BRENDA - Enzyme Database show
show all sequences of 3.4.21.9

Production of recombinant bovine enterokinase catalytic subunit in Escherichia coli using novel secretory fusion partner DsbA

Collins-Racie, L.A.; McColgan, J.M.; Grant, K.L.; DiBlasio-Smith, A.; McCoy, J.M.; LaVallie, E.R.; Biotechnology 13, 982-987 (1995)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of catalytic light subunit in Escherichia coli
Bos taurus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Trypsinogen + H2O
Bos taurus
the enzyme plays a key role in initiating the proteolytic digestion cascade in duodenum by converting trypsinogen to trypsin
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Trypsinogen + H2O
the enzyme plays a key role in initiating the proteolytic digestion cascade in duodenum by converting trypsinogen to trypsin
95585
Bos taurus
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of catalytic light subunit in Escherichia coli
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Trypsinogen + H2O
Bos taurus
the enzyme plays a key role in initiating the proteolytic digestion cascade in duodenum by converting trypsinogen to trypsin
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Trypsinogen + H2O
the enzyme plays a key role in initiating the proteolytic digestion cascade in duodenum by converting trypsinogen to trypsin
95585
Bos taurus
?
-
-
-
-
Other publictions for EC 3.4.21.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732551
Jirickova
-
Electrochemical determination ...
Homo sapiens
Monatsh. Chem.
146
755-759
2015
-
-
1
-
-
-
-
2
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
2
2
732803
Azhar
Production and purification of ...
Bos taurus
Prep. Biochem. Biotechnol.
45
268-278
2015
-
-
1
-
-
-
-
1
-
-
-
1
-
4
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
732855
Xu
Structure basis for the unique ...
Oryzias latipes
Protein Cell
5
178-181
2014
-
-
1
1
4
-
-
13
-
-
-
1
-
2
-
-
1
-
1
-
-
-
4
-
-
-
-
15
-
-
-
-
-
-
-
-
-
1
-
1
4
-
-
-
-
13
-
-
-
1
-
-
-
1
1
-
-
-
4
-
-
-
-
15
-
-
-
-
-
-
-
-
14
14
731383
Gasparian
Heterogeneous catalysis on the ...
Homo sapiens
Biochimie
95
2076-2081
2013
-
-
1
-
-
-
1
2
-
-
1
1
-
4
-
-
-
-
-
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2
1
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2
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1
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1
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2
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1
1
-
-
-
-
-
-
-
-
2
1
-
-
-
2
-
-
-
-
-
-
-
-
2
2
732883
Smith
Human enteropeptidase light ch ...
Homo sapiens
Protein Sci.
22
577-585
2013
-
-
1
-
2
-
1
9
-
1
1
1
-
3
-
-
1
-
-
-
-
-
4
1
-
-
-
9
-
-
-
-
3
-
-
-
-
1
-
-
2
-
-
1
3
9
-
1
1
1
-
-
-
1
-
-
-
-
4
1
-
-
-
9
-
-
-
-
-
-
-
-
9
9
732195
Ostapchenko
Dissecting structural basis of ...
Bos taurus, Homo sapiens
J. Biomol. Struct. Dyn.
30
62-73
2012
-
-
-
-
-
-
-
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2
-
5
-
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4
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2
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-
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-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
732698
Prohaska
Interaction of protein C inhib ...
Homo sapiens
PLoS ONE
7
e39262
2012
-
-
-
-
-
-
1
-
1
-
1
2
-
2
-
-
-
-
-
6
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
2
-
-
-
-
-
6
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
732888
Simeonov
Crystal structure of a superch ...
Homo sapiens
Proteins
80
1907-1910
2012
-
-
1
1
1
-
-
-
1
-
-
1
-
3
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
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-
-
1
-
1
1
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-
-
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1
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1
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1
1
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-
-
1
-
-
-
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-
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-
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-
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-
-
717409
Chun
Design and efficient productio ...
Bos taurus
Biotechnol. Lett.
33
1227-1232
2011
-
1
1
-
7
-
-
-
1
1
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1
-
3
-
-
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-
-
1
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
7
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
6
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
717431
Choi
A fluorogenic method for measu ...
Homo sapiens
BMB Rep.
44
458-461
2011
-
-
-
-
-
-
1
2
-
-
-
-
-
2
-
-
-
-
-
1
-
-
3
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
2
1
-
-
-
-
1
1
-
2
2
717891
Pepeliaev
High level expression of human ...
Homo sapiens
J. Biotechnol.
156
67-75
2011
-
1
1
-
-
-
-
-
-
-
1
1
-
5
-
-
-
-
-
2
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
2
-
-
2
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
718100
Shirokov
Development of hybrid human in ...
Sus scrofa
Mol. Biol.
45
466-471
2011
-
1
-
-
-
-
-
-
-
-
-
1
-
4
-
-
1
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-
1
1
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3
-
1
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-
-
2
-
-
-
-
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-
1
-
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-
-
-
-
-
-
-
-
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-
1
-
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-
1
-
1
1
-
3
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
718352
Simeonov
Surface supercharged human ent ...
Homo sapiens
Protein Eng. Des. Sel.
24
261-268
2011
-
1
1
-
3
-
-
-
-
-
-
-
-
2
-
-
1
-
1
1
-
-
-
-
1
-
3
-
1
-
-
-
-
-
-
-
1
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
718359
Gasparian
Strategy for improvement of en ...
Bos taurus, Homo sapiens
Protein Expr. Purif.
79
191-196
2011
-
2
-
-
2
-
-
8
2
2
-
-
-
4
-
-
-
-
-
2
-
-
10
2
4
-
-
8
2
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
8
2
2
-
-
-
-
-
-
-
2
-
-
10
2
4
-
-
8
2
-
-
-
-
2
2
-
8
8
717231
Makarova
Effect of enteropeptidase on s ...
Bos taurus, Homo sapiens
Biochemistry (Moscow)
75
1153-1159
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
707812
Kubitzki
Application of immobilized bov ...
Bos taurus
Biotechnol. J.
4
1610-1618
2009
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
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-
-
-
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-
-
-
-
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-
-
1
1
-
-
-
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-
1
-
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683266
Kubitzki
Immobilisation of bovine enter ...
Bos taurus
Bioprocess Biosyst. Eng.
31
173-182
2008
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
2
-
2
1
-
-
3
1
-
-
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-
-
1
-
-
-
-
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-
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-
-
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-
-
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-
-
-
-
-
1
2
-
2
1
-
-
3
1
-
-
-
-
-
-
-
-
696841
Kim
Substrate specificities of por ...
Bos taurus, Sus scrofa
Biosci. Biotechnol. Biochem.
72
905-908
2008
-
-
-
-
-
-
-
14
-
-
-
-
-
2
-
-
2
-
-
-
-
-
32
-
-
-
-
14
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
2
-
-
-
-
32
-
-
-
-
14
1
-
-
-
-
-
-
-
-
-
701055
Shahravan
Enhancing the specificity of t ...
synthetic construct
Protein Expr. Purif.
59
314-319
2008
2
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
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-
-
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1
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2
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1
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-
-
1
-
-
-
-
-
-
-
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-
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-
-
-
683192
Liew
An SRLLR motif downstream of t ...
Sus scrofa
Biochimie
89
21-29
2007
-
1
-
-
-
-
-
-
-
-
-
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3
-
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10
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1
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-
-
10
-
-
-
-
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-
683279
Gasparian
Overexpression and refolding o ...
Homo sapiens
Biotechnol. Lett.
29
1567-1573
2007
-
1
-
-
-
-
-
-
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3
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1
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1
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-
-
-
1
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-
-
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-
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-
-
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-
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-
683994
Huang
Functional expression and puri ...
Bos taurus
Prep. Biochem. Biotechnol.
37
205-217
2007
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
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1
1
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-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684030
Tan
Purification and refolding opt ...
Bos taurus
Protein Expr. Purif.
56
40-47
2007
-
1
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684036
Mikhailova
The ways of realization of hig ...
Bos taurus, Homo sapiens
Protein Pept. Lett.
14
227-232
2007
-
-
-
-
-
-
-
7
-
-
-
-
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
716743
Ogiwara
Specificity of the medaka ente ...
Oryzias latipes
Proc. Natl. Acad. Sci. USA
104
7021-7026
2007
-
-
1
-
11
1
-
15
-
-
2
-
-
2
-
-
1
-
-
2
-
-
5
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
11
1
-
-
-
15
-
-
2
-
-
-
-
1
-
2
-
-
5
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
683172
Gasparian
Biochemical characterization o ...
Bos taurus, Homo sapiens
Biochemistry (Moscow)
71
113-119
2006
-
-
2
-
-
1
21
15
-
4
-
-
-
6
-
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95575
Maroux
Purification and specificity o ...
Sus scrofa
J. Biol. Chem.
246
5031-5039
1971
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