Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris X-33 strain | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R96Q | the mutant shows decreased specificities for substrates containing the sequences DDDDK and DDDDR, while basic pancreatic trypsin inhibitor inhibition is increased | Homo sapiens |
Y174R | the mutant shows improved specificities for substrates containing the sequences DDDDK and DDDDR, while basic pancreatic trypsin inhibitor inhibition is significantly decreased | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
basic pancreatic trypsin inhibitor | reversible inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00258 | - |
GD4R-4-nitroanilide | mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
0.00629 | - |
GD4R-4-nitroanilide | wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
0.0179 | - |
GDDDDK-4-nitroanilide | mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
0.018 | - |
GD4R-4-nitroanilide | mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
0.0298 | - |
GDDDDK-4-nitroanilide | wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
0.165 | - |
Z-Lys-SBzl | mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
0.172 | - |
Z-Lys-SBzl | mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
0.19 | - |
Z-Lys-SBzl | wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
0.276 | - |
GDDDDK-4-nitroanilide | mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | CaCl2 enhances enzyme activity at 0.02 mM | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
x * 26000, recombinant catalytic subunit, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trypsinogen + H2O | Homo sapiens | cleavage sequence DDDDK-X | trypsin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P98073 | - |
- |
Purification (Comment) | Organism |
---|---|
soybean trypsin inhibitor-agarose column chromatography, and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GD4R-4-nitroanilide + H2O | - |
Homo sapiens | ? | - |
? | |
GDDDDK-4-nitroanilide + H2O | - |
Homo sapiens | GDDDDK + 4-nitroaniline | - |
? | |
trypsinogen + H2O | cleavage sequence DDDDK-X | Homo sapiens | trypsin + ? | - |
? | |
Z-Lys-SBzl + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000, recombinant catalytic subunit, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
enterokinase | - |
Homo sapiens |
EPL | catalytic subunit | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
42 | - |
GD4R-4-nitroanilide | mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
45 | - |
GD4R-4-nitroanilide | wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
49 | - |
GD4R-4-nitroanilide | mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
122 | - |
GDDDDK-4-nitroanilide | mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
135 | - |
GDDDDK-4-nitroanilide | wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
148 | - |
GDDDDK-4-nitroanilide | mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
319 | - |
Z-Lys-SBzl | mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
336 | - |
Z-Lys-SBzl | wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
354 | - |
Z-Lys-SBzl | mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000461 | - |
basic pancreatic trypsin inhibitor | wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
0.0000632 | - |
basic pancreatic trypsin inhibitor | mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
0.000228 | - |
basic pancreatic trypsin inhibitor | mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
537 | - |
GDDDDK-4-nitroanilide | mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
1770 | - |
Z-Lys-SBzl | wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
1860 | - |
Z-Lys-SBzl | mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
2150 | - |
Z-Lys-SBzl | mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
2330 | - |
GD4R-4-nitroanilide | mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
4530 | - |
GDDDDK-4-nitroanilide | wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
6830 | - |
GDDDDK-4-nitroanilide | mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
7200 | - |
GD4R-4-nitroanilide | wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens | |
18900 | - |
GD4R-4-nitroanilide | mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C | Homo sapiens |