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Literature summary for 3.4.21.9 extracted from

  • Ogiwara, K.; Takahashi. T.
    Specificity of the medaka enteropeptidase serine protease and its usefulness as a biotechnological tool for fusion-protein cleavage (2007), Proc. Natl. Acad. Sci. USA, 104, 7021-7026.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Oryzias latipes

Protein Variants

Protein Variants Comment Organism
E173A site directed mutagenesis Oryzias latipes
E173K site directed mutagenesis Oryzias latipes
F144A site directed mutagenesis Oryzias latipes
F144S site directed mutagenesis Oryzias latipes
K63A site directed mutagenesis Oryzias latipes
K63E site directed mutagenesis Oryzias latipes
K63R site directed mutagenesis Oryzias latipes
P193A site directed mutagenesis Oryzias latipes
P193E site directed mutagenesis Oryzias latipes
T105A site directed mutagenesis Oryzias latipes
T105E site directed mutagenesis Oryzias latipes

General Stability

General Stability Organism
no loss of activity at -20°C or 4°C for 6 months Oryzias latipes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 Z-Phe-Arg-4-methylcoumaryl-7-amide 37°C, pH 7.4 Oryzias latipes
0.1
-
 Z-Phe-Arg-4-methylcoumaryl-7-amide K63R mutant, 37°C, pH 7.4 Oryzias latipes
0.1
-
 Z-Phe-Arg-4-methylcoumaryl-7-amide T105E mutant, 37°C, pH 7.4 Oryzias latipes
0.2
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide K63R mutant, 37°C, pH 7.4 Oryzias latipes
0.2
-
 Z-Phe-Arg-4-methylcoumaryl-7-amide P193E mutant, 37°C, pH 7.4 Oryzias latipes
0.2
-
 Boc-Glu(OBzl)-Ala-Arg-MCA wild-type enzyme, 37°C, pH 7.4 Oryzias latipes
0.3
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide E173A mutant, 37°C, pH 7.4 Oryzias latipes
0.4
-
 Z-Phe-Arg-4-methylcoumaryl-7-amide E173A mutant, 37°C, pH 7.4 Oryzias latipes
0.4
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide P193E mutant, 37°C, pH 7.4 Oryzias latipes
0.4
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide T105E mutant, 37°C, pH 7.4 Oryzias latipes
0.7
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide 37°C, pH 7.4 Oryzias latipes
1
-
 Pro-Phe-Arg-4-methylcoumaryl-7-amide E173A mutant, 37°C, pH 7.4 Oryzias latipes
1
-
 Boc-Glu(OBzl)-Ala-Arg-MCA E173A mutant, 37°C, pH 7.4 Oryzias latipes
1
-
 Pro-Phe-Arg-4-methylcoumaryl-7-amide P193E mutant, 37°C, pH 7.4 Oryzias latipes
1
-
 Boc-Glu(OBzl)-Ala-Arg-MCA P193E mutant, 37°C, pH 7.4 Oryzias latipes
1.1
-
 Pro-Phe-Arg-4-methylcoumaryl-7-amide K63R mutant, 37°C, pH 7.4 Oryzias latipes
1.2
-
 Boc-Glu(OBzl)-Ala-Arg-MCA K63R mutant, 37°C, pH 7.4 Oryzias latipes
1.3
-
 Pro-Phe-Arg-4-methylcoumaryl-7-amide T105E mutant, 37°C, pH 7.4 Oryzias latipes
1.3
-
 Boc-Glu(OBzl)-Ala-Arg-MCA T105E mutant, 37°C, pH 7.4 Oryzias latipes
10
-
 Pro-Phe-Arg-4-methylcoumaryl-7-amide 37°C, pH 7.4 Oryzias latipes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 Western blot Oryzias latipes
44000
-
 10 * 44000, gel filtration Oryzias latipes

Organism

Organism UniProt Comment Textmining
Oryzias latipes A4UWM5 medaka
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant fusion protein using nickel affinity column chromatography Oryzias latipes

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
 Oryzias latipes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-Glu(OBzl)-Ala-Arg-4-methylcoumaryl-7-amide + H2O
-
 Oryzias latipes Boc-Glu(OBzl)-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O
-
 Oryzias latipes Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine
-
 ?
Pro-Phe-Arg-4-methylcoumaryl-7-amide + H2O
-
 Oryzias latipes 7-amino-4-methylcoumarin + Pro-Phe-Arg
-
 ?
trypsinogen + H2O
-
 Oryzias latipes trypsin + ?
-
 ?
Z-Phe-Arg-4-methylcoumaryl-7-amide + H2O
-
 Oryzias latipes 7-amino-4-methylcoumarin + Z-Phe-Arg
-
 ?

Subunits

Subunits Comment Organism
multimer 10 * 44000, gel filtration Oryzias latipes

Synonyms

Synonyms Comment Organism
enteropeptidase EP Oryzias latipes
EP-1
-
 Oryzias latipes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 loss of 30% of activity after 4 days Oryzias latipes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Oryzias latipes