity
Sindbis virus
R105E
weaker binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
R105K
stronger binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
R105L
weaker binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
R105M
weaker binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sindbis virus
-
-
-
Purification (Commentary)
Commentary
Organism
capsid protein(81-112) mutant, on Ni-agarose affinity column
Sindbis virus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the region that is responsible for nucleocapsid core accumulation has considerable overlap with the region that controls encapsidation specificity. Amino acids 96 to 113 are responsible for the specific encapsidation of genomic RNA. Amino acids 99 and 105 may interact directly with the encapsidation signal. Amino acids 106 to 113 are responsible for nucleocapsid core accumulation
699899
Sindbis virus
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
peptide comprising amino acid residues 81 to 112 of the capsid protein expressed as an Smt3 fusion protein from a modified pET28b vector
Sindbis virus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
CDELTA(101-105)
core accumulation, no encapsidation specificity
Sindbis virus
CDELTA(101-110)
no core accumulation, no encapsidation specificity
Sindbis virus
CDELTA(106-110)
no core accumulation, no encapsidation specificity
Sindbis virus
CDELTA(107-113)
no core accumulation, no encapsidation specificity
Sindbis virus
CDELTA(91-100)
no core accumulation, no encapsidation specificity
Sindbis virus
CDELTA(96-100)
core accumulation, no encapsidation specificity
Sindbis virus
CDELTA(97-106)
core accumulation, no encapsidation specificity
Sindbis virus
K102A
stronger binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
K109A
stronger binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
K83A
binding affinity for encapsidation signal RNA comparable to the wild-type
Sindbis virus
K86A
stronger binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
K92A
weaker binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
K97A
binding affinity for encapsidation signal RNA comparable to the wild-type
Sindbis virus
K97A/K99A
10fold reduced binding affinity compared to that of the wild type
Sindbis virus
K99A
weaker binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
K99E
accumulates wild-type levels of nucleocapsid cores while still encapsidating approximately 65% viral RNA
Sindbis virus
K99E/R105A
accumulates wild-type levels of nucleocapsid cores while still encapsidating approximately 65% viral RNA
Sindbis virus
K99L
weaker binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
K99M
stronger binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
K99R
stronger binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
L108A
stronger binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
L108D
no core accumulation, no encapsidation specificity
Sindbis virus
L110A
stronger binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
L110N
no core accumulation, no encapsidation specificity
Sindbis virus
P100A
weaker binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
Q104A
binding affinity for encapsidation signal RNA comparable to the wild-type
Sindbis virus
Q88A
weaker binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
Q94A
binding affinity for encapsidation signal RNA comparable to the wild-type
Sindbis virus
R103A
core accumulation, no encapsidation specificity
Sindbis virus
R103A/R105A
100fold reduced binding affinity compared to that of the wild type
Sindbis virus
R105A
core accumulation, no encapsidation specificity
Sindbis virus
R105E
weaker binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
R105K
stronger binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
R105L
weaker binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
R105M
weaker binding affinity for encapsidation signal RNA than the wild-type
Sindbis virus
Purification (Commentary) (protein specific)
Commentary
Organism
capsid protein(81-112) mutant, on Ni-agarose affinity column
Sindbis virus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the region that is responsible for nucleocapsid core accumulation has considerable overlap with the region that controls encapsidation specificity. Amino acids 96 to 113 are responsible for the specific encapsidation of genomic RNA. Amino acids 99 and 105 may interact directly with the encapsidation signal. Amino acids 106 to 113 are responsible for nucleocapsid core accumulation
699899
Sindbis virus
?
-
-
-
-
Other publictions for EC 3.4.21.90
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
695938
Wengler
The regulation of disassembly ...
Chikungunya virus, Ross River virus, Semliki forest virus, Sindbis virus
Arch. Virol.
154
381-390
2009
2
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11
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699899
Warrier
Role of sindbis virus capsid p ...
Sindbis virus
J. Virol.
82
4461-4470
2008
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1
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35
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3
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1
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1
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1
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35
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1
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1
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29850
Tong
Refined structure of Sindbis v ...
Sindbis virus
J. Mol. Biol.
230
228-247
1993
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1
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1
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1
1
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2
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2
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1
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1
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1
1
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2
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29851
Kräusslich
Viral proteinases ...
Sindbis virus
Annu. Rev. Biochem.
57
701-754
1988
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