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Literature summary for 3.4.21.9 extracted from
- A fluorogenic method for measuring enteropeptidase activity: spectral shift in the emission of GD4K-conjugated 7-amino-4-methylcoumarin (2011), BMB Rep., 44, 458-461.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 50% inhibition at 10 mM | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
GD4K 7-amido-4-methylcoumarin | pH 8.4, 35°C | Homo sapiens | |
| 0.5 | 0.6 | GD4K 2-naphthylamide | pH 8.4, 35°C | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GD4K 2-naphthylamide + H2O | - |
Homo sapiens | ? | - |
? | |
| GD4K 7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | ? | - |
? | |
| additional information | enteropeptidase cleaves the C-terminal end of the substrate recognition sequence Asp-Asp-Asp-Asp-Lys, D4K. Usage of GD4K-conjugated 7-amino-4-methylcoumarin or GD4K-conjugated 2-naphthylamine as a fluorogenic substrates in the assay for enteropeptidase | Homo sapiens | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 24.9 | - |
GD4K 2-naphthylamide | pH 8.4, 35°C | Homo sapiens | |
| 64.8 | - |
GD4K 7-amido-4-methylcoumarin | pH 8.4, 35°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
with substrate GD4K 7-amido-4-methylcoumarin | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | enteropeptidase is a serine protease secreted by the pancreas that converts inactive trypsinogen to active trypsin | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 42 | - |
GD4K 2-naphthylamide | pH 8.4, 35°C | Homo sapiens | |
| 2623 | - |
GD4K 7-amido-4-methylcoumarin | pH 8.4, 35°C | Homo sapiens | |
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Release 2023.1 (January 2023)
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