BRENDA - Enzyme Database show
show all sequences of 3.4.21.9

Strategy for improvement of enteropeptidase efficiency in tag removal processes

Gasparian, M.E.; Bychkov, M.L.; Dolgikh, D.A.; Kirpichnikov, M.P.; Protein Expr. Purif. 79, 191-196 (2011)

Data extracted from this reference:

Application
Application
Commentary
Organism
molecular biology
the high degree of specificity exhibited by enteropeptidase makes it a suitable reagent for cleaving recombinant proteins to remove affinity or other tags. However often unwanted cleavages elsewhere in the protein occur during cleavage of fusions when high amount of enzyme is required
Bos taurus
molecular biology
the high degree of specificity exhibited by enteropeptidase makes it a suitable reagent for cleaving recombinant proteins to remove affinity or other tags. However often unwanted cleavages elsewhere in the protein occur during cleavage of fusions when high amount of enzyme is required
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
additional information
improved efficiency of fusion proteins cleavage by enteropeptidase by substitution of the Lys residue by Arg in specific cleavage sequence (Asp)4-Lys
Bos taurus
additional information
improved efficiency of fusion proteins cleavage by enteropeptidase by substitution of the Lys residue by Arg in specific cleavage sequence (Asp)4-Lys
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.034
-
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
pH 8.4, 37C
Homo sapiens
0.076
-
formyl-Ala-Phe-Arg-4-nitroanilide
pH 8.4, 25C
Homo sapiens
0.12
-
thiobenzyl benzyloxy-carbonyl-L-lysinate
pH 8.4, 25C
Bos taurus
0.14
-
thiobenzyl benzyloxy-carbonyl-L-lysinate
pH 8.4, 25C
Homo sapiens
0.141
-
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
pH 8.4, 37C
Bos taurus
0.167
-
formyl-Ala-Phe-Lys-4-nitroanilide
pH 8.4, 25C
Homo sapiens
0.192
-
formyl-Ala-Phe-Arg-4-nitroanilide
pH 8.4, 25C
Bos taurus
0.28
-
formyl-Ala-Phe-Lys-4-nitroanilide
pH 8.4, 25C
Bos taurus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
brush border membrane
-
Bos taurus
31526
-
brush border membrane
-
Homo sapiens
31526
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
required
Bos taurus
Ca2+
required
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
intestine
-
Bos taurus
-
intestine
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
formyl-Ala-Phe-Arg-4-nitroanilide + H2O
a chromogenic peptide substrates
718359
Homo sapiens
?
-
-
-
?
formyl-Ala-Phe-Arg-4-nitroanilide + H2O
a chromogenic peptide substrates
718359
Bos taurus
?
-
-
-
?
formyl-Ala-Phe-Lys-4-nitroanilide + H2O
a chromogenic peptide substrates
718359
Homo sapiens
?
-
-
-
?
formyl-Ala-Phe-Lys-4-nitroanilide + H2O
a chromogenic peptide substrates
718359
Bos taurus
?
-
-
-
?
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O
-
718359
Homo sapiens
Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine
-
-
-
?
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O
-
718359
Bos taurus
Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine
-
-
-
?
additional information
high degree of cleavage specificity is exhibited by enteropeptidase
718359
Homo sapiens
?
-
-
-
-
additional information
high degree of cleavage specificity is exhibited by enteropeptidase
718359
Bos taurus
?
-
-
-
-
thiobenzyl benzyloxy-carbonyl-L-lysinate + H2O
-
718359
Homo sapiens
3-carboxy-4-nitrophenoxide + ?
-
-
-
?
thiobenzyl benzyloxy-carbonyl-L-lysinate + H2O
-
718359
Bos taurus
3-carboxy-4-nitrophenoxide + ?
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterodimer
-
Bos taurus
heterodimer
-
Homo sapiens
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at, substrate thiobenzyl benzyloxy-carbonyl-L-lysinate
Bos taurus
25
-
assay at, substrate thiobenzyl benzyloxy-carbonyl-L-lysinate
Homo sapiens
37
-
assay at, substrate Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
Bos taurus
37
-
assay at, substrate Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
Homo sapiens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
15.4
-
formyl-Ala-Phe-Lys-4-nitroanilide
pH 8.4, 25C
Bos taurus
18.2
-
formyl-Ala-Phe-Lys-4-nitroanilide
pH 8.4, 25C
Homo sapiens
23.5
-
formyl-Ala-Phe-Arg-4-nitroanilide
pH 8.4, 25C
Homo sapiens
26.3
-
formyl-Ala-Phe-Arg-4-nitroanilide
pH 8.4, 25C
Bos taurus
49.3
-
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
pH 8.4, 37C
Bos taurus
118
-
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
pH 8.4, 37C
Homo sapiens
129
-
thiobenzyl benzyloxy-carbonyl-L-lysinate
pH 8.4, 25C
Bos taurus
133
-
thiobenzyl benzyloxy-carbonyl-L-lysinate
pH 8.4, 25C
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
assay at
Bos taurus
8.4
-
assay at
Homo sapiens
Application (protein specific)
Application
Commentary
Organism
molecular biology
the high degree of specificity exhibited by enteropeptidase makes it a suitable reagent for cleaving recombinant proteins to remove affinity or other tags. However often unwanted cleavages elsewhere in the protein occur during cleavage of fusions when high amount of enzyme is required
Bos taurus
molecular biology
the high degree of specificity exhibited by enteropeptidase makes it a suitable reagent for cleaving recombinant proteins to remove affinity or other tags. However often unwanted cleavages elsewhere in the protein occur during cleavage of fusions when high amount of enzyme is required
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
improved efficiency of fusion proteins cleavage by enteropeptidase by substitution of the Lys residue by Arg in specific cleavage sequence (Asp)4-Lys
Bos taurus
additional information
improved efficiency of fusion proteins cleavage by enteropeptidase by substitution of the Lys residue by Arg in specific cleavage sequence (Asp)4-Lys
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.034
-
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
pH 8.4, 37C
Homo sapiens
0.076
-
formyl-Ala-Phe-Arg-4-nitroanilide
pH 8.4, 25C
Homo sapiens
0.12
-
thiobenzyl benzyloxy-carbonyl-L-lysinate
pH 8.4, 25C
Bos taurus
0.14
-
thiobenzyl benzyloxy-carbonyl-L-lysinate
pH 8.4, 25C
Homo sapiens
0.141
-
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
pH 8.4, 37C
Bos taurus
0.167
-
formyl-Ala-Phe-Lys-4-nitroanilide
pH 8.4, 25C
Homo sapiens
0.192
-
formyl-Ala-Phe-Arg-4-nitroanilide
pH 8.4, 25C
Bos taurus
0.28
-
formyl-Ala-Phe-Lys-4-nitroanilide
pH 8.4, 25C
Bos taurus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
brush border membrane
-
Bos taurus
31526
-
brush border membrane
-
Homo sapiens
31526
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
required
Bos taurus
Ca2+
required
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
intestine
-
Bos taurus
-
intestine
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
formyl-Ala-Phe-Arg-4-nitroanilide + H2O
a chromogenic peptide substrates
718359
Homo sapiens
?
-
-
-
?
formyl-Ala-Phe-Arg-4-nitroanilide + H2O
a chromogenic peptide substrates
718359
Bos taurus
?
-
-
-
?
formyl-Ala-Phe-Lys-4-nitroanilide + H2O
a chromogenic peptide substrates
718359
Homo sapiens
?
-
-
-
?
formyl-Ala-Phe-Lys-4-nitroanilide + H2O
a chromogenic peptide substrates
718359
Bos taurus
?
-
-
-
?
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O
-
718359
Homo sapiens
Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine
-
-
-
?
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O
-
718359
Bos taurus
Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine
-
-
-
?
additional information
high degree of cleavage specificity is exhibited by enteropeptidase
718359
Homo sapiens
?
-
-
-
-
additional information
high degree of cleavage specificity is exhibited by enteropeptidase
718359
Bos taurus
?
-
-
-
-
thiobenzyl benzyloxy-carbonyl-L-lysinate + H2O
-
718359
Homo sapiens
3-carboxy-4-nitrophenoxide + ?
-
-
-
?
thiobenzyl benzyloxy-carbonyl-L-lysinate + H2O
-
718359
Bos taurus
3-carboxy-4-nitrophenoxide + ?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterodimer
-
Bos taurus
heterodimer
-
Homo sapiens
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at, substrate thiobenzyl benzyloxy-carbonyl-L-lysinate
Bos taurus
25
-
assay at, substrate thiobenzyl benzyloxy-carbonyl-L-lysinate
Homo sapiens
37
-
assay at, substrate Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
Bos taurus
37
-
assay at, substrate Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
Homo sapiens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
15.4
-
formyl-Ala-Phe-Lys-4-nitroanilide
pH 8.4, 25C
Bos taurus
18.2
-
formyl-Ala-Phe-Lys-4-nitroanilide
pH 8.4, 25C
Homo sapiens
23.5
-
formyl-Ala-Phe-Arg-4-nitroanilide
pH 8.4, 25C
Homo sapiens
26.3
-
formyl-Ala-Phe-Arg-4-nitroanilide
pH 8.4, 25C
Bos taurus
49.3
-
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
pH 8.4, 37C
Bos taurus
118
-
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
pH 8.4, 37C
Homo sapiens
129
-
thiobenzyl benzyloxy-carbonyl-L-lysinate
pH 8.4, 25C
Bos taurus
133
-
thiobenzyl benzyloxy-carbonyl-L-lysinate
pH 8.4, 25C
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
assay at
Bos taurus
8.4
-
assay at
Homo sapiens
General Information
General Information
Commentary
Organism
additional information
improved efficiency of fusion proteins cleavage by enteropeptidase by substitution of the Lys residue by Arg in specific cleavage sequence (Asp)4-Lys. 3-6fold lower amounts of the catalytic subunit of enteropeptidase is required for 95% cleavage of Trx/TRAIL and Trx/FGF-2 fusions with (Asp)4-Arg cleavage sequence in comparison to native sequence (Asp)4-Lys. Reduced amount of non-specifically cleaved peptide fragments are observed during cleavage of (Asp)4-Lys/Arg mutated fusions
Bos taurus
additional information
improved efficiency of fusion proteins cleavage by enteropeptidase by substitution of the Lys residue by Arg in specific cleavage sequence (Asp)4-Lys. 3-6fold lower amounts of the catalytic subunit of enteropeptidase is required for 95% cleavage of Trx/TRAIL and Trx/FGF-2 fusions with (Asp)4-Arg cleavage sequence in comparison to native sequence (Asp)4-Lys. Reduced amount of non-specifically cleaved peptide fragments are observed during cleavage of (Asp)4-Lys/Arg mutated fusions
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
additional information
improved efficiency of fusion proteins cleavage by enteropeptidase by substitution of the Lys residue by Arg in specific cleavage sequence (Asp)4-Lys. 3-6fold lower amounts of the catalytic subunit of enteropeptidase is required for 95% cleavage of Trx/TRAIL and Trx/FGF-2 fusions with (Asp)4-Arg cleavage sequence in comparison to native sequence (Asp)4-Lys. Reduced amount of non-specifically cleaved peptide fragments are observed during cleavage of (Asp)4-Lys/Arg mutated fusions
Bos taurus
additional information
improved efficiency of fusion proteins cleavage by enteropeptidase by substitution of the Lys residue by Arg in specific cleavage sequence (Asp)4-Lys. 3-6fold lower amounts of the catalytic subunit of enteropeptidase is required for 95% cleavage of Trx/TRAIL and Trx/FGF-2 fusions with (Asp)4-Arg cleavage sequence in comparison to native sequence (Asp)4-Lys. Reduced amount of non-specifically cleaved peptide fragments are observed during cleavage of (Asp)4-Lys/Arg mutated fusions
Homo sapiens
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
55
-
formyl-Ala-Phe-Lys-4-nitroanilide
pH 8.4, 25C
Bos taurus
109
-
formyl-Ala-Phe-Lys-4-nitroanilide
pH 8.4, 25C
Homo sapiens
137
-
formyl-Ala-Phe-Arg-4-nitroanilide
pH 8.4, 25C
Bos taurus
309
-
formyl-Ala-Phe-Arg-4-nitroanilide
pH 8.4, 25C
Homo sapiens
347
-
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
pH 8.4, 37C
Bos taurus
950
-
thiobenzyl benzyloxy-carbonyl-L-lysinate
pH 8.4, 25C
Homo sapiens
969
-
thiobenzyl benzyloxy-carbonyl-L-lysinate
pH 8.4, 25C
Bos taurus
3500
-
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
pH 8.4, 37C
Homo sapiens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
55
-
formyl-Ala-Phe-Lys-4-nitroanilide
pH 8.4, 25C
Bos taurus
109
-
formyl-Ala-Phe-Lys-4-nitroanilide
pH 8.4, 25C
Homo sapiens
137
-
formyl-Ala-Phe-Arg-4-nitroanilide
pH 8.4, 25C
Bos taurus
309
-
formyl-Ala-Phe-Arg-4-nitroanilide
pH 8.4, 25C
Homo sapiens
347
-
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
pH 8.4, 37C
Bos taurus
950
-
thiobenzyl benzyloxy-carbonyl-L-lysinate
pH 8.4, 25C
Homo sapiens
969
-
thiobenzyl benzyloxy-carbonyl-L-lysinate
pH 8.4, 25C
Bos taurus
3500
-
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
pH 8.4, 37C
Homo sapiens
Other publictions for EC 3.4.21.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732551
Jirickova
-
Electrochemical determination ...
Homo sapiens
Monatsh. Chem.
146
755-759
2015
-
-
1
-
-
-
-
2
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
2
2
732803
Azhar
Production and purification of ...
Bos taurus
Prep. Biochem. Biotechnol.
45
268-278
2015
-
-
1
-
-
-
-
1
-
-
-
1
-
4
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
732855
Xu
Structure basis for the unique ...
Oryzias latipes
Protein Cell
5
178-181
2014
-
-
1
1
4
-
-
13
-
-
-
1
-
2
-
-
1
-
1
-
-
-
4
-
-
-
-
15
-
-
-
-
-
-
-
-
-
1
-
1
4
-
-
-
-
13
-
-
-
1
-
-
-
1
1
-
-
-
4
-
-
-
-
15
-
-
-
-
-
-
-
-
14
14
731383
Gasparian
Heterogeneous catalysis on the ...
Homo sapiens
Biochimie
95
2076-2081
2013
-
-
1
-
-
-
1
2
-
-
1
1
-
4
-
-
-
-
-
-
-
-
2
1
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
2
-
-
1
1
-
-
-
-
-
-
-
-
2
1
-
-
-
2
-
-
-
-
-
-
-
-
2
2
732883
Smith
Human enteropeptidase light ch ...
Homo sapiens
Protein Sci.
22
577-585
2013
-
-
1
-
2
-
1
9
-
1
1
1
-
3
-
-
1
-
-
-
-
-
4
1
-
-
-
9
-
-
-
-
3
-
-
-
-
1
-
-
2
-
-
1
3
9
-
1
1
1
-
-
-
1
-
-
-
-
4
1
-
-
-
9
-
-
-
-
-
-
-
-
9
9
732195
Ostapchenko
Dissecting structural basis of ...
Bos taurus, Homo sapiens
J. Biomol. Struct. Dyn.
30
62-73
2012
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
732698
Prohaska
Interaction of protein C inhib ...
Homo sapiens
PLoS ONE
7
e39262
2012
-
-
-
-
-
-
1
-
1
-
1
2
-
2
-
-
-
-
-
6
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
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1
2
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2
1
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732888
Simeonov
Crystal structure of a superch ...
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Proteins
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2012
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Chun
Design and efficient productio ...
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717431
Choi
A fluorogenic method for measu ...
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BMB Rep.
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1
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2
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717891
Pepeliaev
High level expression of human ...
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J. Biotechnol.
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2011
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1
1
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1
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2
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2
2
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718100
Shirokov
Development of hybrid human in ...
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Mol. Biol.
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1
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1
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1
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1
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3
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1
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-
2
-
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718352
Simeonov
Surface supercharged human ent ...
Homo sapiens
Protein Eng. Des. Sel.
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261-268
2011
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1
1
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3
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1
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1
1
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3
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1
1
1
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1
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1
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718359
Gasparian
Strategy for improvement of en ...
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Protein Expr. Purif.
79
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2011
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2
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2
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8
2
2
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10
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8
2
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2
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2
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8
2
2
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2
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10
2
4
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8
2
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2
2
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8
8
717231
Makarova
Effect of enteropeptidase on s ...
Bos taurus, Homo sapiens
Biochemistry (Moscow)
75
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2010
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4
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-
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2
2
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707812
Kubitzki
Application of immobilized bov ...
Bos taurus
Biotechnol. J.
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2009
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1
1
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2
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-
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1
1
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-
-
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-
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-
1
-
-
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2
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683266
Kubitzki
Immobilisation of bovine enter ...
Bos taurus
Bioprocess Biosyst. Eng.
31
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2008
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1
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2
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1
2
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2
1
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3
1
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-
-
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-
1
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-
-
-
-
-
-
-
-
-
-
-
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-
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1
2
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2
1
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3
1
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696841
Kim
Substrate specificities of por ...
Bos taurus, Sus scrofa
Biosci. Biotechnol. Biochem.
72
905-908
2008
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14
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32
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14
1
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14
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1
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701055
Shahravan
Enhancing the specificity of t ...
synthetic construct
Protein Expr. Purif.
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2008
2
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1
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1
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2
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1
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1
-
-
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-
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683192
Liew
An SRLLR motif downstream of t ...
Sus scrofa
Biochimie
89
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2007
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1
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10
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1
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10
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683279
Gasparian
Overexpression and refolding o ...
Homo sapiens
Biotechnol. Lett.
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2007
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1
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1
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1
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1
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683994
Huang
Functional expression and puri ...
Bos taurus
Prep. Biochem. Biotechnol.
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205-217
2007
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1
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1
1
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-
1
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-
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-
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-
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684030
Tan
Purification and refolding opt ...
Bos taurus
Protein Expr. Purif.
56
40-47
2007
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1
1
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1
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4
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1
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1
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3
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1
1
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1
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1
-
1
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3
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-
-
-
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684036
Mikhailova
The ways of realization of hig ...
Bos taurus, Homo sapiens
Protein Pept. Lett.
14
227-232
2007
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7
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6
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7
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6
-
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-
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716743
Ogiwara
Specificity of the medaka ente ...
Oryzias latipes
Proc. Natl. Acad. Sci. USA
104
7021-7026
2007
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1
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11
1
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15
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2
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2
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1
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2
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5
1
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1
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1
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1
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-
11
1
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-
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15
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2
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-
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1
-
2
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5
1
-
-
1
-
1
-
-
-
-
-
-
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-
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683172
Gasparian
Biochemical characterization o ...
Bos taurus, Homo sapiens
Biochemistry (Moscow)
71
113-119
2006
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2
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1
21
15
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4
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6
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2
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2
1
16
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1
1
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2
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1
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21
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15
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4
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2
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2
1
16
-
-
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1
1
-
-
-
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-
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684000
Boulware
Protease specificity determina ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
103
7583-7588
2006
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1
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2
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13
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1
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13
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667759
Mikhailova
Effect of calcium ions on ente ...
Homo sapiens
Biochemistry (Moscow)
70
1129-1135
2005
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1
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1
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7
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9
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1
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1
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7
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9
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669370
Nemoda
The tetra-aspartate motif in t ...
Bos taurus, Homo sapiens
J. Biol. Chem.
280
29645-29652
2005
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6
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6
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4
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6
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670773
Liew
Preparation of recombinant thi ...
Sus scrofa
Protein Expr. Purif.
41
332-340
2005
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1
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-
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-
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-
1
-
-
-
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-
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-
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667026
Fang
Expression of recombinant chin ...
Bos taurus
Acta Biochim. Biophys. Sin.
36
513-517
2004
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1
1
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1
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1
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1
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1
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1
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1
1
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-
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-
-
-
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1
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-
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1
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-
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1
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1
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-
-
1
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-
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667758
Mikhailova
Study of secondary specificity ...
Bos taurus
Biochemistry (Moscow)
69
909-917
2004
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1
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9
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1
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1
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1
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9
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12
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1
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-
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9
-
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1
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1
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9
-
-
-
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12
-
-
-
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-
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668257
Huang
Prokaryotic expression of Chin ...
Bos taurus
Chin. Med. Sci.
117
286-290
2004
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1
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1
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1
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1
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1
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1
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1
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669550
Peng
High-level secretory productio ...
Bos taurus
J. Biotechnol.
108
185-192
2004
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1
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1
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1
1
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1
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2
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1
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1
1
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653769
Gasparian
Expression, purification, and ...
Homo sapiens
Protein Expr. Purif.
31
133-139
2003
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1
1
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8
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2
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1
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1
1
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1
1
2
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1
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8
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2
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653747
Yuan
Expression, purification, and ...
Bos taurus
Protein Expr. Purif.
25
300-304
2002
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1
1
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1
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1
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2
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1
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1
1
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1
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1
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95577
Lu
Crystal structure of enteropep ...
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1999
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8
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95584
Matsushima
Purification and further chara ...
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