Literature summary for 3.4.21.9 extracted from

Kubitzki, T.; Minoer, D.; Mackfeld, U.; Oldiges, M.; Noll, T.; Luetz, S.
Application of immobilized bovine enterokinase in repetitive fusion protein cleavage for the production of mucin 1 (2009), Biotechnol. J., 4, 1610-1618.

Search for more literature for 3.4.21.9

Application

Application	Comment	Organism
synthesis	a huge number of therapeutic proteins such as antibodies, coagulation factors, growth hormones or vaccines are produced as fusion proteins. To obtain the therapeutic protein in its monomeric, active form, the fusion partner has to be removed either by chemical or enzymatic cleavage. Enterokinase is a very attractive tool for the in vitro cleavage of fusion proteins	Bos taurus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ÄKTA-purifier system and a Sepharose QFF column (HiPrep 16/10 QFF)	Bos taurus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O	-	Bos taurus	Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine	-	?
mucin 1-IgG2a Fc + H2O	cleavage of the mucin fusion protein	Bos taurus	mucin 1 + ?	-	?

Synonyms

Synonyms	Comment	Organism
enterokinase	-	Bos taurus

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Release 2023.1 (January 2023)