Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) codon plus RIL hEPL sc C122S cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 20% (w/v) polyethylene glycol 10000, 0.1 M HEPES, pH 7.5 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
N6D/G21D/G22D/N142D/K210E/C112S | supercharged variant with increased solubility more than 100fold, used for crystallization | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trypsinogen + H2O | Homo sapiens | - |
trypsin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P98073 | - |
- |
Purification (Comment) | Organism |
---|---|
STI-agarose column chromatography and Superdex 200 gel filtration | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
fast dilution method in 0.7 M arginine, 1 mM EDTA, 3 mM reduced glutathione, and 3 mM GSSG at pH 8.6 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trypsinogen + H2O | - |
Homo sapiens | trypsin + ? | - |
? |