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Literature summary for 3.4.21.9 extracted from
- Strategy for improvement of enteropeptidase efficiency in tag removal processes (2011), Protein Expr. Purif., 79, 191-196.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | the high degree of specificity exhibited by enteropeptidase makes it a suitable reagent for cleaving recombinant proteins to remove affinity or other tags. However often unwanted cleavages elsewhere in the protein occur during cleavage of fusions when high amount of enzyme is required | Homo sapiens |
| molecular biology | the high degree of specificity exhibited by enteropeptidase makes it a suitable reagent for cleaving recombinant proteins to remove affinity or other tags. However often unwanted cleavages elsewhere in the protein occur during cleavage of fusions when high amount of enzyme is required | Bos taurus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | improved efficiency of fusion proteins cleavage by enteropeptidase by substitution of the Lys residue by Arg in specific cleavage sequence (Asp)4-Lys | Homo sapiens |
| additional information | improved efficiency of fusion proteins cleavage by enteropeptidase by substitution of the Lys residue by Arg in specific cleavage sequence (Asp)4-Lys | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.034 | - |
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide | pH 8.4, 37°C | Homo sapiens |  |
| 0.076 | - |
formyl-Ala-Phe-Arg-4-nitroanilide | pH 8.4, 25°C | Homo sapiens | |
| 0.12 | - |
thiobenzyl benzyloxy-carbonyl-L-lysinate | pH 8.4, 25°C | Bos taurus | |
| 0.14 | - |
thiobenzyl benzyloxy-carbonyl-L-lysinate | pH 8.4, 25°C | Homo sapiens | |
| 0.141 | - |
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide | pH 8.4, 37°C | Bos taurus | |
| 0.167 | - |
formyl-Ala-Phe-Lys-4-nitroanilide | pH 8.4, 25°C | Homo sapiens | |
| 0.192 | - |
formyl-Ala-Phe-Arg-4-nitroanilide | pH 8.4, 25°C | Bos taurus | |
| 0.28 | - |
formyl-Ala-Phe-Lys-4-nitroanilide | pH 8.4, 25°C | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| brush border membrane | - |
Homo sapiens | 31526 | - |
| brush border membrane | - |
Bos taurus | 31526 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Homo sapiens | |
| Ca2+ | required | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| intestine | - |
Homo sapiens | - |
| intestine | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formyl-Ala-Phe-Arg-4-nitroanilide + H2O | a chromogenic peptide substrates | Homo sapiens | ? | - |
? | |
| formyl-Ala-Phe-Arg-4-nitroanilide + H2O | a chromogenic peptide substrates | Bos taurus | ? | - |
? | |
| formyl-Ala-Phe-Lys-4-nitroanilide + H2O | a chromogenic peptide substrates | Homo sapiens | ? | - |
? | |
| formyl-Ala-Phe-Lys-4-nitroanilide + H2O | a chromogenic peptide substrates | Bos taurus | ? | - |
? | |
| Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O | - |
Homo sapiens | Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine | - |
? | |
| Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O | - |
Bos taurus | Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine | - |
? | |
| additional information | high degree of cleavage specificity is exhibited by enteropeptidase | Homo sapiens | ? | - |
? | |
| additional information | high degree of cleavage specificity is exhibited by enteropeptidase | Bos taurus | ? | - |
? | |
| thiobenzyl benzyloxy-carbonyl-L-lysinate + H2O | - |
Homo sapiens | 3-carboxy-4-nitrophenoxide + ? | - |
? | |
| thiobenzyl benzyloxy-carbonyl-L-lysinate + H2O | - |
Bos taurus | 3-carboxy-4-nitrophenoxide + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | - |
Homo sapiens |
| heterodimer | - |
Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BEP | - |
Bos taurus |
| enterokinase | - |
Homo sapiens |
| enterokinase | - |
Bos taurus |
| HEP | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at, substrate thiobenzyl benzyloxy-carbonyl-L-lysinate | Homo sapiens |
| 25 | - |
assay at, substrate thiobenzyl benzyloxy-carbonyl-L-lysinate | Bos taurus |
| 37 | - |
assay at, substrate Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide | Homo sapiens |
| 37 | - |
assay at, substrate Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide | Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15.4 | - |
formyl-Ala-Phe-Lys-4-nitroanilide | pH 8.4, 25°C | Bos taurus | |
| 18.2 | - |
formyl-Ala-Phe-Lys-4-nitroanilide | pH 8.4, 25°C | Homo sapiens | |
| 23.5 | - |
formyl-Ala-Phe-Arg-4-nitroanilide | pH 8.4, 25°C | Homo sapiens | |
| 26.3 | - |
formyl-Ala-Phe-Arg-4-nitroanilide | pH 8.4, 25°C | Bos taurus | |
| 49.3 | - |
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide | pH 8.4, 37°C | Bos taurus | |
| 118 | - |
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide | pH 8.4, 37°C | Homo sapiens | |
| 129 | - |
thiobenzyl benzyloxy-carbonyl-L-lysinate | pH 8.4, 25°C | Bos taurus | |
| 133 | - |
thiobenzyl benzyloxy-carbonyl-L-lysinate | pH 8.4, 25°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
assay at | Homo sapiens |
| 8.4 | - |
assay at | Bos taurus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | improved efficiency of fusion proteins cleavage by enteropeptidase by substitution of the Lys residue by Arg in specific cleavage sequence (Asp)4-Lys. 3-6fold lower amounts of the catalytic subunit of enteropeptidase is required for 95% cleavage of Trx/TRAIL and Trx/FGF-2 fusions with (Asp)4-Arg cleavage sequence in comparison to native sequence (Asp)4-Lys. Reduced amount of non-specifically cleaved peptide fragments are observed during cleavage of (Asp)4-Lys/Arg mutated fusions | Homo sapiens |
| additional information | improved efficiency of fusion proteins cleavage by enteropeptidase by substitution of the Lys residue by Arg in specific cleavage sequence (Asp)4-Lys. 3-6fold lower amounts of the catalytic subunit of enteropeptidase is required for 95% cleavage of Trx/TRAIL and Trx/FGF-2 fusions with (Asp)4-Arg cleavage sequence in comparison to native sequence (Asp)4-Lys. Reduced amount of non-specifically cleaved peptide fragments are observed during cleavage of (Asp)4-Lys/Arg mutated fusions | Bos taurus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 55 | - |
formyl-Ala-Phe-Lys-4-nitroanilide | pH 8.4, 25°C | Bos taurus | |
| 109 | - |
formyl-Ala-Phe-Lys-4-nitroanilide | pH 8.4, 25°C | Homo sapiens | |
| 137 | - |
formyl-Ala-Phe-Arg-4-nitroanilide | pH 8.4, 25°C | Bos taurus | |
| 309 | - |
formyl-Ala-Phe-Arg-4-nitroanilide | pH 8.4, 25°C | Homo sapiens | |
| 347 | - |
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide | pH 8.4, 37°C | Bos taurus | |
| 950 | - |
thiobenzyl benzyloxy-carbonyl-L-lysinate | pH 8.4, 25°C | Homo sapiens | |
| 969 | - |
thiobenzyl benzyloxy-carbonyl-L-lysinate | pH 8.4, 25°C | Bos taurus | |
| 3500 | - |
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide | pH 8.4, 37°C | Homo sapiens | |
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Release 2023.1 (January 2023)
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