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Literature summary for 3.4.21.9 extracted from

  • Simeonov, P.; Zahn, M.; Straeter, N.; Zuchner, T.
    Crystal structure of a supercharged variant of the human enteropeptidase light chain (2012), Proteins, 80, 1907-1910.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) codon plus RIL hEPL sc C122S cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 20% (w/v) polyethylene glycol 10000, 0.1 M HEPES, pH 7.5 Homo sapiens

Protein Variants

Protein Variants Comment Organism
N6D/G21D/G22D/N142D/K210E/C112S supercharged variant with increased solubility more than 100fold, used for crystallization Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trypsinogen + H2O Homo sapiens
-
trypsin + ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P98073
-
-

Purification (Commentary)

Purification (Comment) Organism
STI-agarose column chromatography and Superdex 200 gel filtration Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
fast dilution method in 0.7 M arginine, 1 mM EDTA, 3 mM reduced glutathione, and 3 mM GSSG at pH 8.6 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trypsinogen + H2O
-
Homo sapiens trypsin + ?
-
?